Comparison of seven hepatitis <scp>B</scp> virus (<scp>HBV</scp>) nucleic acid testing assays in selected samples with discrepant <scp>HBV</scp> marker results from <scp>U</scp>nited <scp>S</scp>tates blood donors

Enjalbert, Florence; Krysztof, David E.; Candotti, Daniel; Allain, Jean‐Pierre; Stramer, Susan L.

doi:10.1111/trf.12653

Cited by 29 publications

(39 citation statements)

References 21 publications

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“…Therefore implementing a highly sensitive assay for HBV DNA screening in Lebanese blood donors, should be considered to avoid any false negative results. Previous studies have shown that various NAT commercial assays differ in their sensitivity (36)(37)(38), this could be associated with various factors such Hepat Mon. 2017; 17 (7):e14250.…”

Section: Discussionmentioning

confidence: 99%

Low Prevalence of Occult Hepatitis B Infection Among Blood Donors in Beirut, Lebanon: Reconsider the Deferral Strategy of Anti-HBc Positive Blood Donors

et al. 2017

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Background: Hepatitis B virus (HBV) infection remains one of the major infectious threats to human health. Since the implementation of highly sensitive HBV nucleic acid testing, occult HBV infection (OBI) has been detected. Occult HBV infection is characterized by a positive HBV DNA test with undetectable HBsAg (Hepatits B surface antigen). The prevalence of OBI varies significantly between geographic areas, genotypes, and population depending on the sensitivity of the detection assays used.

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Section: Discussionmentioning

confidence: 99%

Low Prevalence of Occult Hepatitis B Infection Among Blood Donors in Beirut, Lebanon: Reconsider the Deferral Strategy of Anti-HBc Positive Blood Donors

et al. 2017

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“…However, HBV transfusion-transmission cannot be completely excluded since the pooling procedure reduces the assay's analytical sensitivity [9], and in fact a recent report indicated that the likelihood of detecting OBI donations by NAT increased when the size of the pool was decreased from 50 to 20 donations or when the test sensitivity was increased by a small margin [9][10][11]. As recently pointed out [5], studies on the safety of the current screening policies would require the testing of matched recipient specimens collected before and after transfusion.…”

Section: Introductionmentioning

confidence: 97%

Poor efficacy of nucleic acid testing in identifying occult HBV infection and consequences for safety of blood supply in Italy

Spreafico¹,

Berzuini²,

Foglieni³

et al. 2015

Journal of Hepatology

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“…Many countries have chosen to reduce the number of samples/pool by using mini pool (MP) testing, thus increasing the analytical sensitivity [66]. The detection of OBI donations by NAT is improved when the MP is reduced from 50 to 20 samples [33].…”

Section: Detection Of Occult Hepatitis B Infections In Blood Donorsmentioning

confidence: 99%

Occult Hepatitis Infection in Transfusion Medicine: Screening Policy and Assessment of Current Use of Anti-HBc Testing

Esposito

Sabia

Iannone

et al. 2017

Transfus Med Hemother

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HBV still represents a global risk factor in transfusion medicine. The residual risk of HBV is not limited to pre-seroconversion window period but it extends to donors with occult HBV infection (OBI) characterized by the presence of HBV DNA in liver and by the absence of the virus surface antigen. Each country developed an appropriate blood screening policy according to local HBV prevalence, yields of infectious units per different screening methods and cost-effectiveness. We underline the need of maintaining a high level of attention for OBI carrier identification in all blood banks worldwide where the screening procedures are generally based on a combination of both serological markers and nucleic acid amplification test. In this context, markers such as hepatitis B surface antibodies and hepatitis B core antibodies (anti-HBc) might be useful, although the use of this latter is highly debated and still controversial. Our aim is to give an overview on the relevant diagnostic approaches for the routine screening for HBV focusing on the feasibility of anti-HBc testing as precautionary measure in preventing OBI transmission worldwide. In our tailored algorithm, the loss of about 1% of ‘anti-HBc only' donors, does not significantly affect the blood supply while improving recipient safety.

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Comparison of seven hepatitis B virus (HBV) nucleic acid testing assays in selected samples with discrepant HBV marker results from United States blood donors

Abstract: Highly sensitive NATs require new confirmatory algorithms as presented optimally using different genomic regions or sequence generation. The introduction of immigration-related HBV genotypes may impact HBV epidemiology in the United States.

Cited by 29 publications

References 21 publications

Low Prevalence of Occult Hepatitis B Infection Among Blood Donors in Beirut, Lebanon: Reconsider the Deferral Strategy of Anti-HBc Positive Blood Donors

Low Prevalence of Occult Hepatitis B Infection Among Blood Donors in Beirut, Lebanon: Reconsider the Deferral Strategy of Anti-HBc Positive Blood Donors

Poor efficacy of nucleic acid testing in identifying occult HBV infection and consequences for safety of blood supply in Italy

Occult Hepatitis Infection in Transfusion Medicine: Screening Policy and Assessment of Current Use of Anti-HBc Testing

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