Comparison of Serum Tumor Associated Material (TAM) with Conventional Biomarkers in Cancer Patients

Shu, Jiwu; Li, Cheng-Guang; Liu, Yangchen; Yan, Xiao-Chun; Xu, Xu; Huang, Xin-En; Cao, Jie; Li, Ying; Lu, Yen‐Shen; Wu, Xueyan; Jin, Ling; Xiang, Jin

doi:10.7314/apjcp.2012.13.5.2399

Cited by 48 publications

(15 citation statements)

References 31 publications

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“…The above hypothesis test was a two-sided test; P<0.05 was considered as statistically significant. We have enough experience in conducting medical researches, and have published some results elsewhere Yan et al, 2011;Zhang et al, 2011;Gong et al, 2012;Liu et al, 2012;Gu et al, 2013;Shu et al, 2012;Xu et al, 2012;Xu et al, 2012;Yu et al, 2012;Zhang et al, 2012;Chen et al, 2013;Dai et al, 2013;Deng et al, 2013;Huang et al, 2013;Lu et al, 2013;Sun et al, 2013;Wei et al, 2013;Wu et al, 2013;Yang et al, 2013;.…”

Section: Statistical Methods and Research Experiencementioning

confidence: 99%

CXCL12-CXCR4 Promotes Proliferation and Invasion of Pancreatic Cancer Cells

Shen

Zheng²,

Lu³

et al. 2013

Asian Pacific Journal of Cancer Prevention

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Objective: CXCL12 exerts a wide variety of chemotactic effects on cells. Evidence indicates that CXCL12, in conjunction with its receptor, CXCR4, promotes invasion and metastasis of tumor cells. Our objective was to explore whether the CXCL12-CXCR4 biological axis might influence biological behavior of pancreatic cancer cells. Methods: Miapaca-2 human pancreatic cancer cells were cultured under three different conditions: normal medium (control), medium + recombinant CXCL12 (CXCL12 group), or medium + CXCR4-inhibitor AMD3100 (AMD3100 group). RT-PCR was applied to detect mRNA expression levels of CXCL12, CXCR4, matrix metalloproteinase 2 (MMP-2), MMP-9, and human urokinase plasminogen activator (uPA). Additionally, cell proliferation and invasion were performed using CCK-8 colorimetry and transwell invasion assays, respectively. Results: CXCL12 was not expressed in Miapaca-2 cells, but CXCR4 was detected, indicating that these cells are capable of receiving signals from CXCL12. Expression of extracellular matrix-degrading enzymes MMP-2, MMP-9, and uPA was upregulated in cells exposed to exogenous CXCL12 (P<0.05). Additionally, both proliferation and invasion of pancreatic cancer cells were enhanced in the presence of exogenous CXCL12, but AMD3100 intervention effectively inhibited these processes (P<0.05). Conclusions: The CXCL12-CXCR4 biological axis plays an important role in promoting proliferation and invasion of pancreatic cancer cells.

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Section: Statistical Methods and Research Experiencementioning

confidence: 99%

CXCL12-CXCR4 Promotes Proliferation and Invasion of Pancreatic Cancer Cells

Shen

Zheng²,

Lu³

et al. 2013

Asian Pacific Journal of Cancer Prevention

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“…Each point on the ROC curve represents a determined threshold. Statistical analyses were performed using IBM SPSS statistics 19.0 for Windows (SPSS Inc; IBM, Chicago, IL).We have published medical researches that were conducted in Jiangsu Cancer Center elsewhere Yan et al., 2011; Zhang et al, 2011; Gong et al, 2012;Liu et al, 2012; Gu et al, 2013;Li et al, 2012;Shu et al, 2012; Zhan et al, 2012; Zhan et al, 2012;Xu et al, 2012;Xu et al, 2012;Yu et al, 2012; Zhang, et al, 2012; Zhang et al, 2012; Chen et al, 2013; Dai et al, 2013; Deng et al., 2013; Liu et al, 2013; Liu et al, 2013; Liu et al, 2013; Lu et al, 2013;Sun et al, 2013;Wei et al, 2013;Wu et al, 2013;Yang et al, 2013; …”

