Comparison of SDS–PAGE protein patterns with other typing methods for investigating the epidemiology of ‘Klebsiella aerogenes’

Abstract

SUMMARYTwenty-four cultures comprising 20 clinical isolates of 'Klebsiella aerogenes' from two hospitals, a reference strain of 'K. aerogenes' and the type strains of three other Klebsiella species, were characterized by one-dimensional sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE) of whole-cell proteins. The protein patterns were highly reproducible and were used as the basis of a numerical analysis which divided the clinical isolates into 12 protein types. Comparison with established typing methods indicated that the level of discrimination of SDS-PAGE was similar to that achieved with conventional typing methods but the strains were grouped differently. Protein typing subdivided five serotype K3 isolates that could also be distinguished by phage typing. Conversely, three strains of protein type 11 were clearly distinguishable by both serotyping and phage typing. We conclude that high-resolution SDS-PAGE of proteins provides an effective adjunct to other methods for typing isolates of 'K. aerogenes'.

Thirty Years of Numerical Taxonomy

“…Two contrasting studies show its power. In the first (Costas et al, 1990a), cross-infection from patient to patient was confirmed; the strains from patients in one hospital ward were highly similar and formed a tight cluster, unlike strains from other wards. In the second (Costas et al, 1990b), the outbreak was from a common source, contaminated water.…”

AmpC β-lactamases and bacterial resistance: an updated mini review

“…SDS-PAGE whole-cell protein profile analysis was used for the first time by Costas et al [105] for typing clinical isolates of Klebsiella aerogenes. Manchanda et al [106] used this technique as an effective adjunct to other methods of typing of isolates of AmpC-producing K. pneumoniae.…”

Comparative Protein Profile Analysis of Differentially Extracted Whole Cell Bacterial Protein Derived From Salmonella Typhi and Invasive Non-Typhoidal Salmonella

“…Several reports in literature showed the potential use of protein profiles for cost effective typing of bacteria [19]- [21]. Protein profiling by SDS-PAGE is an effective analytical technique used to produce high resolution separation of proteins based on their molecular weights [22].…”