Comparison of rheumatoid arthritis (RA) and osteoarthritis (OA) based on microarray profiles of human joint fibroblast‐like synoviocytes

Abstract

The purpose of the present study was to investigate the underlying molecular mechanism of osteoarthritis (OA) and rheumatoid arthritis (RA) based on microarray profiles. Three human joint fibroblast-like synoviocytes (FLSs) microarray profiles including 26 OA samples, 33 RA samples, and 20 healthy control (HC) samples were downloaded from the GEO database. Differentially expressed genes (DEGs) between OA and HC (DEGsOA) and RA and HC (DEGsRA) were identified. Co-expressed and specific genes were analysed between DEGsOA and DEGsRA. Gene ontology, KEGG pathway enrichment, PPI network, and GSEA were performed to predict the function of DEGs. Two hundred seventy-six and 410 differential genes in DEGsOA and DEGsRA were observed. One hundred fifty coexpressed genes and 126 OA-specific genes (SELE, SERPINE1, and NFKBIA were the key genes) between DEGsOA and DEGsRA were enriched in the tumour necrosis factor (TNF) signalling pathway. However, 260 RA-specific genes of which the key genes were CCR5, CCR7, CXCR4, CCL5, and CCR4 were enriched in chemokine signalling pathway. Therefore, FLSs might exert an inflammatory effect by regulating TNF signalling pathway, targeting SELE, SERPINE1, and NFKBIA during the process of OA. Although TNF signalling pathway was also involved in the synovitis of RA, chemokine signalling pathway played the key role in RA FLSs mediating cell migration, invasion, and release of chemotaxis. In addition, CCR5, CCR7, CXCR4, CCL5, and CCR4 might be hub genes in RA. The different biomarkers and pathways identified in OA and RA may provide references for further study.List of abbreviations: CCL5, C-C motif chemokine ligand 5; CCRs, C-C motif chemokine receptors; CD95, FAS cell surface death receptor; Co-expressed genes, the common genes both in DEGsOA and DEGsRA groups; CXCR4, C-X-C motif chemokine receptor 4; DAVID, Database for Annotation, Visualization, and Integrated Discovery; DEGs, differently expressed genes; DEGsOA, differently expressed genes between osteoarthritis and healthy fibroblast-like synoviocytes; DEGsRA, differently expressed genes between rheumatoid arthritis and healthy fibroblast-like synoviocytes; DLL1, delta-like protein 1

Syndecan‐4 involves in the pathogenesis of rheumatoid arthritis by regulating the inflammatory response and apoptosis of fibroblast‐like synoviocytes

“…The top 10 enriched pathways (ranged by Count) are shown in Figure a. As expected, the top three pathways (cytokine receptor interaction, chemokine signaling pathway, and cell adhesion molecular [CAMs]) all indicated that the interaction between cytokines/chemokines and their receptor on plasma membrane might be the main pathogenesis of RA (Cai et al, ; Mori et al, ), consistent with the results of GO analysis (Figure c). In the PPI network of DEGs, the top 5 hub genes including CD44, C‐X‐C motif chemokine ligand 10 (CXCL10), SDC4, guanylate binding protein 1 (GBP1), and MMP1 were identified (Figure b).…”

Identification of potential specific biomarkers and key signaling pathways between osteogenic and adipogenic differentiation of hBMSCs for osteoporosis therapy

“…DEGs were identified by the inclusion criteria with P value < 0.05 and fold change (FC) ≥ 2 [ 25 ]. The visualization of DEGs was drawn via volcano plot and Venn diagram by using the ggplot2 and VennDiagram packages in R language (version 3.5.1) ( http://www.r-project.org/ ) [ 26 ].…”

Identification of potential specific biomarkers and key signaling pathways between osteogenic and adipogenic differentiation of hBMSCs for osteoporosis therapy

“…DEGs were identi ed by the inclusion criteria with P. value <0.05 and fold change (FC) ≥ 2 [25]. The visualization of DEGs was drawn via volcano plot and venn diagram by using the ggplot2 and VennDiagram packages in R language (version 3.5.1) (http://www.r-project.org/) [26].…”