Comparison of radioimmunoassay and liquid chromatography tandem mass spectrometry for determination of juvenile hormone titers

Chen, Zhaorigetu; Linse, Klaus D.; Taub-Montemayor, Tina E.; Rankin, Mary Ann

doi:10.1016/j.ibmb.2007.05.019

Cited by 26 publications

(44 citation statements)

References 17 publications

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“…The similar modulation of photoperiodism by temperature was reported in other aphid species including the pea aphid [reviewed in (Lees, 1966)]. LC-MS/MS analysis showed that JHIII titer in aphids reared at 25°C was about twice that of aphids reared at 10 °C (Chen et al, 2007). Furthermore, we showed that JHIII titers in aphids reared in long-day conditions were more than two times higher than those of aphids under short-days.…”

Section: Discussionsupporting

confidence: 87%

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Juvenile hormone titre and related gene expression during the change of reproductive modes in the pea aphid

Ishikawa

Ogawa

Gotoh

et al. 2011

Insect Molecular Biology

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Most aphids show reproductive polyphenism, i.e. they alternate their reproductive modes from parthenogenesis to sexual reproduction in response to short photoperiod. Although juvenile hormone (JH) has been considered a likely candidate for regulating the transition from asexual to sexual reproduction after photoperiod sensing, there are few studies investigating the direct relationship between JH titers and the reproductive mode change. In addition, the sequencing of the pea aphid genome now allows identification of the genes involved in the JH pathway which then allows us to examine their expression levels in relation to the reproductive-mode switch. Using LC-MS in the pea aphid, JHIII titer was shown to be lower in aphids producing sexual morphs under short-days than in aphids producing parthenogenetic morphs in long-days. The expression levels of genes upstream and downstream of JH action were quantified by real-time qRT-PCR across the reproductive mode change. The expression level of JH esterase (JHE), which is responsible for JH degradation, was significantly higher in aphids reared under short-days. This suggests that the up-regulation of the JH degradation pathway may be responsible for the lower JHIII titer in aphids exposed to short-days, leading to the production of sexual morphs.

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Section: Discussionsupporting

confidence: 87%

“…The sample was stored at -20°C until analysis. Experimental details were modified from Chen et al, 2007. To quantify juvenile hormone titers in aphids, the experimental methods of LC-MS were slightly modified based on Westerlund et al, 2004 andCornette et al, 2008. Briefly, 5 …”

Section: Jh Quantificationmentioning

confidence: 99%

Juvenile hormone titre and related gene expression during the change of reproductive modes in the pea aphid

Ishikawa

Ogawa

Gotoh

et al. 2011

Insect Molecular Biology

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“…However, RIAs require specific antibodies for each JH subtype, and the results of these assays may not coincide with those of physicochemical analyses [16] due mainly to the cross-reactivity of the antiserum with other substances present in biological samples. Moreover, LC-MS/MS appears to be slightly more sensitive than RIA in determining JH titers [17] and as JH forms present in biological samples can be identified by LC-MS/MS, it may be more selective than RIA. However, as only one JH isoform (JH III) was analyzed here and in the previously mentioned study [17] the issue of sensitivity remains to be confirmed.…”

Section: Introductionmentioning

confidence: 99%

“…Moreover, LC-MS/MS appears to be slightly more sensitive than RIA in determining JH titers [17] and as JH forms present in biological samples can be identified by LC-MS/MS, it may be more selective than RIA. However, as only one JH isoform (JH III) was analyzed here and in the previously mentioned study [17] the issue of sensitivity remains to be confirmed.…”

Section: Introductionmentioning

confidence: 99%

Liquid chromatography coupled to ion trap-tandem mass spectrometry to evaluate juvenile hormone III levels in bee hemolymph from Nosema spp. infected colonies

Ares

Nozal

Bernal

et al. 2012

Journal of Chromatography B

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“…Although these have been validated for use in honey bees (Zhou et al, 2011) and are comparable in terms of sensitivity to radioimmunoassays and GC-MS (Chen et al, 2007) 1. For collecting haemolymph from feeding-stage larvae:…”

Section: Analysis Of Juvenile Hormone and Ecdysteroid Levels In Honeymentioning

confidence: 99%

Standard methods for physiology and biochemistry research inApis mellifera

Hartfelder

Bitondi

Brent

et al. 2013

Journal of Apicultural Research

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SummaryDespite their tremendous economic importance, and apart from certain topics in the field of neurophysiology such as vision, olfaction, learning and memory, honey bees are not a typical model system for studying general questions of insect physiology. The reason is their social lifestyle, which sets them apart from a "typical insect" and, during social evolution, has resulted in the restructuring of certain physiological pathways and biochemical characteristics in this insect. Not surprisingly, the questions that have attracted most attention by researchers working on honey bee physiology and biochemistry in general are core topics specifically related to social organization, such as caste development, reproductive division of labour and polyethism within the worker caste. With certain proteins playing key roles in these processes, such as the major royal jelly proteins (MRJPs), including royalactin and hexamerins in caste development, and vitellogenin in reproductive division of labour and age polyethism, a major section herein will present and discuss basic laboratory protocols for protein analyses established and standardized to address such questions in bees. A second major topic concerns endocrine mechanisms underlying processes of queen and worker development, as well as reproduction and polyethism, especially the roles of juvenile hormone and ecdysteroids. Sensitive techniques for the quantification of juvenile hormone levels circulating in haemolymph, as well as its synthesis by the corpora allata are described. Although these require certain instrumentation and a considerable degree of sophistication in the analysis procedures, we considered that presenting these techniques would be of interest to laboratories planning to specialize in such analyses. Since biogenic amines are both neurotransmitters and regulators of endocrine glands, we also present a standard method for the detection and analysis of certain biogenic amines of interest. Further questions that cross borders between individual and social physiology are related to energy metabolism and thermoregulation. Thus a further three sections are dedicated to protocols on carbohydrate quantification in body fluid, body temperature measurement and respirometry. Métodos estándar para la investigación de la fisiología y bioquímica de Apis mellifera ResumenA pesar de su enorme importancia económica, y aparte de ciertos temas en el campo de la neurofisiología, tales como la visión, el olfato, el aprendizaje y la memoria, las abejas no son un sistema modelo típico para el estudio de cuestiones generales sobre la fisiología de los insectos. La razón de ello es su forma de vida social, lo que las diferencia de un "insecto típico" y que durante la evolución social, se ha traducido en la reestructuración de ciertas vías fisiológicas y bioquímicas propias de este insecto. Como era de esperar, las preguntas que han atraído mayor atención por parte de los investigadores que trabajan en la fisiología y la bioquímica de la abeja melífera, son en general tema...

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Comparison of radioimmunoassay and liquid chromatography tandem mass spectrometry for determination of juvenile hormone titers

Cited by 26 publications

References 17 publications

Juvenile hormone titre and related gene expression during the change of reproductive modes in the pea aphid

Juvenile hormone titre and related gene expression during the change of reproductive modes in the pea aphid

Liquid chromatography coupled to ion trap-tandem mass spectrometry to evaluate juvenile hormone III levels in bee hemolymph from Nosema spp. infected colonies

Standard methods for physiology and biochemistry research inApis mellifera

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