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confidence: 99%

Acidic Pelvic Drainage as a Predictive Factor For Anastomotic Leakage after Surgery for Patients with Rectal Cancer

Liu

Huang²,

Xu³

et al. 2013

Asian Pacific Journal of Cancer Prevention

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considerable extra cost, increased in-hospital morbidity and mortality, impaired pelvic organ function (Eriksen et al., 2005; Law et al., 2007; Lee et al., 2008;Riss et al., 2011). Although there are multiple risk factors, such as being male, advanced age, severe obesity, lower tumor site (less than 4cm from the anal verge) and the reduction of TSGF in POD5, which are associated with anastomotic leakage have been reported in our previous study (Yang in individual patients on account of the absence of a early stage.The diagnosis of AL should be as early as possible for the sake of reducing its associated morbidity and mortality (Doeksen et al., 2007). Many studies on anterior resections regarding AL come from multi-center and different surgeons. A variety of factors predisposing to AL have been reported in the previous investigations. However, lack of data about the risk factors and incidence associated with AL from a single-institute of one team of doctors. Liu Yang et al 5442In addition, a great deal previous studies are repetitive works and duplicated effforts because of the limited power of innovative thinking. The pathophysiological processes and considering that interstitial acidic pH notion that extracellular acidosis may intensify the acute and extending its functional lifespan (Trevani et al., 1999; Martínez et al., 2006). The acidic microenvironments developing and the pelvic draining is becoming acidic, which is the main theoretical foundation for the present research. This study aimed to evaluate the utility of sequential postoperative pelvic drainage determinations of of AL after anterior resection of rectal cancer, which has not been reported ever before. Materials and MethodsBetween January 2004 and December 2011, 753 consecutive patients with rectal cancer underwent anterior resection with double stapling anastomosis for primary Jiangsu Cancer Hospital of Nanjing Medical University & Jiangsu Institute of Cancer Research, Nanjing, China. The medical notes of all patients were reviewed in detail.of TNM stage, histologically proven adenocarcinoma, open and laparoscopic surgery with pelvic drainage, all patients with 12 postoperative determinations of pH value, and antibiotics using for 7 PODs. Exclusion criteria were of TNM stage, colon cancer, hand-sewn anastomosis and perioperatively abdominopelvic implants that might be affect pH value, ongoing infection before surgery or an acquired infection in the postoperative period other the distance from the anal verge, as determined by rigid sigmoidoscopy. Total mesorectal excision was adopted as the standard surgical technique according to tumor location. The Ethics Committee of Science approved data collection in the register.All the patients were assigned to one of two groups according to the presence or absence of AL: with AL (Group AL, n=57), without AL (Group nAL, n=696). The two groups were compared according to the following characteristics: median age, gender, physical status (ASA score), the alcohol and tobacco abuse, tumor site, TNM stage, pre...

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“…These results suggested that macrophage-like THP-1 cells facilitate angiogenesis in tumors to provide rapidly proliferating transformed-cells much more oxygen and nutrients. The proangiogenic role of TAMs has been characterized by previous studies that showed the correlation between TAMs infiltration and high number of vascular in many tumors, such as squamous cell carcinoma of the esophagus, prostate cancer and ameloblastoma (Koide et al, 2004;Halin et al, 2009;Guzman-Medrano et al, 2012), the main reason for this may be that TAMs produce many cytokines for angiogenesis: vascular endothelial growth factor (VEGF), platelet-derived growth factor (PDGF), TNF-α, transforming growth factor-β (TGF-β) (Bingle et al, 2006;Araujo et al, 2010;Shu et al, 2012), and several angiogenesis-modulating enzymes, such as matrix metalloproteinases (MMP) (Pollard, 2004;Kale et al, 2014). The fact that the specific proangiogenic program in tumor turns on indicated that tumor is in a condition of hypoxic, rendering tumor cells to angiogenesis.…”

Section: Discussionmentioning

confidence: 99%