Comparison of relative binding affinities of endocrine active compounds to fathead minnow and rainbow trout estrogen receptors

Denny, Jeffrey S.; Tapper, Mark A.; Schmieder, Patricia K.; Hornung, Michael W.; Jensen, Kathleen; Ankley, Gerald T.; Henry, Tala R.

doi:10.1897/04-595r.1

Cited by 60 publications

(45 citation statements)

References 43 publications

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“…Finally, sample size for some of the treatments were low (n = 3), and therefore there was a greater chance for variability. The rationale for using this mixture was that NP and OP and their ethoxylates have been shown to induce VTG mRNA of juvenile and male animals in a number of fish species, and analytical standards to measure these compounds in environmental media were available (Denny et al 2005;Ishibashi et al 2006;Li et al 2012;Jobling and Sumpter 1993;Schoenfuss et al 2008;Van den Belt et al 2003;Ferguson et al 2003). In contrast, the study by Xie et al (2005) used an unknown mixture of AP and APEs in a commercial product (TPA) that contained a multitude of ethoxylate and alkylphenol isomers, which have different estrogenic potencies as well as targets exclusive of the estrogen receptor (Nimrod and Benson 1996;Laws et al 2000;Yadetie et al 1999).…”

Section: Discussionmentioning

confidence: 99%

Exploring the Impacts of Two Separate Mixtures of Pesticide and Surfactants on Estrogenic Activity in Male Fathead Minnows and Rainbow Trout

Crago

Tran

Budicin

et al. 2014

Arch Environ Contam Toxicol

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In this study, male fathead minnows (FHM) (Pimephales promelas) and juvenile rainbow trout (RT; Oncorhynchus mykiss) were exposed to two different surfactant mixtures of analytical-grade nonlyphenol, 4-tert octyphenol, octylphenol ethoxylates, nonylphenol ethoxylates, and the herbicide 2,4-dichlorophenoxyacetic acid (2,4-D). After a 7-days exposure to environmentally relevant concentrations of these compounds, there was no difference in the relative mRNA expression of vitellogenin (VTG) in male juvenile RT exposed to individual compounds or the 2,4-D-surfactant mixture compared with the control. In male FHM, there was a significant increase in VTG mRNA expression in the high individual treatments of 2,4-D and the surfactants but not the 2,4-D-surfactant mixtures compared with the control. These results were compared with another study exposing male FHM to individual and a mixture of alkylphenols and alkylphenol ethoxylates in two different combinations with the herbicide diuron and the insecticide bifenthrin. There were no differences in the relative expression of VTG mRNA amongst individual exposures and the control. Interestingly, when the ethoxylate mixture was combined with diuron, there was a significant decrease in the relative mRNA expression of VTG compared with the control. However, when the ethoxylate mixture was combined with both diuron and bifenthrin, there was a significant increase in the relative mRNA expression of VTG in male compared with all other groups in the multichemical mixture. The results of this study highlight differences between species and measurements of VTG in assessing the risk of mixtures to aquatic organisms.

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Section: Discussionmentioning

confidence: 99%

Exploring the Impacts of Two Separate Mixtures of Pesticide and Surfactants on Estrogenic Activity in Male Fathead Minnows and Rainbow Trout

Crago

Tran

Budicin

et al. 2014

Arch Environ Contam Toxicol

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“…It was found that higher concentrations of E3 (1.62-9.55 nM) were necessary for the transcription of fish ERα compared to the induction by E2 (0.18-0.36 nM). Several studies have determined the estrogenic effects of E3 using in vitro assays (Tollefsen et al, 2003;Denny et al, 2005;Luo et al, 2011;Lange et al, 2012). However, few studies reported the estrogenic effect of E3 on fish using in vivo assays, and in these studies, only early life stage of fish was exposed to E3, or only single test endpoint such as intersex (i.e., testis-ova) in fish was investigated (Metcalfe et al, 2001;Holbech et al, 2006).…”

Section: Introductionmentioning

confidence: 99%

Long-term exposure investigating the estrogenic potency of estriol in Japanese medaka (Oryzias latipes)

