Comparison of PrestoBlue and MTT assays of cellular viability in the assessment of anti-proliferative effects of plant extracts on human endothelial cells

Boncler, Magdalena; Różalski, Marcin; Krajewska, Urszula; Podsędek, Anna; Watała, Cezary

doi:10.1016/j.vascn.2013.09.003

Cited by 175 publications

(126 citation statements)

References 27 publications

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“…However, MTT assay is one of the most commonly-used preliminary in-vitro cytotoxicity assays. Also, a prior study showed that the MTT test has lower inter-assay variability compared to a PB assay [52]. In a future comprehensive study, it is recommended to perform cytotoxicity tests using fluorescence-based assays like the PB method.…”

Section: Addressing Potential Limitationsmentioning

confidence: 99%

Osteogenesis and cytotoxicity of a new Carbon Fiber/Flax/Epoxy composite material for bone fracture plate applications

Bagheri

Giles

Sawi

et al. 2015

Materials Science and Engineering: C

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Section: Addressing Potential Limitationsmentioning

confidence: 99%

Osteogenesis and cytotoxicity of a new Carbon Fiber/Flax/Epoxy composite material for bone fracture plate applications

Bagheri

Giles

Sawi

et al. 2015

Materials Science and Engineering: C

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“…The cell viability was measured by the MTT assay (Boncler et al, 2014). HCE cells were seeded on 96-well plate at DOI: 10.3109/10717544.2014.991952 the initial density of 1 Â 10 4 cells per well.…”

Section: Analysis Of In Vitro Cytotoxicitymentioning

confidence: 99%

Preparation and ocular pharmacokinetics of hyaluronan acid-modified mucoadhesive liposomes

Lin

Wang

et al. 2014

Drug Delivery

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The aim of this research was to formulate a liposomal preparation of DOX to be applied topically, and to investigate the in vitro and in vivo performance of the prepared liposomes. DOX liposomes were prepared by the solvent evaporation method, and then modified with bioadhesive material HA. Through MTT assay, we found that the safe concentration of liposomes delivered would hit 1 mg/mL. Cellular uptake studies showed that DOX liposomes coated with HA are much more targetable to cell nucleus. Their ocular pharmacokinetics in rabbits were investigated through the comparison with those obtained after dosing with nonmodified liposomes and DOX solution. The in vitro transcorneal permeability of DOX in both kinds of liposomes was found to be slower than that of the solution because of sustained release. After in vivo instillation in rabbits, HA-modified liposomes had the longest retention time, following with naked liposomes. Significantly, the area under the curve of the aqueous humor concentration-time profiles of DOX liposomes was found to be 1.7-fold higher than that of DOX solution. The confocal experiment confirmed that HA-modified liposomes were able to maintain a higher DOX concentration and residence time than that of non-modified liposomes and free DOX. These results suggest that our liposomal preparation was of great help to improve the bioavailability of DOX.

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“…This assay is commercially available and comes ready to use. 24 Metabolically active cells are capable of reducing the PrestoBlue reagent, with the colorimetrically measured change used to quantify cell viability. Our assay was performed according to the manufacturer's protocol.…”

Section: Methodsmentioning

confidence: 99%

Human Alveolar Epithelial Cell Responses to Core–Shell Superparamagnetic Iron Oxide Nanoparticles (SPIONs)

Mbeh¹,

Mireles²,

Stanicki

et al. 2015

Langmuir

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Superparamagnetic iron oxide nanoparticles (SPIONs) have been prepared and coated with positively (-NH3(+)) and negatively (-COO(-)) charged shells. These NPs, as well as their "bare" precursor, which actually contain surface hydroxyl groups, have been characterized in vitro, and their influence on a human epithelial cell line has been assessed in terms of cell metabolic activity, cellular membrane lysis, mitochondrial activity, and reactive oxygen species production. Their physicochemical characterizations and protein-nanoparticle interactions have been determined using dynamic light scattering, high-resolution transmission electron microscopy, matrix-assisted laser desorption/ionization time-of-flight (MALDI-TOF) spectrometry, and Coomassie Blue fast staining. Cell-SPION interactions have been determined by PrestoBlue resazurin-based, Trypan Blue dye exclusion-based, and MTS cell proliferation assays as well as by reactive oxygen species determination. The results show that different surface characteristics cause different protein corona and cell responses. Some proteins (e.g., albumin) are adsorbed only on positively charged coatings and others (e.g., fibrinogen) only on negatively charged coating. No cell deaths occur, but cell proliferation is influenced by surface chemistry. Proliferation reduction is dose dependent and highest for bare SPIONs. Negatively charged SPIONs were the most biocompatible.

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Comparison of PrestoBlue and MTT assays of cellular viability in the assessment of anti-proliferative effects of plant extracts on human endothelial cells

Cited by 175 publications

References 27 publications

Osteogenesis and cytotoxicity of a new Carbon Fiber/Flax/Epoxy composite material for bone fracture plate applications

Osteogenesis and cytotoxicity of a new Carbon Fiber/Flax/Epoxy composite material for bone fracture plate applications

Preparation and ocular pharmacokinetics of hyaluronan acid-modified mucoadhesive liposomes

Human Alveolar Epithelial Cell Responses to Core–Shell Superparamagnetic Iron Oxide Nanoparticles (SPIONs)

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