Comparison of Recombinant Barramundi and Human Insulin-like Growth Factor (IGF)-I in Juvenile Barramundi (Lates calcarifer): In Vivo Metabolic Effects, Association with Circulating IGF-Binding Proteins, and Tissue Localisation

Degger, Brian; Upton, Zee; Soole, Kathleen L.; Collet, Christopher; Richardson, Neil

doi:10.1006/gcen.1999.7417

Cited by 42 publications

(22 citation statements)

References 33 publications

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“…No study of piscine systems has been able to demonstrate the presence of a ternary complex of IGF-I, 41-kDa IGFBP, and ALS (9,40,46). ALS is a relatively large protein that greatly retards clearance of IGF-I and IGFBP-3 in mammals.…”

Section: Discussionmentioning

confidence: 99%

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Postprandial changes in plasma growth hormone, insulin, insulin-like growth factor (IGF)-I, and IGF-binding proteins in coho salmon fasted for varying periods

Shimizu¹,

Cooper²,

Dickhoff³

et al. 2009

American Journal of Physiology-Regulatory, Integrative and Comp

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Fish were initially fasted for 1 day, 1 wk, or 3 wk. Fasted fish were then fed, and blood was collected at 4-h intervals over 26 h. After the various periods of fasting, basal levels of insulin were relatively constant, whereas those of IGF-I, IGFBPs and GH changed in proportion to the duration of the fast. A single meal caused a rapid, large increase in the circulating insulin levels, but the degree of the increase was influenced by the fasting period. IGF-I showed a moderate increase 2 h after the meal but only in the regularly fed fish. Plasma levels of 41-kDa IGFBP were increased in all groups within 6 h after the single meal. The fasting period did not influence the response of 41-kDa IGFBP to the meal. IGFBP-1 and GH decreased after the meal to the same extent among groups regardless of the fasting period. The present study shows that insulin and IGF-I respond differently to long (weeks)-and short (hours)-term nutritional changes in salmon; insulin maintains its basal level but changes acutely in response to food intake, whereas IGF-I adjusts its basal levels to the long-term nutritional status and is less responsive to acute nutritional input. IGFBPs maintain their sensitivity to food intake, even after prolonged fasting, suggesting their critical role in the nutritional regulation of salmon growth.fasting; refeeding; growth; metabolism GROWTH OCCURS WHEN THERE IS a net positive difference between the anabolic and catabolic processes of metabolism. Metabolism rapidly shifts between anabolic and catabolic processes on the order of minutes to hours, depending on current energy intake and expenditures. Growth varies at longer time scales of days to weeks and even months, depending on net energy intake averaged over these same days and weeks. Metabolism directly responds to current stimuli, while changes in growth rate are due not only to current intake but also to nutrient reserves stored over time. Thus growth is integrally connected to metabolism.Regulation of growth and metabolism is among the primary functions of the endocrine system. Both growth and metabolism are mediated and modulated through a myriad of differing factors that include integration and stimulation through the hypothalamic-pituitary axis; however, some primary mediators have been identified in the peripheral circulation (23). Pancreatic hormones such as insulin and glucagon are important regulators of anabolic and catabolic processes, respectively (24, 33). Growth is mediated through the actions of growth hormone (GH)-insulin-like growth factor (IGF)-I system. Interestingly, insulin and IGF-I are structurally related peptides, which arose from gene duplications before the emergence of the Chondrichthyes (8). Endocrine mechanisms have developed such that these structurally similar hormones modulate differing yet linked aspects of the physiology of metabolism and growth.The kinetics of insulin and total IGF-I in the circulation are markedly different. Circulating insulin shows a rapid change in response to a meal and its half-life in the c...

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Section: Discussionmentioning

confidence: 99%

“…Circulating insulin shows a rapid change in response to a meal and its half-life in the circulation is less than 5 min (6). In contrast, total IGF-I level is relatively stable, and its half-life is ϳ12 h in humans and fish (9,15). This difference is attributed, at least partly, to the presence of a family of six IGF-binding proteins (IGFBPs).…”

mentioning

confidence: 99%

Postprandial changes in plasma growth hormone, insulin, insulin-like growth factor (IGF)-I, and IGF-binding proteins in coho salmon fasted for varying periods

