Comparison of signature lipid methods to determine microbial community structure in compost

Steger, Kristin; Jarvis, Åsa; Smårs, Sven; Sundh, Ingvar

doi:10.1016/s0167-7012(03)00187-8

Cited by 80 publications

(63 citation statements)

References 33 publications

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“…Fatty acid extraction by the EL FAME or MIDI method was done after 0, 56, and 125 days of incubation on 1 g of compost placed in a screw-cap test tube. The procedures used derived from that of a previous similar study (29) with slight modifications necessary for compost (32). EL procedure.…”

Section: Methodsmentioning

confidence: 99%

“…Investigations of structural and functional changes in soil microbial communities have been done using several methods, including the fatty acid profiling method (14,27,29). This procedure has also been used as an early and sensitive method to investigate microorganism succession during the composting process (32), together with the composition of long-chain fatty acids (26,28).…”

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confidence: 99%

“…Recent studies show a short-term effect on fatty acids during composting, with the main decrease being between days 3 and 24 of composting (32), and also a fatty acid profile shift in months-long experiments (2).…”

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confidence: 99%

“…Methods to determine the fatty acid composition of the microorganisms have thus been developed, such as the microbial identification (MIDI) system and the ester-linked (EL) procedure (25,29). The application of these methods to compost samples is recent, and little is known about the extent to which they can give comparable information (32).…”

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confidence: 99%

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Effect of Pesticide Inoculation, Duration of Composting, and Degradation Time on the Content of Compost Fatty Acids, Quantified Using Two Methods

Cardinali

Otto

Vischetti

et al. 2010

Appl Environ Microbiol

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Compost biobeds can promote biodegradation of pesticides. The microbial community structure changes during the composting process, and simple methods can potentially be used to follow these changes. In this study the microbial identification (MIDI) and ester-linked (EL) procedures were used to determine the composition of fatty acid methyl esters (FAMEs) in composts aged 3 and 12 months, inoculated with 3 recalcitrant pesticides (azoxystrobin, chlorotoluron, and epoxyconazole and a coapplication of all three) after 0, 56, and 125 days of degradation. Pesticide persistence was high, and after 125 days the residue was 22 to 70% of the applied amount depending mostly on the composting age. Seventy-one FAMEs belonging to nine groups were detected. The EL method provided three times as many detections as did the MIDI method and was more sensitive for all FAME groups except alcohol. Thirty-six and five FAMEs were unique to the EL and MIDI methods, respectively. The extraction method was of importance. The EL method provided a higher number of detections for 57 FAMEs, and the MIDI method provided a higher number for 9 FAMEs, while the two methods were equal for 5 FAMEs; thus, the EL method provided a more uniform overall FAME profile. Effects of the other factors were not always clear. Inoculation with pesticide did not influence the FAME profile with the MIDI method, while it influenced cyclopropane and monounsaturated content with the EL method. Composting age and degradation time had an effect on some groups of FAMEs, and this effect was greater with the EL method. The use of some FAMEs as biomarkers to follow microbial community succession was likely influenced by the type of compost and other factors.Plant protection has become a key factor in intensive agriculture, but the widespread use of pesticides can be toxic to nontarget organisms and can lead to ecosystem alterations. Formulated chlorotoluron (a herbicide), azoxystrobin (a fungicide), and epoxyconazole (a fungicide) are commonly used at the postemergence stage mainly for cereals, to control broadleaved weeds and grasses and to control some foliar and soilborne diseases, respectively.Most information on pesticide persistence is derived from registration documents, few studies appear in the scientific literature, and independent research is always suggested. A recent pesticide database (Footprint, 2007; http://www.eu -footprint.org/ppdb.html, accessed April 2010) reports half-life values (days) in soil, derived from laboratory studies at 20°C, of 73.5 for azoxystrobin, 59 for chlorotoluron, and 226 for epoxyconazole. However, a certain variability exists, and in other research half-lives are longer, i.e., more than 2 years (at 10°C) for epoxyconazole (4), 62 to 107 days for azoxystrobin (10), and 30 to 200 days for chlorotoluron (36), showing that significant degradation should be measured on a time scale of months or years (8, 9) according to the results of a recent review on pesticide persistence (3).The use of such persistent pesticides on agricultural fi...

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Section: Methodsmentioning

confidence: 99%

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confidence: 99%

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confidence: 99%

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confidence: 99%

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Effect of Pesticide Inoculation, Duration of Composting, and Degradation Time on the Content of Compost Fatty Acids, Quantified Using Two Methods

Cardinali

Otto

Vischetti

et al. 2010

Appl Environ Microbiol

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“…In addition, dimethyl disulfide de rivatization for identification and quantification of monounsaturated fatty acids followed previous procedures (Steger et al 2003). Standard nomenclature was used to refer to the PLFAs, and based on the concentrations of 43 individual PLFAs, the total microbial biomass was calculated (Steger et al 2011).…”

Section: Fatty Acid Analysismentioning

confidence: 99%

Comparative study on bacterial carbon sources in lake sediments: the role of methanotrophy

Steger¹,

Premke²,

Gudasz³

et al. 2015

Aquat. Microb. Ecol.

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Methane-derived carbon can be important in both benthic and pelagic food webs. Either generated in the anaerobic layers of the sediment or in the anaerobic hypolimnion of stratified eutrophic lakes, methane is an excellent carbon source for aerobic methanotrophic bacteria. The very negative methane δ 13 C-signal in the methanotrophic biomass provides an excellent opportunity to trace the use of methane-derived carbon in food webs. We studied carbon sources of benthic bacteria in a range of Swedish lakes with different inputs of terrestrial organic carbon and indigenous primary production. We analyzed the 13 C: 12 C ratios in phospholipid-derived fatty acids, which serve as biomarkers for specific groups of Bacteria. We demonstrate that methane is an important carbon source for sediment bacteria, not only for the methanotrophic community but also for the non-methanotrophic heterotrophic bacteria. This most likely indirect utilization of isotopically highly depleted methane masks the stable isotope signatures for terrestrial input and local primary production in the heterotrophic bacterial community.

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2010

Wastewater Microbiology

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Comparison of signature lipid methods to determine microbial community structure in compost

Cited by 80 publications

References 33 publications

Effect of Pesticide Inoculation, Duration of Composting, and Degradation Time on the Content of Compost Fatty Acids, Quantified Using Two Methods

Effect of Pesticide Inoculation, Duration of Composting, and Degradation Time on the Content of Compost Fatty Acids, Quantified Using Two Methods

Comparative study on bacterial carbon sources in lake sediments: the role of methanotrophy

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