Comparison of radial immunodiffusion and alkaline cellulose acetate electrophoresis for quantitating elevated levels of fetal hemoglobin (HbF): Application to evaluating patients with sickle cell disease treated with hydroxyurea

Schultz, Jo El J.

doi:10.1002/(sici)1098-2825(1999)13:2<82::aid-jcla7>3.0.co;2-c

Cited by 6 publications

(4 citation statements)

References 25 publications

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“…It is worth noting that laboratory methods for measurement of HbF have generally been developed to measure fairly low levels. It is important to ensure that the method used is reliable at higher HbF concentrations (Epstein et al , 1996; Schultz, 1999).…”

Section: Dose and Monitoring Of Hydroxyureamentioning

confidence: 99%

The role of hydroxyurea in sickle cell disease

2003

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Section: Dose and Monitoring Of Hydroxyureamentioning

confidence: 99%

The role of hydroxyurea in sickle cell disease

2003

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“…Sickle cell anemia was proved by alkaline electrophoresis of hemoglobin. 17 All participants were adults over the age of 18, divided into 3 groups, and all signed a consent form in accordance with an internal review board–approved protocol at King Abdel-Aziz Hospital, Jeddah, Saudi Arabia. Participants enrolled in the study include painful crisis group comprising 36 patients, steady state group comprising 30 patients, and the control group comprising 35 healthy, age- and sex-matched donor patients (Table 1 ).…”

Section: Methodsmentioning

confidence: 99%

Section: Clinical Profile Of Enrolled Patients With Scdmentioning

confidence: 99%

Biomarkers of Inflammation, Growth Factor, and Coagulation Activation in Patients With Sickle Cell Disease

Qari

Dier

Mousa

2011

Clin Appl Thromb Hemost

108

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Acute painful crisis is a common sequela that can cause significant morbidity and negatively impact the quality of life of patients with sickle cell disease (SCD). Plasma levels of several chemokines and cytokines including tumor necrosis factor-a (TNF-a), interleukin 1b (IL-1b), IL-6, IL-8, monocyte chemoattractant protein 1 (MCP-1), macrophage inflammatory protein 1a (MIP1a), and interferon g (IFN-g) in patients with SCD showed a distinct and statistically significant rise either during painful crisis or at steady state. Plasma levels of various growth factors, including human vascular endothelial growth factor (VEGF), human basic fibroblast growth factor (FGF), and human hepatocyte growth factor (HGF), showed a sustained 2-to 3-fold increase either during painful crisis or at steady state in patients with SCD. Furthermore, plasma levels of the biomarker D-Dimer, a marker of hypercoagulation, showed a 2-to 3-fold increase either during painful crisis or at steady state in patients with SCD as compared to that in healthy participants, suggesting an increased risk of thrombosis.

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“…Th e participating subjects were recruited from patients attending the hematology clinic at King Abdulaziz University Hospital (KAUH), Jeddah, Saudi Arabia. Th e diagnosis of SCA was confi rmed by alkaline electrophoresis of hemoglobin [20] . Ethical approval was granted from the Ethical and Technical Committee at KAUH, and written informed consents were obtained from all study participants.…”

Section: Materials and Subjectsmentioning

confidence: 99%

Correlation Between the Frequency of Painful Crises in Sickle Cell Anemia and the Biomarkers of Inflammation, Endothelial Dysfunction, Coagulation Activation and Bone Metabolism

et al. 2017

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Objective: Sickle cell anemia is a widespread inherited hemolytic anemia, characterized by chronic hemolysis, infections, recurrent occlusion of the microcirculation and painful crises. In this cross-sectional study, we investigated the correlation between biomarkers of inflammation, endothelial dysfunction, coagulation activation and bone metabolism, with the frequency of painful crisis in sickle cell anemia adult patients, to understand the potential role of these biomarkers in the management of sickle cell anemia. Methods: Sixty-three sickle cell anemia patients were enrolled in this study. They were divided into three groups based on the frequency of pain crises to mildly, moderately, and severely affected. Twenty-five healthy volunteers were enrolled as controls. Plasma levels of different biomarkers were tested, including tumor necrosis factor alpha, soluble intercellular adhesion molecule-1, D-dimer, sclerostin, and nitric oxide. Results: Tumor necrosis factor alpha, soluble intercellular adhesion molecule-1, and D-Dimer plasma levels were significantly higher in sickle cell anemia patients when compared to the controls, while nitric oxide levels were notably lower in the patients' group. Sickle cell anemia patients with severe pain showed higher tumor necrosis factor alpha, soluble intercellular adhesion molecule-1 and D-dimer levels than those mildly and moderately affected with painful crisis. Positive correlations were observed between all measured biomarkers and the frequency of the painful crisis, except for nitric oxide, which showed a negative correlation. Conclusion: Tumor necrosis factor alpha, soluble intercellular adhesion molecule-1, nitric oxide, and D-dimer can be used to assess sickle cell anemia severity and prognosis, and thus may affect determining appropriate management strategies.

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Comparison of radial immunodiffusion and alkaline cellulose acetate electrophoresis for quantitating elevated levels of fetal hemoglobin (HbF): Application to evaluating patients with sickle cell disease treated with hydroxyurea

Cited by 6 publications

References 25 publications

The role of hydroxyurea in sickle cell disease

The role of hydroxyurea in sickle cell disease

Biomarkers of Inflammation, Growth Factor, and Coagulation Activation in Patients With Sickle Cell Disease

Correlation Between the Frequency of Painful Crises in Sickle Cell Anemia and the Biomarkers of Inflammation, Endothelial Dysfunction, Coagulation Activation and Bone Metabolism

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