Comparison of profiles of key periodontal pathogens in periodontium and endodontium

Abstract

Despite the established anatomical relationship between the periodontal and pulpal tissues, bacterial migration between endodontium and periodontium is still under discussion. The objective of this study was an investigation of profiles of periodontal pathogens in pulpal and periodontal diseases affecting the same tooth by means of 16S rRNA gene directed polymerase chain reaction (PCR). 31 intact teeth with both pulp and marginal infections were investigated. The diagnosis was based on clinical and radiological examination. Samples were taken from the gingival sulcus or periodontal pocket, respectively, with sterile paper points before trepanation of the teeth. After trepanation sterile paper points and Hedstroem files were used for taking samples from the root canal. Specific PCR methods were used to detect the presence of the following pathogens: Actinobacillus actinomycetemcomitans, Bacteroides forsythus, Eikenella corrodens, Fusobacterium nucleatum, Porphyromonas gingivalis, Prevotella intermedia and Treponema denticola. In addition, quantitative competitive PCR was used to determine the total bacterial count of the samples. The investigated pathogens were proven to be present in the endondontium in all disease categories. Particularly in endodontic samples of "chronic apical periodontitis" and "chronic adult periodontitis" profiles of the periodontal pathogens were found. The results confirmed that periodontal pathogens often accompany endodontic infections and supported the idea that the periodontic-endodontic interrelationships should be considered as critical pathways which might contribute to refractory courses of endodontic or periodontal diseases.

Neuropeptide Release from Dental Pulp Cells by RgpB via Proteinase-Activated Receptor-2 Signaling

“…Therefore, released CGRP and SP contents in 10,000 HPC/0.1 ml are 20.8 and 18.2 pg, respectively. Under an assumption that a HPC is a ball-like structure with a diameter of 10 m, a HPC volume [3/4 ϫ 3.14 ϫ (0.0005 cm) 3 ] is estimated to be 0.3 ϫ 10 Ϫ9 ml. Supposing that a neuropeptide evenly distributes in a HPC, CGRP and SP concentrations in a cell are 7 g/ml (1.8 M) and 6 g/ml (4.6 M), respectively.…”

“…Cell surface-associated and secretory trypsin-like cysteine proteinases (gingipains) produced by P. gingivalis are important virulence factors and are implicated in the development of periodontitis (1,2). Periodontitis often leads to acute pulpitis and pulpal necrosis by dissemination of the pathogens and/or their virulence components (3)(4)(5)(6)(7)(8)(9)(10). Arginine-specific cysteine protease (RgpB) 2 is a gingipain (11) and has various proinflammatory activities including vascular permeability enhancement and edema induction (1,2), which can occur in acute pulpitis (12,13).…”

In vitro evaluation of the antimicrobial activity of nanosilver-mineral trioxide aggregate against frequent anaerobic oral pathogens by a membrane-enclosed immersion test

“…intermedia could be accompanying endodontic infections due to the periodontic-endodontic interrelationships. [23] Periodontal disease is a frequently prevalent oral health problem with wide variations in severity and prevalence across different geographic areas. In adult periodontitis, Po.…”

Endo-Perio Symbiosis

“…Microbiology of endo-perio lesions includes bacterial species like Actinobacillus Actinomycetem comitans, Bacteroides forsythus, Ekinella corrodens, Fusobacterium nucleatum, Porphyrominas gingivalis, Prevotella intermedia (Rupf et al, 2000). Also fungal species like Candida albicans (Hannula et al, 1997) are predominant in endodontic and periodontal lesions.…”