Comparison of serological techniques for the diagnosis of equine infectious Anemia in an endemic area of Argentina

Espasandin, A. G.; Cipolini, M. F.; Forletti, Agustina; Díaz, Santiago; Soto, Javier; Martínez, David; Storani, C. A.; Monzón, N. M.; Beltrame, Juan I.; Lucchesi, Esteban; Soto, Pedro

doi:10.1016/j.jviromet.2021.114101

Cited by 7 publications

(3 citation statements)

References 15 publications

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“…However, its key disadvantages are the long turnaround time for the diagnostic results, high antibody concentrations and difficult visual reading of the precipitation line (Cullinane et al, 2007;Issel and Cook 1993;Scicluna et al, 2013). Serological diagnosis by ELISA is the most rapid and potentially sensitive technique for EIA, detecting infections earlier than the AGID test (Espasandin et al, 2021;Nardini et al, 2017).…”

Section: Resultsmentioning

confidence: 99%

Prevalence of equine infectious anemia virus in horses and donkeys determined by comparison of ELISA and AGID in Mexico

Villa-Mancera,

Villegas-Bello,

Campos-García

et al. 2024

Arq. Bras. Med. Vet. Zootec.

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From February to December 2021, 960 serum samples from different climate regions in two Mexican states were analyzed for antibodies against EIAV using competitive enzyme-linked immunosorbent assay (cELISA) and agar gel immunodiffusion (AGID) tests. The overall seroprevalence of EIAV infection in equids determined by ELISA and AGID tests was 19.69% and 17.60%, respectively. The highest rate of seropositivity was observed in the state of Veracruz (23.67%, using AGID test), and the lowest rate was found in the state of Puebla (13.12%, using cELISA test). In total, 17.45% (85/487, using AGID test) and 15.40% (75/487, using cELISA test) of horses were positive for EIAV infection. Donkeys showed a prevalence of 19.87% (94/473) in cELISA and 21.99% (104/476) in AGID. The cELISA was compared with AGID as the gold standard test. The diagnostic sensitivity for horses and donkeys was 90.4% and 92.2%, and the specificity was 97.5% and 97.3%, respectively, with an almost perfect degree of agreement (kappa values of 0.871 and 0.888). Therefore, the overall seroprevalence rate of EIAV infection in equines in the sampled states of Puebla and Veracruz was 19.3%, with 91.4% sensitivity, 97.4% specificity, and an agreement of 96.25%, indicating almost perfect agreement (kappa = 0.880).

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Section: Resultsmentioning

confidence: 99%

Prevalence of equine infectious anemia virus in horses and donkeys determined by comparison of ELISA and AGID in Mexico

Villa-Mancera,

Villegas-Bello,

Campos-García

et al. 2024

Arq. Bras. Med. Vet. Zootec.

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“…Interestingly, no clinical signs were observed which magnifies their epidemiological role. The disease is highly endemic in South American countries have one of the largest equine populations (48). EIAV tends to reoccur after a long gap of time as in India1999 then 2012 (32).…”

Section: Prevalence and Geographical Distributionmentioning

confidence: 99%

Epidemiological aspects of some equine viral diseases

Ata¹,

Shaapan²,

Ghazy³

et al. 2023

IJVS

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Although different equine viruses' outbreaks have been recorded. However, the most important ones in are the African horse sickness virus (AHSV), equine influenza virus (EIV), equine viral arteritis (EVA), Equine infectious anaemia virus (EIAV), and equine herpes viruses (EHV). To combat these diseases, it is imperative to understand their epidemiological aspects. So, the current review aims to highlight some epidemiological aspects including; causative agents, clinical forms, history, prevalence and geographical distribution, source of infection, and methods of transmission. The AHSV mainly causes pulmonary, and cardiac forms with high morbidity and mortality rates in Africa. The EIV is found all over the world and results in respiratory signs. The EVA has low morbidity and mortality rates and is mainly found in the Americas and Europe, its significance is due to the reproductive problems as abortion in mares and subfertility in stallions. The EIAV has low morbidity and mortality rates and causes long time course disease mainly of fever, and chronic anaemia or death. The EHVs are the current most important pathogens due to their endemicity all over the world and their high morbidity. It causes respiratory, abortion, neonatal, and sometimes neurological manifestations. Aerosols and body excretions are the main sources of infection with EIV, EVA, and EHV. Venereal EVA transmission occurs through natural breeding or artificial insemination with the semen of infected or carrier stallions. The spreading of arboviruses is greatly affected by the vector activity like the AHSV which transmitted by the Culicoide imicola biting midges, and the EIAV by family Tabanidae. In general, it is recommended to take all epidemiological measures, including vaccinations and vector control, to limit the spread of such diseases and reduce economic losses.

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“…Arthropods such as mosquitoes, flies, mites, fleas, lice and ticks can act as vectors of viruses and other pathogens via active biological transmission but also through passive mechanical transmission [9,[13][14][15][16] (Table 1, Ref. [17][18][19][20][21][22][23][24][25][26][27][28][29][30]). Biological transmission occurs when vectors transmit pathogens that multiply within their bodies [31,32].…”

Section: Possible Role Of Arthropod Vectors At the Host-sars-cov-2 In...mentioning

confidence: 99%

Challenges at the host-arthropod-coronavirus interface and COVID-19: a One Health approach

Fuente

Mera

Gortázar

2021

Front. Biosci. (Landmark Ed)

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Comparison of serological techniques for the diagnosis of equine infectious Anemia in an endemic area of Argentina

Cited by 7 publications

References 15 publications

Prevalence of equine infectious anemia virus in horses and donkeys determined by comparison of ELISA and AGID in Mexico

Prevalence of equine infectious anemia virus in horses and donkeys determined by comparison of ELISA and AGID in Mexico

Epidemiological aspects of some equine viral diseases

Challenges at the host-arthropod-coronavirus interface and COVID-19: a One Health approach

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