Comparison of Protein Profiles of Gingival Crevicular Fluids Collected from Incisors, Canines, and Molars

Abstract

Many studies have shown that gingival crevicular uid GCF re ects the in ammatory state of local periodontal tissues. GCF has been collected from several types of teeth in previous studies. However, there is no report that characterizes GCF by the type of tooth. In the present study, the protein pro les of GCF from different sites were comprehensively compared with each other. GCF was sampled from six healthy adult men 21-31 years old with healthy periodontal tissues. Three separate GCF samples were collected at the maxillary central incisor, canine, and rst molar of each individual. The protein pro les of GCF were analyzed using sodium dodecyl sulfate polyacrylamide gel electrophoresis and liquid chromatogram-tandem mass spectrometry LC-MS/MS. The band patterns on the sodium dodecyl sulfate polyacrylamide gel electrophoresis from the set of three GCF samples from each individual were similar, regardless of the type of tooth. The proteins contained in each band were identi ed by LC-MS/MS analysis, and they were found to be the same among the three GCF samples. A comprehensive and quantitative analysis of proteins in the GCF samples was performed by LC-MS/MS using isobaric tag labeling. In total, 86 proteins were identi ed in GCF. A small number of proteins were increased or decreased in GCF from the rst molars compared with the other types of teeth in one or two individuals. However, overall, no proteins were found to exhibit a reproducibly different composition in any of the individuals. These analyses show that the protein pro les of GCF in healthy periodontal tissues are similar, regardless of the type of tooth.

Proteomic Analysis of Gingival Crevicular Fluid During Tooth Eruption

“…Apolipoprotein A-1 (ApoA-1) is the major protein component of high-density lipoprotein (HDL) (34). It was detected in both groups demonstrating no significant difference, similar to the studies identify ApoA-1 in their analysis (Table 4) (8,23,25,29,35). Only Moriya et al (28) reported that the ApoA-1 level was upregulated in the GCF of the permanent teeth compared to the primary teeth.…”