Comparison of secondary plant metabolite production in cell suspension, callus culture and temporary immersion system

Wilken, Dirk; González, Elio Jiménez; Hohe, Annette; Jordán, Miguel; Kosky, Rafael Gómez; Hirschmann, Guillermo Schmeda; Gerth, André

doi:10.1007/1-4020-3200-5_39

Cited by 25 publications

(16 citation statements)

References 19 publications

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“…Samples produced under different culture conditions were taken for an analytical investigation of secondary metabolites production. As demonstrated before, the biomass productivity in the TIS system was three times higher than in microcontainers (Wilken et al, 2005).…”

Section: Tis Culturesupporting

confidence: 70%

Free Radical Scavengers from Cymbopogon citratus (DC.) Stapf Plants Cultivated in Bioreactors by the Temporary Immersion (TIS) Principle

Tapia

Cheel

Theoduloz

et al. 2007

Zeitschrift Für Naturforschung C

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The biomass production of Cymbopogon citratus shoots cultivated in bioreactors according to the temporary immersion (TIS) principle was assessed under different growth conditions. The effect of gassing with CO 2 -enriched air, reduced immersion frequency, vessel size and culture time on total phenolic and flavonoid content and free radical scavenging effect of the methanolic extracts was measured. From the TIS-culture of C. citratus, seven compounds were isolated and identified as caffeic acid (1), chlorogenic acid (2), neochlorogenic acid (3), p-hydroxybenzoic acid (4), p-hydroxybenzoic acid 3-O--d-glucoside (5), glutamic acid (6) and luteolin 6-C-fucopyranoside (7). The occurrence of compounds 1Ð7 and their variability in C. citratus grown under different TIS conditions was determined by HPLC. The free radical scavenging effect of the methanolic extract and compounds was measured by the discoloration of the free radical 1,1-diphenyl-2-picrylhydrazyl (DPPH). The main metabolites in 6-and 8-week-old cultures, both in 5 and 10 l vessels, were chlorogenic acid (2) (100Ð113 mg%) and neochlorogenic acid (3) (80Ð 119 mg%), while in the cultures with CO 2 -enriched air and reduced immersion frequency the main compound detected in the extracts was glutamic acid (6) (400 and 670 mg% for the green and white biomass and 619 and 630 mg% for the green and white biomass, respectively). The most active compounds, as free radical scavengers, in the DPPH discoloration assay were caffeic acid (1), chlorogenic acid (2), neochlorogenic acid (3) and the flavonoid luteolin 6-C-fucopyranoside (7).

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Section: Tis Culturesupporting

confidence: 70%

Free Radical Scavengers from Cymbopogon citratus (DC.) Stapf Plants Cultivated in Bioreactors by the Temporary Immersion (TIS) Principle

Tapia

Cheel

Theoduloz

et al. 2007

Zeitschrift Für Naturforschung C

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“…Bioreactors are usually designed for intensive culture and affording maximal opportunity for monitoring and control over microenvironmental conditions (Paek et al 2005). Several configurations of organ bioreactors are used for this purpose such as temporary immersion systems (Wilken et al 2005;Quiala et al 2006;Cheel et al 2007;Georgiev et al 2008). …”

Section: Introductionmentioning

confidence: 99%

“…Despite efforts made to produce plant secondary metabolites from cell cultures, only a few industrial applications have been successful. Since production of secondary metabolites is generally higher in differentiated tissue, there are attempts to use organ cultures for the production of medicinally important compounds (Wilken et al 2005).…”

Section: Introductionmentioning

confidence: 99%

Cardiotonic glycosides from biomass of Digitalis purpurea L. cultured in temporary immersion systems

