Comparison of selected disinfectants efficiency against Listeria monocytogenes biofilm formed on various surfaces

Skowron, Krzysztof; Hulisz, Karolina; Gryń, Grzegorz; Olszewska, H.; Wiktorczyk, Natalia; Paluszak, Zbigniew

doi:10.1007/s10123-018-0002-5

Cited by 28 publications

(19 citation statements)

References 29 publications

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“…Different strains of L. monocytogenes also exhibited high MBCs for PAA in the range of up to 0.5% [28,29,30,31]. In contrast, others concluded that PAA was able to significantly reduce L. monocytogenes from multispecies biofilms at 0.15% [32] and 0.3% [30], respectively.…”

Section: Discussionmentioning

confidence: 99%

Characteristics of Listeria Monocytogenes Strains Persisting in a Meat Processing Facility over a 4-Year Period

et al. 2019

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Listeria monocytogenes can persist in food production facilities, resulting in serious threats to consumers due to the high mortality associated with listeriosis, especially in the very young, old and pregnant. We subtyped 124 strains of L. monocytogenes isolated from a meat processing facility in Switzerland by serotyping, multi locus sequence typing (MLST) typing and whole genome sequencing. We then analyzed their ability to form biofilms and their resistance to the disinfectants benzalkonium chloride (BC) and peracetic acid (PAA). The genotyping results of the strains showed that several clonal populations of L. monocytogenes belonging to CC9, CC204 and CC121 had persisted in this meat processing facility for at least four years. All of the strains showed biofilm forming capacity comparable to a known high biofilm forming strain. Known efflux pumps for BC were present in CC204, CC9 (brcABC) and CC121 (qacH) strains, while strains from other CC showed very low minimal inhibitory concentrations (MICs) for BC. For PAA, minimal bactericidal concentrations of 1.2–1.6% for 20 min and minimal inhibitory concentrations between 0.1 and 0.2% were observed. These values were close to or above the recommended concentration for use (0.5–1%), suggesting that PAA might be ineffective at controlling L. monocytogenes in this and potentially other meat processing facilities.

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Section: Discussionmentioning

confidence: 99%

Characteristics of Listeria Monocytogenes Strains Persisting in a Meat Processing Facility over a 4-Year Period

et al. 2019

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“…The identification of L. monocytogenes isolates was confirmed by PCR reaction. Two pairs of primers L1 (5′-CAG CAG CCG CGG TAA TAC-3′), L2 (5′-CTC CAT AAA GGT GAC CCT-3′), LM1 (5′-CCT AAG ACG CCA ATC GAA-3′) and LM2 (5′-AAG CAC TTG CAA CTG CTC-3′; Oligo.pl) were applied [ 22 ]. The PCR reaction mixture (25 μL) included 1× PCR buffer (Promega, Madison, WI, USA), 2 mM MgCl 2 (ABO, Gdańsk, Poland), 1.25 mmol dNTPs (Promega, Madison, WI, USA), 0.5 μM of each primer (Oligo.pl), 1 unit of Taq DNA polymerase (Promega, Madison, WI, USA), ultrapure water and DNA isolated from L. monocytogenes .…”

Section: Methodsmentioning

confidence: 99%

“…The identification of L. monocytogenes isolates was confirmed by PCR reaction. Two pairs of primers L1 (5 -CAG CAG CCG CGG TAA TAC-3 ), L2 (5 -CTC CAT AAA GGT GAC CCT-3 ), LM1 (5 -CCT AAG ACG CCA ATC GAA-3 ) and LM2 (5 -AAG CAC TTG CAA CTG CTC-3 ; Oligo.pl) were applied [22]. The PCR reaction mixture ( The PCR products were analyzed by agarose gel electrophoresis (1.5% agarose) stained with Midori Green (NIPPON Genetics EUROPE GmbH, Düren, Germany) in 1× TBE (Tris-boran-EDTA) buffer (BioRad, Hercules, CA, USA) using a DNA size standard (GeneRuler™ 1000 bp DNA Ladder; Fermentas, Waltham, MA, USA)-conditions 90 V, 1 h.…”

Section: Species Identificationmentioning

confidence: 99%

Assessment of the Prevalence and Drug Susceptibility of Listeria monocytogenes Strains Isolated from Various Types of Meat

