Comparison of preventive and therapeutic effects of continuous exercise on acute lung injury induced with methotrexate

Rajizadeh, Mohammad‐Amin; Hosseini, Mahdiyeh Haj; Bahrami, Mina; Hosseini, Najmeh Sadat; Rostamabadi, Fahimeh; Bagheri, Fatemeh; Khoramipour, Kayvan; Najafipour, Hamid; Bejeshk, Mohammad‐Abbas

doi:10.1113/ep091162

Cited by 12 publications

(6 citation statements)

References 47 publications

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“…The significant increase in MPO in the brain regions can affect the development of neurodegeneration [ 52 ]. Reduced transcription of GATA3 and absence of MPO in the brains infected with NS80 viruses may be beneficial [ 53 ]. It was recently shown that cognitive, memory, and proinflammatory activities were improved in the Alzheimer’s disease mouse model (5XFAD) after producing bone marrow deficient MPO [ 54 ].…”

Section: Discussionmentioning

confidence: 99%

Changes in the Expression of Proteins Associated with Neurodegeneration in the Brains of Mice after Infection with Influenza A Virus with Wild Type and Truncated NS1

Donátová,

Mladá,

Lopušná

et al. 2024

IJMS

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Influenza type A virus (IAV) infection is a major cause of morbidity and mortality during influenza epidemics. Recently, a specific link between IAV infection and neurodegenerative disease progression has been established. The non-structural NS1 protein of IAV regulates viral replication during infection and antagonizes host antiviral responses, contributing to influenza virulence. In the present study, we have prepared a mouse lung-to-lung adapted to the NS1-truncated virus (NS80ad). Transcriptome analysis of the gene expression in the lungs revealed that infection with wild-type A/WSN/33 (WSN), NS80, and NS80ad viruses resulted in different regulation of genes involved in signaling pathways associated with the cell proliferation, inflammatory response, and development of neurodegenerative diseases. NS1 protein did not influence the genes involved in the RIG-I-like receptor signaling pathway in the brains. Lethal infection with IAVs dysregulated expression of proteins associated with the development of neurodegenerative diseases (CX3CL1/Fractalkine, Coagulation factor III, and CD105/Endoglin, CD54/ICAM-1, insulin-like growth factor-binding protein (IGFBP)-2, IGFBP-5, IGFBP-6, chitinase 3-like 1 (CHI3L1), Myeloperoxidase (MPO), Osteopontin (OPN), cystatin C, and LDL R). Transcription of GATA3 mRNA was decreased, and expression of MPO was inhibited in the brain infected with NS80 and NS80ad viruses. In addition, the truncation of NS1 protein led to reduced expression of IGFBP-2, CHI3L1, MPO, and LDL-R proteins in the brains. Our results indicate that the influenza virus influences the expression of proteins involved in brain function, and this might occur mostly through the NS1 protein. These findings suggest that the abovementioned proteins represent a promising target for the development of potentially effective immunotherapy against neurodegeneration.

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Section: Discussionmentioning

confidence: 99%

Changes in the Expression of Proteins Associated with Neurodegeneration in the Brains of Mice after Infection with Influenza A Virus with Wild Type and Truncated NS1

Donátová,

Mladá,

Lopušná

et al. 2024

IJMS

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“…In our prior research, we demonstrated the protective effects of continuous aerobic exercise against methotrexate-induced lung injury [ 25 ]. In this study, our focus was to evaluate the impact of High-Intensity Interval Training (HIIT), characterized by brief periods of intense anaerobic exercise alternated with short recovery periods, on methotrexate-induced lung injury.…”

Section: Discussionmentioning

confidence: 99%

“…This assessment was performed without knowledge of the animal groups, ensuring objectivity. The grading of lung injury was based on the evaluation of histological features [ 25 , 26 ].…”

Section: Methodsmentioning

confidence: 99%

High-intensity intermittent training ameliorates methotrexate-induced acute lung injury

