Comparison of reactive oxygen scavenging systems between a cetacean (DKN1) and a porcine renal epithelial cell line (LLC-PK1)

Pine, Michelle; Greer, Kimberly A.; Busbee, David L.

doi:10.1016/j.cbpa.2007.02.005

Cited by 4 publications

(4 citation statements)

References 27 publications

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“…In other cetacean species, a number of cell lines have been developed in order to assess viral impact (Cecil and Nigrelli, 1970, Nielsen et al, 1989, Kadoi et al, 1992 and the effects of contaminants (Wang and Pfeiffer, 2001, Fossi et al, 2006, Li Chen et al, 2009, Carvan et al, 1995, Spinsanti et al, 2008, Wise et al, 2010, Gauthier et al, 1999, Pine et al, 2007. Toxic effects in humpback whales are, however, still poorly understood and there are, to date, no established methods to study toxicological sensitivity.…”

Section: Introductionmentioning

confidence: 99%

Establishment of the first humpback whale fibroblast cell lines and their application in chemical risk assessment

et al. 2015

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This is a PDF file of an unedited manuscript that has been accepted for publication. As a service to our customers we are providing this early version of the manuscript. The manuscript will undergo copyediting, typesetting, and review of the resulting proof before it is published in its final form. Please note that during the production process errors may be discovered which could affect the content, and all legal disclaimers that apply to the journal pertain. HuWa1 exposed to a natural, p,p'-DDE-containing, chemical mixture extracted from whale blubber showed distinctively higher sensitivity than to p,p'-DDE alone. Thus, we provide the first cytotoxicological data for humpback whales and with establishment of the HuWa cell lines, a unique in vitro model for the study of the whales' sensitivity and cellular response to chemicals and other environmental stressors. Establishment

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Section: Introductionmentioning

confidence: 99%

Establishment of the first humpback whale fibroblast cell lines and their application in chemical risk assessment

et al. 2015

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“…Although further investigation is necessary to determine the underlying mechanism. Similar to a previous study, LLC-PK1 cells have been shown to be responsive to antioxidant treatments and are considered to be a well-established cell line for assessing antioxidant effects ( 18 ). In the present study, the effect of PPDE on LLC-PK1 cells was primarily investigated, and our future studies will focus on validating these findings and elucidating the underlying mechanisms in other cell lines.…”

Section: Discussionmentioning

confidence: 74%

Protective effect of Peucedanum praeruptorum Dunn extract on oxidative damage of LLC‑PK1 cells induced by H₂O₂

Hu,

Wang,

et al. 2023

Exp Ther Med

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Peucedanum praeruptorum Dunn extract (PPDE) is a well-known treatment used in traditional Chinese medicines, where it is most commonly used to treat coughs and symptoms such as headaches and fever. In the present study, the antioxidant capacity of PPDE in vitro was determined by scavenging experiments using DPPH, ABTS + ·, ·OH, and ·O 2 - . The cell survival rate was determined by MTT assay. The MDA, SOD, CAT, GSH, and GSH-Px content were determined by colorimetry assays. The expression levels of antioxidant genes SOD, CAT, GSH, and GSH-Px were assessed by reverse transcription-quantitative PCR. HPLC was used to identify the PPDE components. The results suggested that PPDE had scavenging effects on DPPH, ABTS, hydroxyl, and superoxide anion radicals in a concentration-dependent manner; H 2 O 2 treatment resulted in oxidative stress in LLC-PK1 cells, and the degree of injury of LLC-PK1 cells following PPDE treatment was improved, which was positively correlated with its concentration. Peucedanum praeruptorum Dunn extract treatment reduced the content of MDA and increased the content of CAT, SOD1, GSH, and GSH-Px. The mRNA expression levels of antioxidant genes detected by quantitative PCR were consistent with changes in CAT, SOD, GSS, and GSH-Px. Additionally, the trend in CAT, SOD1, GSH, and GSS protein expression levels was also consistent at the mRNA level. PPDE was found to consist of isochlorogenic acid C, myricetin, baicalin, luteolin, and kaempferol. Therefore, PPDE, which was formed of products derived from natural substances, functioned in the inhibition of oxidative damage. The present study aimed to obtain a better understanding of the traditional Chinese medicine Peucedanum praeruptorum Dunn and preliminarily elucidate its antioxidant mechanism at the cellular level. Further animal or human experiments are required to verify the antioxidant effects of PPDE for further development and utilization.

