Comparison of proliferation and osteogenic differentiation potential of bovine adipose tissue and bone marrow derived stem cells

Akkaya, Özlem; Dikmen, Tayfun; Nawaz, Shah; Kibria, Asm Golam; Altunbaş, Korhan; Yağcı, A.; Erdoğan, Metin; Yaprakci, Mustafa Volkan

doi:10.1080/10520295.2023.2177347

Cited by 2 publications

(1 citation statement)

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“…In particular, expression of CD105 drastically decreases in MSCs derived from ovine and goat species, while DC166 expression is decreased in MSCs derived from mouse [29]. Additionally, bovine-derived MSCs surface marker expression can vary depending on the tissue source [39][40][41]. Mesenchymal stem cells derived from bovine fetal adipose tissue had a significantly higher expression of CD90 than CD73 and CD105 [39], suggesting that the surface marker expression pattern reported in this study is native to BvAdMSCs.…”

Section: Discussionmentioning

confidence: 59%

Phenotypic and Functional Characterization of Bovine Adipose-Derived Mesenchymal Stromal Cells

Maldonado,

Pokharel,

Powell

et al. 2024

Animals

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Background: Mesenchymal stem cells (MSCs) are increasingly trialed in cellular therapy applications in humans. They can also be applied to treat a range of diseases in animals, particularly in cattle to combat inflammatory conditions and aging-associated degenerative disorders. We sought to demonstrate the feasibility of obtaining MSCs from adipose tissue and characterizing them using established assays. Methods: Bovine adipose MSCs (BvAdMSCs) were isolated using in-house optimized tissue digestion protocols and characterized by performing a colony formation assay, cell growth assessments, cell surface marker analysis by immunocytochemistry and flow cytometry, osteogenic and adipogenic differentiation, and secretion of indoleamine 2,3-dioxygenease (IDO). Results: Our results demonstrate the feasibility of successful MSC isolation and culture expansion from bovine adipose tissues with characteristic features of colony formation, in vitro multilineage differentiation into osteogenic and adipogenic lineages, and cell surface marker expression of CD105, CD73, CD90, CD44, and CD166 with negative expression of CD45. BvAdMSCs secreted significant amounts of IDO with or without interferon–gamma stimulation, indicating ability for immunomodulation. Conclusions: We report a viable approach to obtaining autologous adipose-derived MSCs that can be applied as potential adjuvant cell therapy for tissue repair and regeneration in cattle. Our methodology can be utilized by veterinary cell therapy labs for preparing MSCs for disease management in cattle.

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Section: Discussionmentioning

confidence: 59%

Phenotypic and Functional Characterization of Bovine Adipose-Derived Mesenchymal Stromal Cells

Maldonado,

Pokharel,

Powell

et al. 2024

Animals

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Establishment of immortalized rabbit bone marrow mesenchymal stem cells and a preliminary study of their osteogenic differentiation capability

Zhang,

Xu,

Huang

et al. 2024

Anim Models and Exp Med

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BackgroundA stable and standardized source of mesenchymal stem cells is a prerequisite for bone repair tissue engineering research and application. We aimed to establish a stable cell line of bone marrow mesenchymal stem cells from New Zealand rabbits and explore their osteogenic differentiation capacity.MethodsPrimary rabbit bone marrow mesenchymal stem cells (RBMSCs) were isolated and immortalized via retroviral expression of SV40 Large T antigen (LTA). To assess the osteogenic differentiation capacity of the cells in vitro, we studied the alkaline phosphatase (ALP) expression level and calcium deposition in bone morphogenetic protein 9 (BMP9)‐induced immortalized cells using ALP staining and quantification, as well as alizarin red staining. Ectopic bone formation by the cells was assessed using micro‐computed tomography (μCT) and histological examination.ResultsThe immortalized cell line we established using SV40 LTA, which we termed iRBMSCs, was non‐tumorigenic and maintained long‐term proliferative activity. We further discovered that BMP9 (MOI = 30) effectively induced the osteogenic differentiation capacity of iRBMSCs in vitro, and there was a synergy with GelMA hydrogel in inducing osteogenic differentiation of the iRBMSCs in vivo.ConclusionWe confirmed that iRBMSCs are promising as a stable cell line source for bone defect repair engineering.

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Comparison of proliferation and osteogenic differentiation potential of bovine adipose tissue and bone marrow derived stem cells

Cited by 2 publications

References 55 publications

Phenotypic and Functional Characterization of Bovine Adipose-Derived Mesenchymal Stromal Cells

Phenotypic and Functional Characterization of Bovine Adipose-Derived Mesenchymal Stromal Cells

Establishment of immortalized rabbit bone marrow mesenchymal stem cells and a preliminary study of their osteogenic differentiation capability

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