Rajizadeh,

Hosseini,

Bahrami

et al. 2024

BMC Pulm Med

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Inflammation and oxidative stress are recognized as two primary causes of lung damage induced by methotrexate, a drug used in the treatment of cancer and immunological diseases. This drug triggers the generation of oxidants, leading to lung injury. Given the antioxidant and anti-inflammatory effects of high-intensity intermittent training (HIIT), our aim was to evaluate the therapeutic potential of HIIT in mitigating methotrexate-induced lung damage in rats. Seventy male Wistar rats were randomly divided into five groups: CTL (Control), HIIT (High-intensity intermittent training), ALI (Acute Lung Injury), HIIT+ALI (pretreated with HIIT), and ALI + HIIT (treated with HIIT).HIIT sessions were conducted for 8 weeks. At the end of the study, assessments were made on malondialdehyde, total antioxidant capacity (TAC), superoxide dismutase (SOD), glutathione peroxidase (Gpx), myeloperoxidase (MPO), interleukin 10 (IL-10), tumor necrosis factor-alpha (TNF-α), gene expression of T-bet, GATA3, FOXP3, lung wet/dry weight ratio, pulmonary capillary permeability, apoptosis (Caspase-3), and histopathological indices.Methotrexate administration resulted in increased levels of TNF-α, MPO, GATA3, caspase-3, and pulmonary edema indices, while reducing the levels of TAC, SOD, Gpx, IL-10, T-bet, and FOXP3. Pretreatment and treatment with HIIT reduced the levels of oxidant and inflammatory factors, pulmonary edema, and other histopathological indicators. Concurrently, HIIT increased the levels of antioxidant and anti-inflammatory factors.

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“…For the immune detection step, a Chemi Doc XRS + imaging system (Bio-Rad Company, USA) was utilized. Subsequently, the resulting images were subjected to meticulous analysis employing ImageJ software 49 , 50 . Notably, β-Actin (SANTA CRUZ, sc-47778) served as the designated housekeeping protein 51 , 52 .…”

Section: Methodsmentioning

confidence: 99%

ATP releasing channels and the ameliorative effects of high intensity interval training on diabetic heart: a multifaceted analysis

Joukar,

Rajizadeh,

Bejeshk

et al. 2024

Sci Rep

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Type 2 diabetes (T2D) can cause severe cardiac complications at functional, histologic and molecular levels. These pathological complications could be mediated by ATP-releasing channels such as Panx1 and ATP receptors, in particular P2X7. The aim of our study was to investigate the effect of high-intensity interval training (HIIT) on T2D-induced cardiac complications at the functional, histopathological and molecular levels, with a particular focus on ATP-releasing channels. 48 male Wistar rats at the age of 8 weeks were randomly allocated into four groups: control (Con), Diabetes (T2D), Training (TR), and Diabetes + Training (T2D + TR). T2D was induced by a high-fat diet plus a low dose (35 mg/kg) of STZ administration. Rats in the TR and T2D + TR groups underwent an 8-weeks training program involving intervals ranging from 80 to 100% of their maximum running speed (Vmax), with 4–10 intervals per session. Protein expression of Interleukin 1β (IL1β), Interleukin 10 (IL-10), Pannexin 1 (Panx1), P2X7R (purinergic P2X receptor 7), NLRP1 (NLR Family Pyrin Domain Containing 1), BAX, and Bcl2 were measured in the heart tissue. Additionally, we assessed heart function, histopathological changes, as well as insulin resistance using the homeostasis model assessment of insulin resistance (HOMA-IR). In contrast to the T2D group, HIIT led to increased protein expression of Bcl2 and IL-10 in the heart. It also resulted in improvements in systolic and diastolic blood pressures, heart rate, ± dp/dt (maximum and minimum changes in left ventricular pressure), while reducing protein expression of IL-1β, Panx1, P2X7R, NLRP1, and BAX levels in the heart. Furthermore, left ventricular diastolic pressure (LVDP) was reduced (P ≤ 0.05). Moreover, heart lesion scores increased with T2D but decreased with HIIT, along with a reduction in fibrosis percentage (P ≤ 0.05). The results of this study suggest that the cardioprotective effects of HIIT on the diabetic heart may be mediated by the modulation of ATP-releasing channels. This modulation may lead to a reduction in inflammation and apoptosis, improve cardiac function, and attenuate cardiac injury and fibrosis.

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Comparison of preventive and therapeutic effects of continuous exercise on acute lung injury induced with methotrexate

Cited by 12 publications

References 47 publications

Changes in the Expression of Proteins Associated with Neurodegeneration in the Brains of Mice after Infection with Influenza A Virus with Wild Type and Truncated NS1

Changes in the Expression of Proteins Associated with Neurodegeneration in the Brains of Mice after Infection with Influenza A Virus with Wild Type and Truncated NS1

High-intensity intermittent training ameliorates methotrexate-induced acute lung injury

ATP releasing channels and the ameliorative effects of high intensity interval training on diabetic heart: a multifaceted analysis

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