Shimizu¹,

Cooper²,

Dickhoff³

et al. 2009

American Journal of Physiology-Regulatory, Integrative and Comp

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show abstract

“…This ratio has been observed during trout ontogeny and in adult fish muscle (38) and also in a primary culture of rainbow trout muscle cells (5), but at the present time there is a lack of evidence on the role of IGF-I in fish muscle. Drakenberg et al (14) and Degger et al (11) showed that IGF-I in vivo administration increased glucose incorporation to fish muscle glycogen. Negatu and Meier (42) and Degger et al (11) reported that IGF-I and insulin stimulate amino acid uptake in this tissue in Gulf killifish and in barramundi.…”

mentioning

confidence: 96%

“…Drakenberg et al (14) and Degger et al (11) showed that IGF-I in vivo administration increased glucose incorporation to fish muscle glycogen. Negatu and Meier (42) and Degger et al (11) reported that IGF-I and insulin stimulate amino acid uptake in this tissue in Gulf killifish and in barramundi.…”

mentioning

confidence: 96%

Metabolic and mitogenic effects of IGF-I and insulin on muscle cells of rainbow trout

Castillo

Codina

Martínez

et al. 2004

American Journal of Physiology-Regulatory, Integrative and Comp

141

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The relative function of IGF-I and insulin on fish muscle metabolism and growth has been investigated by the isolation and culture at different stages (myoblasts at day 1, myocytes at day 4, and myotubes at day 10) of rainbow trout muscle cells. This in vitro model avoids interactions with endogenous peptides, which could interfere with the muscle response. In these cells, the effects of IGF-I and insulin on cell proliferation, 2-deoxyglucose (2-DG), and l-alanine uptake at different development stages, and the use of inhibitors were studied and quantified. Insulin (10-1,000 nM) and IGF-I (10-100 nM) stimulated 2-DG uptake in trout myocytes at day 4 in a similar manner (maximum of 124% for insulin and of 142% for IGF-I), and this stimulation increased when cells differentiated to myotubes (maximum for IGF-I of 193%). When incubating the cells with PD-98059 and especially cytochalasin B, a reduction in 2-DG uptake was observed, suggesting that glucose transport takes place through specific facilitative transporters. IGF-I (1-100 nM) stimulated the l-alanine uptake in myocytes at day 4 (maximum of 239%), reaching higher values of stimulation than insulin (100-1,000 nM) (maximum of 160%). This stimulation decreased when cells developed to myotubes at day 10 (118% for IGF-I and 114% for insulin). IGF-I (0.125-25 nM) had a significant effect on myoblast proliferation, measured by thymidine incorporation (maximum of 170%), and required the presence of 2-5% fetal serum (FBS) to promote thymidine uptake. On the other hand, insulin was totally ineffective in stimulating thymidine uptake. We conclude that IGF-I is more effective than insulin in stimulating glucose and alanine uptake in rainbow trout myosatellite cells and that the degree of stimulation changes when cells differentiate to myotubes. IGF-I stimulates cell proliferation in this model of muscle in vitro and insulin does not. These results indicate the important role of IGF-I on growth and metabolism of fish muscle.

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“…In vertebrates, the insulin-like growth factor (IGF) system regulates this process and is composed of the insulin-like growth factors (IGFs; IGF-1 and IGF-2), the IGF receptors (IGF-1R and IGF-2R), and several IGF binding proteins (IGFBPs) (Moriyama et al, 2000;Duan et al, 2010;Johnston et al, 2011;Fuentes et al, 2013b). In fish, IGF-1 is the key hormone regulating skeletal muscle growth by stimulating the proliferation and differentiation of myogenic cells as well as protein synthesis (Degger et al, 2000;Castillo et al, 2006;Garikipati and Rodgers, 2012a,b;Fuentes et al, 2013b).…”

Section: Introductionmentioning

confidence: 99%

The TORC1/P70S6K and TORC1/4EBP1 signaling pathways have a stronger contribution on skeletal muscle growth than MAPK/ERK in an early vertebrate: Differential involvement of the IGF system and atrogenes

Fuentes

Einarsdóttir

Paredes

et al. 2015

General and Comparative Endocrinology

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Comparison of Recombinant Barramundi and Human Insulin-like Growth Factor (IGF)-I in Juvenile Barramundi (Lates calcarifer): In Vivo Metabolic Effects, Association with Circulating IGF-Binding Proteins, and Tissue Localisation

Cited by 42 publications

References 33 publications

Postprandial changes in plasma growth hormone, insulin, insulin-like growth factor (IGF)-I, and IGF-binding proteins in coho salmon fasted for varying periods

Postprandial changes in plasma growth hormone, insulin, insulin-like growth factor (IGF)-I, and IGF-binding proteins in coho salmon fasted for varying periods

Metabolic and mitogenic effects of IGF-I and insulin on muscle cells of rainbow trout

The TORC1/P70S6K and TORC1/4EBP1 signaling pathways have a stronger contribution on skeletal muscle growth than MAPK/ERK in an early vertebrate: Differential involvement of the IGF system and atrogenes

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