Background: The relationship between stroke and mineral status is inconsistent. With regard to doubt in the role of mineral, this study was conducted. Material and Method: This cross-sectional study on 216 stroke patients who referred to the Ayatollah Rouhani Hospital of Babol over one-year period were conducted. Serum level of calcium (Ca), magnesium (Mg), Vitamin D (VD) were measured. Chi-square or Fisher exact test in spss 23 used. Result: Difference of hemorrhagic subtype was statistically significant in hypertensive patients (20(87%) ICH vs. 1(12.5%) SAH, p = 0.001). In thrombotic patients, 98 cases (89%) low VD vs. 12 cases (10.9%) normal VD, (p = 0.001) were seen. Also, low VD was more seen significantly in DM patients 72(90%) in compare to non-DM patients 96(72.7%), (p = 0.003). Of 120 cases (56.3%) who had HTN, 103 cases (85.8%) low VD vs. 17 cases (14.2%) normal VD, (p = 0.01), 68 cases (57.1%) hypo calcemic vs. 45 cases (37.8%) normal Ca serum level, (p < 0.0001) and 57 cases (49.1%) hypomagnesemia vs. 59 cases (50.9%) normal magnesium (p = 0.04) were seen. Difference in sex was statistically significant with hypomagnesemia (49 cases (53.8%) male vs. 38 cases (33.9%) female, p = 0.007). Hypomagnesemia were seen in 87 cases (40.8%) that 49 cases of this were hypo-calcemic (56.3%), (p = 0.03). Differences in magnesium with VD was statistically significant (170 cases (79.4%) low VD vs. 44 cases (20.6%) normal, p = 0.007). Conclusion: Low VD in thrombotic and diabetic patients, low Ca and VD in hypertensive patients and hypocalcemia were more seen in hypermagnesemic patients. Preventive policies such as screening of mineral serum level are suggested.