Pérez-Alonso

Wilken²,

Gerth³

et al. 2009

Plant Cell Tiss Organ Cult

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Cardiotonic glycosides are extracted mostly from leaves of Digitalis plants. Commercial production of bioactive secondary metabolites by traditional agriculture is an inefficient process and can be affected by climatic and soil conditions. Strategies, based on in vitro culture methods, have been extensively studied to improve the production of specific plant derived chemicals. The aim of the present research was to obtain biomass of D. purpurea using the temporary immersion system (TIS) and to determine the content of cardiotonic glycosides (digitoxin, digoxin and lanatoside C) as secondary metabolites of commercial value for the pharmaceutical industry. Shoots were cultured in 1,000 ml TIS during 28 days. The effect of four immersion frequencies (once every 2, 4, 6, and 12 h) was studied. Biomass accumulation was influenced by immersion frequency. The maximum biomass accumulation (values in respect of fresh and dry weight) was obtained with immersions every 4 h (six immersions per day). HPLC analysis revealed the presence of digoxin and digitoxin for all immersion frequencies. No lanatoside C was detected in the biomass cultured in TIS. Digoxin concentrations varied depending on the frequencies tested. In contrast, the digitoxin content showed no dependency on the immersion frequency. Net production of digoxin and digitoxin per TIS were found to be higher with immersions every 4 h. The best net production of digitoxin and digoxin per TIS were 167.6 and 119.9 lg, respectively. The development of organ culture based on temporary immersion system can be a reliable method for the steady production of biomass for cardiotonic glycosides production, which is reported for the first time for Digitalis genus in this communication.

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“…Numerous secondary metabolites of medicinal uses were detected in cell suspension cultures of various plant species (Preil, 2005;Wilken et al, 2005). Several reports have been published on the establishment of cell suspensions of African oil palm (Elaeis guineensis) (de Touchet et al 1991;Teixeira et al, 1995;Aberlenc-Bertossi et al, 1999).…”

Section: Introductionmentioning

confidence: 99%

A comparative study between solid and liquid cultures relative to callus growth<br>and somatic embryo formation in date palm (Phoenix dactylifera L.) cv. Zaghlool

Ibraheem

Pinker

Böhme

2013

Emir. J. Food Agric

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Tissue culture techniques enable mass propagation of elite cultivars of date palm (Phoenix dactylifera L.). The main limitations for date palm in vitro multiplication are the low rates achieved using solidified medium and the long period needed to produce acclimatized plantlets. This research focuses on the comparison between different culture types and plant growth regulator (PGR) combinations on callus growth and somatic embryo formation of cv. Zaghlool. For callus growth, 200 mg friable embryogenic callus dispensed in Rasotherm and Phytacon flasks containing 200 ml liquid (100 rotations per minute) and in temporary immersion system, RITA ® bioreactor (5 min immersion every 12 h), were compared with cultures grown on 200 ml solidified medium. For somatic embryo formation, 500 mg of friable embryogenic callus grown in Erlenmeyer flasks filled with 50 ml liquid or solid MS medium. The medium was supplemented with 0.1 mg l -1 Naphthaleneacetic acid (NAA), 1.5 g l -1 activated charcoal (AC) with or without 0.05 mg l -1 6-Benzyl amino purine (BAP) compared to PGR-free medium. Results proved that cell suspension cultures produced the highest callus fresh mass as compared to the other systems tested, and the callus fresh mass reached 4 g after 16 weeks. The temporary immersion system did not significantly enhance the fresh mass of callus compared to the solidified medium. For somatic embryo induction, the number of somatic embryos increased in cell suspensions 6-16 fold compared to the solidified medium. Using liquid MS medium enriched with 0.1 mg l -1 NAA and 1.5 g l -1 AC gave rise to the highest number of somatic embryos formed from 500 mg initial callus: 160 embryos. The number of somatic embryos was also affected by the callus source. The calli induced from leaflet segments excised from converted somatic embryos resulted in a lower somatic embryo number than those of shoot tip origin with about 60 somatic embryos per 500 mg callus. The formation of somatic embryos using liquid medium required only 6 weeks, thus considerably reducing the previously reported period of 18 weeks which is required for somatic embryo formation using solidified media.

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Comparison of secondary plant metabolite production in cell suspension, callus culture and temporary immersion system

Cited by 25 publications

References 19 publications

Free Radical Scavengers from Cymbopogon citratus (DC.) Stapf Plants Cultivated in Bioreactors by the Temporary Immersion (TIS) Principle

Free Radical Scavengers from Cymbopogon citratus (DC.) Stapf Plants Cultivated in Bioreactors by the Temporary Immersion (TIS) Principle

Cardiotonic glycosides from biomass of Digitalis purpurea L. cultured in temporary immersion systems

A comparative study between solid and liquid cultures relative to callus growth<br>and somatic embryo formation in date palm (Phoenix dactylifera L.) cv. Zaghlool

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