Comparison of real-time PCR and conventional PCR for detection of Leishmania (Leishmania) infantum infection: a mini-review

Abstract

Abstract:In recent years, the polymerase chain reaction (PCR) technique has significantly advanced towards expanding its use and versatility by working with quantitative real-time PCR (qPCR). Data from the literature show that both methods present interesting characteristics for the diagnosis of visceral leishmaniasis. The benefits of qPCR in relation to conventional PCR include speed, reproducibility and quantitative ability. In addition to operational advantages, qPCR is more sensitive and reproducible and may replace conventional PCR in diagnostic routines. Regarding visceral leishmaniasis, the possibility of deployment of real-time PCR in highly complex diagnoses (reference services) in endemic areas will facilitate a swift and safe return for patients. Moreover, the use of a technique that possesses elevated diagnostic sensitivity, and can monitor therapy and prevent relapses promotes broader prospects for the disease control.

Comparative Value of Real Time and Conventional PCR in Antenatal Gender Determination

“…The operational advantages of real time PCR over conventional PCR are quantitative ability, speed, higher sensitivity and reproducibility [36].…”

Identification by real‐timePCRwithSYBRGreen ofLeishmaniaspp. andSerratia marcescensin canine ‘sterile’ cutaneous nodular lesions

“…6,7 Of the four Leishmaniapositive samples, three had a previous negative PCR for Leishmania spp. performed before their inclusion in the study; this might be related to the lower sensitivity of the direct PCR method in comparison to RT-PCR 14,15,33,34 and/or to inappropriate selection of paraffin-embedded material or of the probes. Our results provide further support that RT-PCR, as previously documented, 14,15 is superior to immunohistochemistry in detecting Leishmania spp.…”

Molecular Diagnosis of Leishmaniasis, Species Identification and Phylogenetic Analysis

“…qPCR is considered to be a helpful tool for Leishmania diagnosis, monitoring during therapy, development of new drugs and diagnostic tools, comparison of drug efficacy or prophylactic schemes, and for epidemiological studies. Regarding diagnosis of leishmaniasis, the kinetic study of parasitemia in the immunocompromised hosts, the diagnosis of relapses and the quantification of the low parasitic load in asymptomatic patients are of great interest [47].…”