COMPARISON OF POLYPEPTIDE PROFILE OF Trypanosoma evansi ISOLATES FROM INDONESIA AND THEIR RELATION TO BIOTYPE AND SENSITIVITY TO TRYPANOCIDAL

Abstract

This study aimed to determine whether the variant or biotype of Trypanosoma evansi can be seen from their polypeptide profiles using 12% sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS PAGE) stained with Brilliant Blue Commasie. The results generally showed that the molecular weight (MW) of polypeptides from nine isolates from East Java, Central Java, Banten, South Kalimantan, Central Kalimantan, and Lampung provinces were in the range of 85.46 to 15.76 kD and each isolate has different polypeptide profile. Isolates A13 and A14 were isolated from the same place but have different polypeptide profiles. Likewise, isolates S13 and S18 also have different polypeptide profiles despite being isolated from the same place at the same time. On the other hand, isolate 372, 87, and 06 have different protein profiles but was classified in the same biotype namely biotype I. Generally, the difference in protein profile actually more related to the biological diversity of the metabolism of each Trypanosoma evansi isolate from Indonesia. ____________________________________________________________________________________________________________________ ABSTRAKPenelitian ini bertujuan mengetahui varian atau biotipe Trypanosoma evansi dari profil protein menggunakan metode sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS PAGE) 12% dengan pewarnaan Commasie Brilliant Blue. Hasil penelitian secara umum menunjukkan bahwa berat molekul (BM) protein sembilan isolat dari provinsi Jawa Timur, Jawa Tengah, Banten, Kalimantan Selatan, Kalimantan Tengah, dan Lampung berada di kisaran 85,76 kD dan masing-masing isolat mempunyai profil protein yang berbeda. Isolat A13 dan A14 yang diisolasi dari tempat yang sama mempunyai profil protein yang berbeda. Demikian juga dengan isolat S13 dan S18 yang berbeda profil proteinnya meskipun diisolasi dari tempat dan waktu yang sama. Di sisi lain, isolat 372, isolat 87 dan isolat 06 mempunyai profil protein yang berbeda tetapi termasuk dalam biotipe yang sama, yaitu biotipe 1. Secara umum, perbedaan profil protein lebih berkaitan dengan keragaman biologis dari metabolisme setiap isolat Trypanosoma evansi dari Indonesia.