Comparison of serological tests forBrucella melitensis infection in sheep

Mahajan, N. K.; Kulshreshtha, R. C.

doi:10.1007/bf02361263

Cited by 13 publications

(15 citation statements)

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“…In addition, SAT performed slightly better (147 positive versus 139) than RBPT for the detection of Brucella antibodies, which is in agreement with previous reports (Mahajan and Kulshreshtha 1991). These variations may be due to the ability of each test to detect different antibody classes; The RBPT which is widely used as a screening test for brucellosis performs better to detect IgG and the SAT can detect immunglobulins of both IgG and IgM classes.…”

Section: Discussionsupporting

confidence: 91%

“…The tests employed here were able to detect brucellosis in a range of 33.7 to 36.7%, which supports the view that a single test is often inadequate to effectively detect brucellosis (Bercovich et al 1998;Mahajan and Kulshreshtha 1991). Therefore, at least two serological tests should be combined in order to detect brucellosis most effectively in an infected sheep flock.…”

Section: Discussionsupporting

confidence: 79%

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Seroepidemiological investigation of brucellosis in sheep abortions in Kars, Turkey

Celebi

Atabay

2008

Trop Anim Health Prod

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This study was undertaken to investigate the seroprevalence of brucellosis in unvaccinated sheep from the flocks having previous abortion cases in Kars and around, Turkey and to compare the efficacy of each serological test used. Four hundred serum samples collected from 16 different flocks of sheep having a history of abortions in Kars and its surrounding area in Turkey were examined for the presence of antibodies raised against Brucella using Rose Bengal Plate Test (RBPT), Serum Agglutination Test (SAT), Rivanol Agglutination Test (RAT) and Complement Fixation Test (CFT). All animals were unvaccinated against Brucella. Of the serum samples tested, 147 (%36.7), 142 (%35.5), 139 (%34.75) and 135 (%33.75) were found positive by SAT, RAT, RBPT and CFT, respectively. No statistically significant difference was found between the serological tests used (p> 0.05). It is concluded from this study that brucellosis continues to be an important problem for ovine abortions and poses a risk both for human and other animals in this area. Therefore, adequate intervention measures should be implemented to control and eradicate brucellosis. In addition, if conventional serological tests are used at least two tests, RPBT for screening and CFT for the confirmation of the positive samples are preferable, should be used in parallel for detection of brucellosis effectively.

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Section: Discussionsupporting

confidence: 91%

Section: Discussionsupporting

confidence: 79%

Seroepidemiological investigation of brucellosis in sheep abortions in Kars, Turkey

Celebi

Atabay

2008

Trop Anim Health Prod

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“…Although isolation and identification of organism are considered gold standard for diagnosis, it is cumbersome, takes several days to weeks, and poses a greater risk to laboratory personnel. Hence, the diagnosis of brucellosis largely depends on the use of two or more tests to confirm any positive animals [ 20 , 21 ]. RBPT is a screening test, and iELISA is a confirmatory test used for B. melitensis infection in sheep and goats [ 19 , 21 , 22 ] .…”

Section: Discussionmentioning

confidence: 99%

Seroprevalence of brucellosis in small ruminants in organized and unorganized sectors of Gujarat state, India

Kanani¹,

Dabhi²,

Patel³

et al. 2018

Vet World

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Aim:The present study aimed to study the seroprevalence of brucellosis in small ruminants of Gujarat state, India, using Rose Bengal Plate test (RBPT) and indirect enzyme-linked immunosorbent assay (iELISA).Materials and Methods:A total of 2444 sera samples (675 sheep and 1769 goat) from unorganized sector and 1310 sera samples (861 sheep and 449 goat) from seven organized farms were collected for brucellosis screening.Results:In unorganized sector, 23.70% sheep (160/675) and 15.99% goat (283/1769) were positive by RBPT and 24.44% sheep (165/675) and 17.24% goat (305/1769) by iELISA. The organized sector samples showed higher seroprevalence in goat (7.79 %, 35/449) than sheep (4.06 %, 35/861) by RBPT. Similarly, in iELISA, goat samples showed a higher seroprevalence (9.35%, 42/449) compared to sheep (7.50%, 65/861). The diagnostic sensitivity and specificity of RBPT with ELISA were 88.69% and 99.65%, respectively, and showed a significant difference (p≤0.0001). The Chi-square analysis revealed a significant difference in seroprevalence between sectors (p≤0.01) and species (p≤0.01).Conclusion:The seroprevalence of brucellosis in small ruminants of Gujarat was investigated and showed a higher prevalence of brucellosis and warrants the implementation of proper preventive measures.

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“…Another technique that has been widely used in certain countries is the standard agglutination test (SAT). This assay shows, however, a lower sensitivity and specificity than others, such as RBT and FCT in small ruminants [46,[95][96][97]. Still, its usefulness in these animal species has been demonstrated [98].…”

Section: Other Indirect Diagnostic Tests Based On the Humoral Immune mentioning

confidence: 99%

Control of Animal Brucellosis — The Most Effective Tool to Prevent Human Brucellosis

García‐Seco¹,

Domı́nguez²,

Álvarez³

2015

Updates on Brucellosis

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The World Health Organization classifies brucellosis as one of the seven neglected endemic zoonosis which contribute to the perpetuation of poverty in developing countries. Although most of the developed countries are free from this important zoonosis, brucellosis has still a widespread distribution in the Mediterranean region, the Middle East, Central Asia, and parts of Latin America, making it a global problem. Nearly half a million of new cases of human brucellosis are reported each year around the world, in which animals (or products of animal origin) are the most likely source of infection. Brucella melitensis, the main etiologic agent of small ruminant brucellosis, is the most prevalent specie involved in cases of human disease in most parts of the world. Additionally, Brucella abortus (main responsible of bovine brucellosis) and Brucella suis (the most common etiological agent of porcine brucellosis) are often associated with human brucellosis. In animal production, brucellosis has a strong economic impact due not only to its direct consequences (e.g., reproductive failures) but also to indirect loses (e.g., trade restrictions). The problem of brucellosis could be considered a clear example of the need for a "One World, One Health" strategy, given that the only approach to achieve its control and subsequent eradication is the cooperation between public and animal health authorities. The prevention of human brucellosis cannot be achieved without the control of the disease in the animals, as exemplified by the impact that the early measures adopted in the beginning of the 20th century forbidding the consumption of goat milk had on the prevalence of the disease in the British soldiers in Malta. When the prevalence of the disease in the animal population is high or when eradication cannot be achieved due to other factors (e.g., lack of economic resources), its control in livestock must be the first objective. When deciding the optimal approach to tackle the disease, the prevalence of animal brucellosis is not the only parameter to consider by the decision makers since other epidemiological and economic aspects should be considered in order to implement the most adequate control strategy in each region. Cooperation between all stakeholders involved is a cornerstone in the success of any control strategy. Strict biosafety and management measures, vaccination, and test-and-slaughter strategy are recognized as the most ef

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Comparison of serological tests forBrucella melitensis infection in sheep

Cited by 13 publications

References 7 publications

Seroepidemiological investigation of brucellosis in sheep abortions in Kars, Turkey

Seroepidemiological investigation of brucellosis in sheep abortions in Kars, Turkey

Seroprevalence of brucellosis in small ruminants in organized and unorganized sectors of Gujarat state, India

Control of Animal Brucellosis — The Most Effective Tool to Prevent Human Brucellosis

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