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“…Glutathione peroxidase is the most extensively studied selenoprotein for which protein functions and structure have been widely characterized and this protein has been identified in many animal species [4]. While GPx activity has been studied in tissues from bottlenose dolphins ( Tursiops truncatus ) [29, 30] and ringed seals ( Pusa hispida ) [31], this is the first study to separate and identify GPx1 at the protein level in a marine mammal species.…”

Section: Discussionmentioning

confidence: 99%

Selenium protein identification and profiling by mass spectrometry: A tool to assess progression of cardiomyopathy in a whale model

Bryan

Bossart²,

Christopher

et al. 2017

Journal of Trace Elements in Medicine and Biology

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Non-ischemic cardiomyopathy is a leading cause of congestive heart failure and sudden cardiac death in humans and in some cases the etiology of cardiomyopathy can include the downstream effects of an essential element deficiency. Of all mammal species, pygmy sperm whales (Kogia breviceps) present the greatest known prevalence of cardiomyopathy with more than half of examined individuals indicating the presence of cardiomyopathy from gross and histo-pathology. Several factors such as genetics, infectious agents, contaminants, biotoxins, and inappropriate dietary intake (vitamins, selenium, mercury, and pro-oxidants), may contribute to the development of idiopathic cardiomyopathy in K. breviceps. Due to the important role Se can play in antioxidant biochemistry and protein formation, Se protein presence and relative abundance were explored in cardiomyopathy related cases. Selenium proteins were separated and detected by multi-dimension liquid chromatography inductively coupled plasma mass spectrometry (LC-ICP-MS), Se protein identification was performed by liquid chromatography electrospray tandem mass spectrometry (LC-ESI-MS/MS), and Seprotein profiles were examined in liver (n = 30) and heart tissue (n = 5) by SEC/UV/ICP-MS detection. Data collected on selenium proteins was evaluated in the context of individual animal trace element concentration, life history, and histological information. Selenium containing protein peak profiles varied in presence and intensity between animals with no pathological findings of cardiomyopathy and animals exhibiting evidence of cardiomyopathy. In particular, one class of proteins, metallothioneins, was found to be associated with Se and was in greater abundance in animals with cardiomyopathy than those with no pathological findings. Profiling Se species with SEC/ICP-MS proved to be a useful tool to identify Se protein pattern differences between heart disease stages in K. breviceps and an approach similar to this may be applied to other species to study Se protein associations with cardiomyopathy.

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Comparison of reactive oxygen scavenging systems between a cetacean (DKN1) and a porcine renal epithelial cell line (LLC-PK1)

Cited by 4 publications

References 27 publications

Establishment of the first humpback whale fibroblast cell lines and their application in chemical risk assessment

Establishment of the first humpback whale fibroblast cell lines and their application in chemical risk assessment

Protective effect of Peucedanum praeruptorum Dunn extract on oxidative damage of LLC‑PK1 cells induced by H₂O₂

Selenium protein identification and profiling by mass spectrometry: A tool to assess progression of cardiomyopathy in a whale model

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Comparison of reactive oxygen scavenging systems between a cetacean (DKN1) and a porcine renal epithelial cell line (LLC-PK1)

Cited by 4 publications

References 27 publications

Establishment of the first humpback whale fibroblast cell lines and their application in chemical risk assessment

Establishment of the first humpback whale fibroblast cell lines and their application in chemical risk assessment

Protective effect of Peucedanum praeruptorum Dunn extract on oxidative damage of LLC‑PK1 cells induced by H2O2

Selenium protein identification and profiling by mass spectrometry: A tool to assess progression of cardiomyopathy in a whale model

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Protective effect of Peucedanum praeruptorum Dunn extract on oxidative damage of LLC‑PK1 cells induced by H₂O₂