Comparison of radioisotope methods and a nonradioisotope method to measure the RBC volume and RBC survival in the baboon

Abstract

The greater in vivo elution of 51Cr, 111In-oxine, and 99mTc than that of biotin-X-NHS influenced the measurements of RBC volume, 24-hour posttransfusion survival, and life span of the fresh baboon RBCs.

Posttransfusion 24‐hour recovery and subsequent survival of allogeneic red blood cells in the bloodstream of newborn infants

“…17 However, our MPL values are modestly shorter than those reported for stored human RBCs, which range from 103 to 116 days in adults, depending on the RBC-labeling method. 9,15,18,19 Because we performed a total of only eight studies (Table 1), the shorter MPL values may not reflect a genuine difference in posttransfusion survival compared with the results of others.…”

“…The difference may simply be a manifestation of the differences between results using biotinylated versus chromium-labeled RBCs. 17 Alternatively, because the posttransfusion survival studies reported by others were performed in adults, 9,15,18,19 our modestly shorter MPL values may be related to an undefined in-vivo factor unique to infants.…”

“…15,16 Our value of approximately 97 percent agrees with our earlier studies of normal adults 9 and with the value of 97 percent reported for baboons using fresh biotinylated RBCs. 17 Results with biotinylated RBCs are higher than those using chromium as the RBC label-mean values being approximately 83 percent for human chromium-labeled RBCs stored 42 days 15,16 and 92 percent for fresh baboon RBCs labeled with chromium. 17 We have no proven explanation for the apparent difference between results obtained using chromium versus biotin-labeling methods but speculate that, during the biotinylation procedure used to label RBCs for posttrans-fusion kinetic studies, those RBCs damaged during storage and destined to be removed from the bloodstream within the first 24 hours after transfusion are, instead, removed ex vivo by the multiple washing steps of the biotinylation process.…”

Plasmodium coatneyi in Rhesus Macaques Replicates the Multisystemic Dysfunction of Severe Malaria in Humans

“…Comparable procedures have been optimized in mice using in vivo biotinylation to quantify erythrocyte removal during the course of experimental infection with rodent malaria parasites (28) but not, to our knowledge, in naïve or malaria-infected nonhuman primates. Methodologies to quantify the erythrocyte life span in nonhuman primates have usually involved the autotransfusion of in vitro-labeled cells (29).…”

Effects of centrifugal and roller pumps on survival of autologous red cells in cardiopulmonary bypass surgery

“…Blood was collected 1 h after the bypass procedure, labeled with 51-Cr, and autotransfused to measure the 24-h post-transfusion survival. 7,8 …”