Comparison of real-time PCR methods for quantification of European hake (Merluccius merluccius) in processed food samples

Sánchez, Arlex; Quinteiro, Javier; Vázquez, Jesús; Pérez‐Martín, Ricardo I.; Sotelo, Carmen G.

doi:10.1016/j.foodchem.2018.08.031

Cited by 5 publications

(6 citation statements)

References 30 publications

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“…In the literature, there is a large number of methods to quantify material of animal or plant origin in food products [13,41]. Some of these methods are not suited for processed food products, and were therefore not considered in this study.…”

Section: Discussionmentioning

confidence: 99%

Comparison of Real-Time PCR Quantification Methods in the Identification of Poultry Species in Meat Products

Dolch

Andrée

Schwägele

2020

Foods

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Poultry meat is consumed worldwide and is prone to food fraud because of large price differences among meat from different poultry species. Precise and sensitive analytical methods are necessary to control poultry meat products. We chose species–specific sequences of the cytochrome b gene to develop two multiplex real-time polymerase chain reaction (real-time PCR) systems: one for chicken (Gallus gallus), guinea fowl (Numida meleagris), and pheasant (Phasianus colchicus), and one for quail (Coturnix japonica) and turkey (Meleagris gallopavo). For each species, added meat could be detected down to 0.5 % w/w. No cross reactions were seen. For these two real-time PCR systems, we applied three different quantification methods: (A) with relative standard curves, (B) with matrix-specific multiplication factors, and (C) with an internal DNA reference sequence to normalize and to control inhibition. All three quantification methods had reasonable recovery rates from 43% to 173%. Method B had more accepted recovery rates, i.e., in the range 70–130%, namely 83% compared to 75% for method A or C.

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Section: Discussionmentioning

confidence: 99%

Comparison of Real-Time PCR Quantification Methods in the Identification of Poultry Species in Meat Products

Dolch

Andrée

Schwägele

2020

Foods

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“…Sánchez A. et al [40] describe the use of real-time PCR for absolute, absolute-relative and relative quantification of the most valued hake species in European markets, European hake (Merluccius merluccius). The authors compared two systems, nuclear and mitochondrial.…”

Section: Real-time Pcrmentioning

confidence: 99%

“…The research showed that the best quantification results for this species in binary mixtures with non-target species (Merluccius capensis) and using the species-specific realtime PCR system were achieved with a relative quantification approach. It was demonstrated that absolute quantification using the nuclear system was appropriate for the quantification of the Merluccius genus in food model samples [40].…”

Section: Real-time Pcrmentioning

confidence: 99%

Methods of Molecular Diagnostics for Fish Species Identification

2020

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The growth in demand for fish products as a result of globalization of trade caused a risks and threats of selling poor-quality and falsified fish products. This has become a great problem both for supervising agencies and for consumers.Many countries have regulations on food labelling and safety. For example, in the Russian Federation, Republic of Belarus and Republic of Kazakhstan has been passed the Technical Regulation of the Customs Union TR CU022/2011 “Food products in part of their labeling” that aims to prevent misinformation of consumers to ensuring realization of consumer rights to reliable information about food products, and Technical Regulation TR EAEU040/2016 “On safety of fish and fish products” requires indication of the zoological name of the species of the aquatic biological resource or the object of aquaculture.Fish species identification is traditionally carried out based on external morphological traits. However, it becomes impossible to identify species by ichthyological traits upon fish cutting, if the head and fins are removed, and the body is cut on pieces (especially, in case of fillets) and even more so upon technological processing. In this case, objective analytical methods of species identification are used, which are based on ELISA or PCR. However, DNA‑based methods have several advantages compared to ELISA methods and complement traditional morphological identification methods. This paper gives a wide overview of the most recent and used methods of fish species identification based on DNA analysis such as single-strand conformation polymorphism (SSCP) analysis, species-specific PCR, real-time PCR, polymerase chain reaction-restriction fragment length polymorphism analysis (PCR-RFLP), DNA barcoding, Sanger sequencing and next-generation sequencing (NGS).

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“…Floren et al, 2015;Thomas et al, 2016;Ushio et al, 2018). Quantifying input biomass from DNA analysed in fish products is an emerging area of research and has a large unexplored potential in comparison to traditional visual identification, especially in samples where the morphological characteristics are sparse or absent (Nagase et al, 2010;Bojolly et al, 2017;Sánchez et al, 2019). Further, DNA, particularly mitochondrial DNA (mtDNA), has been found suitable for species detection and quantification even when products are highly processed (e.g.…”

Section: Introductionmentioning

confidence: 99%

From DNA to biomass: opportunities and challenges in species quantification of bulk fisheries products

Hansen

Farrant

Ogden

et al. 2020

ICES Journal of Marine Science

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Fisheries enforcement relies on visual catch identification and quantification at sea or when landed. Silage (fish dissolved in acid) and fish blocks (block frozen fish) are promising methods for on-board processing and storage of low-value catches. We examined the use of non-destructive sampling and two DNA-based methods, quantitative PCR (qPCR) and metabarcoding, to assess species composition and relative abundance in industrial grade experimental silage and fish blocks. We demonstrate the ability to identify and quantify DNA from fish species in both products. qPCR analysis of small silage samples collected over 21 days detected all target control species. DNA from one species (Atlantic wolffish) was consistently overrepresented while, for three species of gadoids (Atlantic cod, haddock and whiting), the DNA content matched input tissue proportions with high accuracy. qPCR and metabarcoding of fish blocks, sampled as run-off water and exterior swabs, provided consistent species detection, with the highest variance observed in quantification from swab samples. Our analysis shows that DNA-based methods have significant potential as a tool for species identification and quantification of complex on-board-processed seafood products and are readily applicable to taxonomically and morphologically similar fish. There is, however, a need for establishing DNA/weight calibration factors for primary fisheries species.

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Comparison of real-time PCR methods for quantification of European hake (Merluccius merluccius) in processed food samples

Cited by 5 publications

References 30 publications

Comparison of Real-Time PCR Quantification Methods in the Identification of Poultry Species in Meat Products

Comparison of Real-Time PCR Quantification Methods in the Identification of Poultry Species in Meat Products

Methods of Molecular Diagnostics for Fish Species Identification

From DNA to biomass: opportunities and challenges in species quantification of bulk fisheries products

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