Comparison of real-time polymerase chain reaction and DNA-strip technology in microbiological evaluation of periodontitis treatment

Evaluation of a novel immunochromatographic device for rapid and accurate clinical detection of Porphyromonas gingivalis in subgingival plaque

“…The clinical detection threshold of the current device was estimated to be approximately 10 4 copies/two paper points, based on our preliminary experiments and the results from this clinical application. This threshold is comparable with those reported for a DNA probe method (Komiya et al, 2000), an antibody-based detection test (Nakagawa et al, 1995;Boyer et al, 1996) and a DNA-strip technology-based method (Eick et al, 2011), and is also considered to be clinically relevant because such a threshold potentially matches the range in which nonsurgical or surgical therapy is unsuccessful and antimicrobial treatment is needed (Preshaw, 2004;Kaman et al, 2012).…”

“…Microbiological assessment of periodontal bacteria is important in selecting appropriate antimicrobial therapy, in deciding the need for periodontal surgery, and in evaluating the treatment outcome (Teles et al, 2006;Xiang et al, 2010). For clinicians in private practice settings, bacterial detection services such as those using real-time polymerase chain reaction (RT-PCR) method and test system using DNA-strip technology (Eick et al, 2011) are commercially available. They are, however, costly and not considered to be point-of-care testing per se.…”

Multiplex real‐time PCR detection and relative quantification of periodontal pathogens

“…A variety of techniques have been used for examining the microbial composition in periodontal clinical samples (Eick et al, 2011; Rocas et al, 2015; Zambon & Haraszthy, 1995). These assessments have been invaluable to dental basic science and translational research.…”

Microbiologic Findings in Relation to Risk Assessment for Periodontal Disease: A Cross‐Sectional Study

“…23 Eleven bacteria previously considered associated with periodontal disease (Aggregatibacter actinomycetemcomitans, Porphyromonas gingivalis, Tanerella forsythia, Treponema denticola, Prevotella intermedia, Parvimonas micra, Fusobacterium nucleatum, Campylobacter rectus, Eubacterium nodatum, Eikenella corrodens, and Capnocytophaga spec.) were tested using this probe from individual pooled samples of subgingival plaque obtained from the deepest pocket in each sextant.…”