Comparison of PLA Microparticles and Alum as Adjuvants for H5N1 Influenza Split Vaccine: Adjuvanticity Evaluation and Preliminary Action Mode Analysis

Zhang, Weifeng; Wang, Lianyan; Liu, Yuan; Chen, Xiaoming; Li, Jiahui; Yang, Tingyuan; An, Wenqi; Ma, Xiaowei; Pan, R Martin Du; Ma, Guanghui

doi:10.1007/s11095-013-1224-z

Cited by 39 publications

(25 citation statements)

References 52 publications

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“…1a) [12]. Briefly, 650 mg PLGA dissolved in 13 mL dichloromethane was added into 65 mL PVA solution (1.5%, m/v) and magnetically stirred at 450 rpm for 90 s to form coarse emulsion.…”

Section: Preparation and Characterization Of Plga Microparticles And mentioning

confidence: 99%

“…To further evaluate the efficacy of particles-adjuvanted H5N1 influenza vaccine, the HI assay was performed to detect anti-HA antibody levels, according to previously described method [12].…”

Section: Hemagglutination Inhibition (Hi) Assaymentioning

confidence: 99%

“…To perform this study, blank and IMQ-loaded PLGA microparticles were prepared by O/W emulsion-solvent evaporation method combined with the premix SPG membrane emulsification technique, as shown in Fig. 1a [12]. The diameters of blank PLGA microparticles (PMPs) and IMQ-loaded PLGA microparticles (IPMPs) were 1038±12.41 and 979.0±19.73 nm, respectively (Table I, Fig.…”

Section: Characteristics Of Plga Microparticles and Vaccine Formulationsmentioning

confidence: 99%

“…IgG is consisted of structurally and functionally distinct subclasses, and IgG1, IgG2a, and IgG2b are main IgG subclasses induced by protein antigens [12,22]. IgG2a and IgG2b are the most effective in activating immune cells (such as macrophages and monocytes), and ratios of IgG2a/IgG1 and IgG2b/IgG1 indicate the Th1/Th2 polarization [12,22,23]. Therefore, we analyzed the ratios of IgG2a/IgG1 and IgG2b/IgG1 for IgG antibody elicited by different vaccine formulations.…”

Section: Systemic Antibody Responses In Vaccinated Micementioning

confidence: 99%

“…pattern recognition receptors) on innate immune cells [1,[8][9][10]. Particles-based adjuvants act by combined mechanisms, such as having comparable size to pathogens which facilitates recognition and phagocytosis by APCs, activating NALP3 inflammasome of APCs, and regulating the pathway of antigen presentation [11,12]. Particles antigen delivery systems could also induce cellular immune responses.…”

Section: Introductionmentioning

confidence: 99%

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Immunopotentiator-Loaded Polymeric Microparticles as Robust Adjuvant to Improve Vaccine Efficacy

et al. 2015

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“…1a) [12]. Briefly, 650 mg PLGA dissolved in 13 mL dichloromethane was added into 65 mL PVA solution (1.5%, m/v) and magnetically stirred at 450 rpm for 90 s to form coarse emulsion.…”

Section: Preparation and Characterization Of Plga Microparticles And mentioning

confidence: 99%

Section: Hemagglutination Inhibition (Hi) Assaymentioning

confidence: 99%

Section: Characteristics Of Plga Microparticles and Vaccine Formulationsmentioning

confidence: 99%

Section: Systemic Antibody Responses In Vaccinated Micementioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Immunopotentiator-Loaded Polymeric Microparticles as Robust Adjuvant to Improve Vaccine Efficacy

et al. 2015

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Pathogen‐Mimicking Polymeric Nanoparticles based on Dopamine Polymerization as Vaccines Adjuvants Induce Robust Humoral and Cellular Immune Responses

Liu

Jia

Yang

et al. 2016

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Aiming to enhance the immunogenicity of subunit vaccines, a novel antigen delivery and adjuvant system based on dopamine polymerization on the surface of poly(D,L-lactic-glycolic-acid) nanoparticles (NPs) with multiple mechanisms of immunity enhancement is developed. The mussel-inspired biomimetic polydopamine (pD) not only serves as a coating to NPs but also functionalizes NP surfaces. The method is facile and mild including simple incubation of the preformed NPs in the weak alkaline dopamine solution, and incorporation of hepatitis B surface antigen and TLR9 agonist unmethylated cytosine-guanine (CpG) motif with the pD surface. The as-constructed NPs possess pathogen-mimicking manners owing to their size, shape, and surface molecular immune-activating properties given by CpG. The biocompatibility and biosafety of these pathogen-mimicking NPs are confirmed using bone marrow-derived dendritic cells. Pathogen-mimicking NPs hold great potential as vaccine delivery and adjuvant system due to their ability to: 1) enhance cytokine secretion and immune cell recruitment at the injection site; 2) significantly activate and maturate dendritic cells; 3) induce stronger humoral and cellular immune responses in vivo. Furthermore, this simple and versatile dopamine polymerization method can be applicable to endow NPs with characteristics to mimic pathogen structure and function, and manipulate NPs for the generation of efficacious vaccine adjuvants.

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Biodegradable Polymeric Nanocarrier-Based Immunotherapy in Hepatitis Vaccination

Hong

Ahn

Lee

et al. 2018

Advances in Experimental Medicine and Biology

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Comparison of PLA Microparticles and Alum as Adjuvants for H5N1 Influenza Split Vaccine: Adjuvanticity Evaluation and Preliminary Action Mode Analysis

Abstract: Considering the adjuvanticity and side effects (local inflammation) of both adjuvants, we conclude that PLA microparticles are promising alternative adjuvant for H5N1 influenza split vaccine.

Cited by 39 publications

References 52 publications

Immunopotentiator-Loaded Polymeric Microparticles as Robust Adjuvant to Improve Vaccine Efficacy

Immunopotentiator-Loaded Polymeric Microparticles as Robust Adjuvant to Improve Vaccine Efficacy

Pathogen‐Mimicking Polymeric Nanoparticles based on Dopamine Polymerization as Vaccines Adjuvants Induce Robust Humoral and Cellular Immune Responses

Biodegradable Polymeric Nanocarrier-Based Immunotherapy in Hepatitis Vaccination

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