Comparison of Selected Non-Coding RNAs and Gene Expression Profiles between Common Osteosarcoma Cell Lines

Abstract

Osteosarcoma (OS) is a bone tumour affecting adolescents and elderly people. Unfortunately, basic treatment methods are still underdeveloped, which has a high impact on the poor survivability of the patients. Studies designed to understand the underlying mechanisms of osteosarcoma development, as well as preclinical investigations aimed at establishing novel therapeutic strategies, rely significantly upon in vitro models, which apply well-established cell lines such as U-2 OS, Saos-2 and MG-63. In this study, the expression of chosen markers associated with tumour progression, metastasis and survival were identified using RT-qPCR. Levels of several onco-miRs (miR-21-5p, miR-124-3p, miR-223-3p and miR-320a-3p) and long non-coding RNA MEG3 were established. The mRNA expression of bone morphogenetic proteins (BMPs), including BMP-2, BMP-3, BMP-4, BMP-6, BMP-7, as well as their receptors: BMPR-IA, BMPR-IB and BMPR-II was also determined. Other tested markers included metalloproteinases, i.e., MMP-7 and MMP-14 and survivin (BIRC5), C-MYC, as well as CYCLIN D (CCND1). The analysis included comparing obtained profiles with transcript levels established for the osteogenic HeLa cell line and human adipose-derived stromal cells (hASCs). The tested OS cell lines were characterised by a cancer-related phenotype, such as increased expression of mRNA for BMP-7, as well as MMP-7 and MMP-14. Osteosarcoma cells differ considerably in miR-21-5p and miR-124-3p levels, which can be related to uncontrolled tumour growth. The comprehensive examination of osteosarcoma transcriptome profiles may facilitate the selection of appropriate cell models for preclinical investigations aimed at the development of new strategies for OS treatment.

Whole-transcriptome sequencing and bioinformatics analysis of calcified outer-layer tissue from cystic echinococcosis

“…Several studies have shown miR-223 to have regulatory potential (Taïbi et al, 2014;Jung et al, 2022;Sikora et al, 2022;Tanigawa et al, 2022). Furthermore, miR-223-3p was found to be highly expressed in active-phase E. granulosus tissue (Mariconti et al, 2019).…”

Targeting nerve growth factor-mediated osteosarcoma metastasis: mechanistic insights and therapeutic opportunities using larotrectinib

“…Extensive research has implicated MMPs in cancer progression [2]. MMP-2, MMP-7, MMP-9, and MMP-14 have been linked to the progression and metastasis of osteosarcoma [40,41]. Xu et al reported that berberine inhibits the migration and invasion of osteosarcoma cells by downregulating the expression of MMP-2 and MMP-9s [42].…”

Signal Pathways and microRNAs in Osteosarcoma Growth and the Dual Role of Mesenchymal Stem Cells in Oncogenesis

“…Many studies have shown that miRNAs are abnormally regulated in human malignancies, including OS, and act as a novel class of oncogenes or tumor suppressors. Literature data on the role of miRNAs in various OS cell lines suggest an important role of miRNAs in the progression of OS [26,27]. Other studies suggest that miRNAs play a central role in the pathogenesis of OS [24].…”

“…In addition to the cell subpopulations described, MSCs from bone marrow and adipose tissue (hASCs) [27,343], which are involved in cell proliferation, progression, and metastasis, are an important component of the OS tumor microenvironment [344].…”