Lei

Kang

et al. 2014

Comparative Biochemistry and Physiology Part C: Toxicology &

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The growth, development, and ERα and Vtg-I gene expressions of Japanese ricefish (Oryzias latipes; medaka) exposed to different concentrations of estriol (E3), including one environmentally relevant concentration, during embryo-adult life stages were evaluated. At the early life stage, fertilized eggs were exposed to 5, 50, 500, 5000 ng/L E3 for 15 days, and the hatched fry were exposed continuously to the same concentrations for an additional 15 days. Exposure to 500 and 5000 ng/L E3 resulted in adverse effects on hatchability and time to hatching. At 5000 ng/L, the gross abnormality rate was increased and the number of females that hatched was twice that of males. When the fish were exposed to 5-5000 ng/L E3 for further 60 days, the male hepatosomatic index (HSI) was increased at 5000 ng/L. The female gonadosomatic index (GSI) was decreased at 500 and 5000 ng/L E3, while the male GSI at 5000 ng/L E3 was increased and sex reversal was also found at this concentration. Quantitative RT-PCR showed that the hepatic vitellogenin-I (Vtg-I) genes were up-regulated in females at 500 and 5000 ng/L E3 and in males at all E3 concentrations, whereas E3 did not affect estrogen receptor α (ERα) mRNA transcription. These results showed that E3 at environmental concentration of 5 ng/L has no adverse effects on growth and development of the Japanese medaka. However, in this study, if we only focused on Vtg gene change in males, E3 had strong estrogenic effects on male medaka under the conditions of these experiments.

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“…These compounds include both environmental contaminants with binding affinities for the estrogen or androgen receptors, such as nonylphenol, methyoxychlor and trenbelone (Denny et al, 2005,Ankley et al, 2003,Larkin et al, 2002a, as well as human and veterinary pharmaceuticals released into the environment, including ethynyl estradiol (EE2) and trenbelone (Kolodziej et al, 2004). The contributions of these compounds towards a suite of disorders in humans, including debate over decreased male fertility due to reduced sperm counts and increased rates of breast cancers (Safe, 1995).…”

Section: Introductionmentioning

confidence: 99%

Temporal changes in gene expression in rainbow trout exposed to ethynyl estradiol

Hook

Skillman

Small

et al. 2007

Comparative Biochemistry and Physiology Part C: Toxicology &

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We examined changes in the genomic response during continuous exposure to the xenoestrogen ethynylestradiol. Isogenic rainbow trout Oncorhynchus mykiss were exposed to nominal concentrations of 100 ng/L ethynyl estradiol (EE2) for a period of three weeks. At fixed time points within the exposure, fish were euthanized, livers harvested and RNA extracted. Fluorescently labeled cDNA were generated and hybridized against a commercially available Salmonid array (GRASP project, University of Victoria, Canada) spotted with 16,000 cDNAs. The slides were scanned to measure abundance of a given transcript in each sample relative to controls. Data were analyzed via Genespring (Silicon Genetics) to identify a list of up and down regulated genes, and to determine gene clustering patterns that can be used as "expression signatures". Gene ontology was determined using the annotation available from the GRASP website. Our analysis indicates each exposure time period generated specific gene expression profiles. Changes in gene expression were best understood by grouping genes by their gene expression profiles rather than examining fold change at a particular time point. Many of the genes commonly used as biomarkers of exposure to xenoestrogens were not induced initially and did not have gene expression profiles typical of the majority of genes with altered expression.

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Comparison of relative binding affinities of endocrine active compounds to fathead minnow and rainbow trout estrogen receptors

Cited by 60 publications

References 43 publications

Exploring the Impacts of Two Separate Mixtures of Pesticide and Surfactants on Estrogenic Activity in Male Fathead Minnows and Rainbow Trout

Exploring the Impacts of Two Separate Mixtures of Pesticide and Surfactants on Estrogenic Activity in Male Fathead Minnows and Rainbow Trout

Long-term exposure investigating the estrogenic potency of estriol in Japanese medaka (Oryzias latipes)

Temporal changes in gene expression in rainbow trout exposed to ethynyl estradiol

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