Skowron

Wałecka-Zacharska

Wiktorczyk-Kapischke

et al. 2020

Foods

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Listeria monocytogenes are the etiological factor of listeriosis, and their main source for humans is food. The aim of the current study was to assess the contamination of various types of meat and the drug susceptibility of isolated L. monocytogenes. Between 2016–2018, 6000 swabs were taken (2000 annually) from the surface of pork, beef, and poultry. The analysis of intermediate and finished product samples was carried out in accordance with ISO 11290-1 (International Organization for Standardization). The genetic similarity assessment of the isolates obtained was based on the Pulsed Field Gel Electrophoresis (PFGE) method, and drug-sensitivity assessment using the disc-diffusion method. We found 2.1% of collected samples were L. monocytogenes positive. The level of meat contamination varied depending on its matrix. Most L. monocytogenes were isolated from poultry. It was shown that 39 (32.5%) strains were sensitive to all tested antibiotics and eight (6.7%) were resistant to all five tested antimicrobials. Most strains tested were resistant to cotrimoxazole (55; 45.8%) and meropenem (52; 43.3%), followed by erythromycin (48; 40.0%), penicillin (31; 25.8%), and ampicillin (21; 17.5%). High prevalence of this pathogen may be a serious problem, especially when linked with antibiotic resistance and high percentage of serotypes responsible for listeriosis outbreaks.

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“…Following the 24 h test period, a 100 μl aliquot of each well was transferred to 100 μl of Dey-Engley Neutralizing Broth (Sigma-Aldrich, St. Louis, MO, United States) and allowed to incubate for 2 minutes at room temperature. A 100 μl aliquot was then spread onto the surface of a MH agar plate and incubated at 37°C for 18–24 h (Skowron et al, 2018). The MBC was designated as the lowest biocide concentration which resulted in no viable growth of that isolate.…”

Section: Methodsmentioning

confidence: 99%

Comparative Genomics and Phenotypic Investigations Into Antibiotic, Heavy Metal, and Disinfectant Susceptibilities of Salmonella enterica Strains Isolated in Australia

et al. 2019

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Salmonella enterica is recognized as a major contributor of gastrointestinal illness worldwide. Concerns have been raised over the increasing prevalence of antibiotic resistant strains of Salmonella isolated from animals and food, and the role of antibiotics and other antimicrobial agents such as biocides and heavy metals in the selection and dissemination of antibiotic resistant bacteria to human hosts. In this study the antibiotic, heavy metal and disinfectant resistance genotypes and phenotypes of 19 S. enterica isolates from food-producing animals were established using whole genome sequence analysis, disc diffusion, as well as broth or agar dilution methods. This study also investigated the genomic environment of resistance genes on mobile genetic elements and chromosomal DNA. An ampicillin and streptomycin resistant S. Infantis isolate in this study harbored a β-lactamase ( bla TEM–1 ), and two streptomycin resistance conferring genes ( strA and strB ) on a class 1 integron mobilized on a large conjugative plasmid. This plasmid also harbored two arsenic resistance gene cassettes. The arsenic resistance cassette, arsRCDAB , was also observed in two S. Singapore isolates with high tolerance to arsenate. A nalidixic acid resistant S. Typhimurium isolate was found to possess a mutation in gyrA resulting in amino acid change Asp87Gly and tetracycline resistant S. Typhimurium isolate was found to harbor efflux pump gene, tetA . No resistance (genotypic or phenotypic) was recorded to the disinfectants screened in this study. Taken together, results of this study showed a good correlation between predicted and measured resistances when comparing genotypic and phenotypic data, respectively. The findings of this study do not suggest resistance to clinically relevant antibiotics are widespread among Salmonella isolated from Australian food-producing animals.

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Comparison of selected disinfectants efficiency against Listeria monocytogenes biofilm formed on various surfaces

Cited by 28 publications

References 29 publications

Characteristics of Listeria Monocytogenes Strains Persisting in a Meat Processing Facility over a 4-Year Period

Characteristics of Listeria Monocytogenes Strains Persisting in a Meat Processing Facility over a 4-Year Period

Assessment of the Prevalence and Drug Susceptibility of Listeria monocytogenes Strains Isolated from Various Types of Meat

Comparative Genomics and Phenotypic Investigations Into Antibiotic, Heavy Metal, and Disinfectant Susceptibilities of Salmonella enterica Strains Isolated in Australia

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