Comparison of proliferative activity of Wharton jelly mesenchymal stem cells in cultures under various gas conditions

Shuvalova, N. S.; Кордюм, В. А.

doi:10.7124/bc.0008e5

Cited by 6 publications

(20 citation statements)

References 35 publications

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“…The enlarged cells which have flattened shape (though without noticeable presence of granules and vacuoles in cytoplasm), reflecting typical senescent morphology, were observed in significant amounts at the passage 3. This correlated with the drop of proliferation rate demonstrated in our previous works [20]. Notably, plating density used in the present study can be considered as relatively low [28,29].…”

Section: Passagesupporting

confidence: 89%

“…MSC isolation. MSC were isolated from human Wharton jelly (WJ) using the explants method, as described in our previous work [20]. Umbilical cords (UC) were collected from three healthy donors (39-40 weeks of gestation, normal delivery), after obtaining informed consent.…”

Section: Methodsmentioning

confidence: 99%

“…Starting from the passage 1, WJ-MSC were replated (as described above) and expanded for 5 subsequent passages (for 7 days at each passage) in complete growth media. All cultures were replated simultaneously and at the same cell density, as described in our previous work [20].…”

Section: Methodsmentioning

confidence: 99%

“…The containers with bags were kept incubated at 37 °C. The percentage "liquid media/gas" for normal gas exchange must be 1:100 [21], the volu me of gas mixture must be not less than 1 l [20].…”

Section: Methodsmentioning

confidence: 99%

“…In general, the overall number of cells with altered morphology, predominantly the ones with a broad flattened shape, at the passage 3 was lower in gas mixtures, than in CO 2 -incubator. Furthermore, in gas mixtures the cultures underwent more population doublings (PD), comparing to the control group under standard conditions [20,30]. The work by Ren et al [31] describes the prominent senescence-associated morphologic changes in the cultures under mild hypoxic conditions.…”

Section: Passagementioning

confidence: 99%

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Morphological characteristics of mesenchymal stem cells from Wharton jelly, cultivated under physiological oxygen tensions, in various gas mixtures

Shuvalova

Кордюм

2016

Biopolym. Cell

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Cultivation of mesenchymal stem cells (MSC) under physiological oxygen tensions has beneficial effect on their properties. The aim of the present work was to examine the effects of mild hypoxic conditions on the morphologic behavior of MSC from human Wharton jelly (WJ-MSC). Methods. WJ-MSC were obtained by an explant method and cultured in gas mixtures containing 3 % O 2 , 4 % CO 2 and 93 % nitrogen or argon. At each passage the cultures were fixed and stained with hematoxylin and eosin. The morphometric analysis of microphotographs was performed, and the nuclear-cytoplasmic ratio (NCR) and the width/length ratio were calculated. Results. The difference in NCR and the width/length ratio was detected between WJ-MSC cultures at various passages and between the cultures at the same passage, maintained in different gas mixtures. The highest level of morphological homogeneity was observed at passage 2. At passage 3, the cells with senescent morphology can be observed. The degree of heterogeneity in populations cultured at 3 % O 2 appeared to be lower than under standard conditions. WJ-MSC, cultured in the nitrogen-based gas mixture were the most homogeneous. Conclusions. The changes in WJ-MSC culture morphology were observed at early passages. The level of morphological heterogeneity varied with the cultivation time. The conditions of mild hypoxia had a positive impact on the cultured cells. K e y w o r d s: mesenchymal stem cells, Wharton jelly, morphology, hypoxia, argon К л юч е в ы е с л ов а: мезенхимальные стволовые клетки, Вартонов студень, гипоксия, морфология, аргон

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Section: Passagesupporting

confidence: 89%

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Passagementioning

confidence: 99%

See 3 more Smart Citations

Morphological characteristics of mesenchymal stem cells from Wharton jelly, cultivated under physiological oxygen tensions, in various gas mixtures

Shuvalova

Кордюм

2016

Biopolym. Cell

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MXene-Assisted Ablation of Cells with a Pulsed Near-Infrared Laser

Kyrylenko

Gogotsi²,

Baginskiy³

et al. 2022

ACS Appl. Mater. Interfaces

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Innovative therapies are urgently needed to combat cancer. Thermal ablation of tumor cells is a promising minimally invasive treatment option. Infrared light can penetrate human tissues and reach superficial malignancies. MXenes are a class of 2D materials that consist of carbides/nitrides of transition metals. The transverse surface plasmons of MXenes allow for efficient light absorption and light-to-heat conversion, making MXenes promising agents for photothermal therapy (PTT). To date, near-infrared (NIR) light lasers have been used in PTT studies explicitly in a continuous mode. We hypothesized that pulsed NIR lasers have certain advantages for the development of tailored PTT treatment targeting tumor cells. The pulsed lasers offer a wide range of controllable parameters, such as power density, duration of pulses, pulse frequency, and so on. Consequently, they can lower the total energy applied and enable the ablation of tumor cells while sparing adjacent healthy tissues. We show for the first time that a pulsed 1064 nm laser could be employed for selective ablation of cells loaded with Ti3C2T x MXene. We demonstrate both low toxicity and good biocompatibility of this MXene in vitro, as well as a favorable safety profile based on the experiments in vivo. Furthermore, we analyze the interaction of MXene with cells in several cell lines and discuss possible artifacts of commonly used cellular metabolic assays in experiments with MXenes. Overall, these studies provide a basis for the development of efficient and safe protocols for minimally invasive therapies for certain tumors.

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Assessment of the Neuroprotective and Stemness Properties of Human Wharton’s Jelly-Derived Mesenchymal Stem Cells under Variable (5% vs. 21%) Aerobic Conditions

Tomecka

Lech

Zychowicz

et al. 2021

Cells

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To optimise the culture conditions for human Wharton’s jelly-derived mesenchymal stem cells (hWJ-MSCs) intended for clinical use, we investigated ten different properties of these cells cultured under 21% (atmospheric) and 5% (physiological normoxia) oxygen concentrations. The obtained results indicate that 5% O2 has beneficial effects on the proliferation rate, clonogenicity, and slowdown of senescence of hWJ-MSCs; however, the oxygen level did not have an influence on the cell morphology, immunophenotype, or neuroprotective effect of the hWJ-MSCs. Nonetheless, the potential to differentiate into adipocytes, osteocytes, and chondrocytes was comparable under both oxygen conditions. However, spontaneous differentiation of hWJ-MSCs into neuronal lineages was observed and enhanced under atmospheric oxygen conditions. The cells relied more on mitochondrial respiration than glycolysis, regardless of the oxygen conditions. Based on these results, we can conclude that hWJ-MSCs could be effectively cultured and prepared under both oxygen conditions for cell-based therapy. However, the 5% oxygen level seemed to create a more balanced and appropriate environment for hWJ-MSCs.

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Comparison of proliferative activity of Wharton jelly mesenchymal stem cells in cultures under various gas conditions

Cited by 6 publications

References 35 publications

Morphological characteristics of mesenchymal stem cells from Wharton jelly, cultivated under physiological oxygen tensions, in various gas mixtures

Morphological characteristics of mesenchymal stem cells from Wharton jelly, cultivated under physiological oxygen tensions, in various gas mixtures

MXene-Assisted Ablation of Cells with a Pulsed Near-Infrared Laser

Assessment of the Neuroprotective and Stemness Properties of Human Wharton’s Jelly-Derived Mesenchymal Stem Cells under Variable (5% vs. 21%) Aerobic Conditions

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