Comparison of regression for blood ALP levels using methods of the Japan Society of Clinical Chemistry and the International Federation of Clinical Chemistry and Laboratory Medicine in bovine, canine, feline, and human testing

Abstract

Livestock and companion animal health have a direct impact on human health. Research on clinical laboratory technology for veterinary medicine is as important as that on human laboratory technology. Reagents and analysis equipment for human medical laboratory tests are often used in veterinary medicine. Medical laboratories in Japan utilize the Japan Society of Clinical Chemistry (JSCC) method for blood alkaline phosphatase (ALP) analysis. The International Federation of Clinical Chemistry and Laboratory Medicine (IFCC) method is used worldwide for ALP catalytic concentration measurement. When the IFCC method is used, human blood ALP activity is approximately one-third of the JSCC method’s activity. The JSCC method for ALP measurement was switched to the IFCC method in medical laboratories in Japan in April 2020 for global standardization purpose. It is uncertain whether conventional JSCC method reagents will continue to be supplied. In veterinary medicine, the relationship between the JSCC and IFCC methods in terms of ALP measurement is almost unclear. This study investigated the regression between JSCC and IFCC methods measuring ALP in bovine, canine, feline, and human. The regression formulas for bovine, canine, feline, and human ALP values using the conventional JSCC (x) and IFCC (y) methods are y = 0.379x + 0.124, y = 0.289x + 8.291, y = 0.358x + 0.432, and y = 0.337x + 2.959, respectively. These results suggested that the IFCC method measurement could be estimated by approximately one-third of the JSCC method measurement in animal species such as bovine, canine, and feline. By applying the conversion factors proposed in this study, a very good correlation could be obtained between the two methods for each animal.

Changes in the liver transcriptome and physiological parameters of Japanese Black steers during the fattening period

“…These metabolites are present in hepatocytes and released into the blood stream when hepatocytes are damaged by stimulation such as mold toxins and long-term high-energy feeding. Blood ALP—mainly released from the liver and bones—is related to liver dysfunction and bone development in beef cattle 22 , 23 . In growing animals, blood ALP is mainly released from bone tissue; the main tissue is changed from bone to the liver and the overall release decreases with growth 24 , 25 .…”

Prepartum measurement of serum biomarkers reflecting osteoclastic and osteoblastic bone metabolism for predicting the risk of milk fever in dairy cows

“…Starting in April 2020, medical laboratories in Japan have been switching from the JSCC method to the International Federation of Clinical Chemistry and Laboratory Medicine (IFCC) method for measuring t-ALP. The IFCC reagents contain a 2-amino-2-methyl-1-propanol buffer instead of EAE buffer (Hata et al ., 2021). Because the IFCC method is currently used worldwide for t-ALP measurement, it is necessary to accumulate the ALP3 data by the IFCC method.…”

“…After electrophoresis (23 min at 230 V and 15°C), the gels were stained and scanned as densitometric images. The ALP3 fraction (%) was assessed; its activity was calculated from the total ALP (t-ALP) activity measured using the biochemical autoanalyser (TBA-120FR) with the Japan Society of Clinical Chemistry (JSCC) method reagent kit (ALP-II Seiken; Denka Seiken) containing a 4-nitrophenyl phosphate disodium salt hexahydrate (pNPP) substrate and a 2-ethylaminoethanol (EAE) buffer (Hata et al ., 2021). The serum O/T ratio was calculated for each animal to assess the OPG activity acting on a single osteoclast (Hatate et al ., 2020).…”

Changes in The Liver Transcriptome and Physiological Parameters of Japanese Black Steers During The Fattening Period

“…These metabolites are present in hepatocytes and released into the blood stream when hepatocytes are damaged by stimulation such as mold toxins and longterm high-energy feeding. Blood ALP-mainly released from the liver and bones-is related to liver dysfunction and bone development in beef cattle 21,22 . In growing animals, blood ALP is mainly released from bone tissue; the main tissue that releases the ALP into the blood stream transforms into liver tissue and the overall release decreases with growth 23,24 .…”