Comparison of silver-staining nucleolar organizer region (AgNOR) counts and proliferating cell nuclear antigen (PCNA) expression in reactive mesothelial hyperplasia and malignant mesothelioma

Bethwaite, Peter; Delahunt, Brett; Holloway, Linda; Thornton, Ann

doi:10.1080/00313029500169362

Cited by 15 publications

(11 citation statements)

References 16 publications

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“…Congruously, MM cells show a high proliferation rate as evidenced by a high mitotic count and by methods to demonstrate proliferating cell nuclear antigens and silver-stained nucleolar organizing regions. 22,23 An autocrine stimulatory effect of growth factors such as insulin-like growth factor-1 (IGF-1) and plateletderived growth factors (PDGFs) via their receptors has been postulated in the pathogenesis of malignant mesothelioma. 24,25 Interestingly, the stimulatory role in angiogenesis of both of these growth factors has been observed in vitro.…”

Section: Discussionmentioning

confidence: 99%

Evaluation of Tumour Angiogenesis as a Prognostic Marker in Malignant Mesothelioma

et al. 1997

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Section: Discussionmentioning

confidence: 99%

Evaluation of Tumour Angiogenesis as a Prognostic Marker in Malignant Mesothelioma

et al. 1997

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“…4,22 The results obtained by various research groups consistently show that a rising AgNOR count is indicative of an increasing tendency toward proliferation; however, there are differences between the values obtained. 2,5,10,16,27,33,34 Using histologic or cytologic samples, the AgNOR technique is suitable for distinguishing lymphoma cases according to prognosis (poor or better) as reported by several authors. 16,17,26,29 According to some authors, however, it is the increase of the total area of AgNORs within the nucleus, rather than the number of AgNORs, that shows a close correlation with the rate of proliferation and the prognosis.…”

Section: Introductionmentioning

confidence: 99%

“…It has been demonstrated that the number of AgNORs per nucleus can be regarded as one of the hallmarks of proliferation and that mean AgNOR counts are good indicators of the degree of malignancy of human non-Hodgkin's lymphoma. 2,10,11,20,24,28,33,34 Moreover, AgNOR counts were also found to be indicators of postsurgical prognosis in canine mast cell tumors. 4,22 The results obtained by various research groups consistently show that a rising AgNOR count is indicative of an increasing tendency toward proliferation; however, there are differences between the values obtained.…”

Section: Introductionmentioning

confidence: 99%

Use of the Argyrophilic Nucleolar Region Method for Cytologic and Histologic Examination of the Lymph Nodes in Dogs

et al. 2004

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Abstract. Argyrophilic nucleolar organizer region (AgNOR) counts in cytologic and histologic lymph node samples from healthy dogs and dogs with lymphoma were compared. Control samples were taken from 10 Beagle dogs (six female and four male dogs, 1.5-2 years), and lymphoma samples were taken from 16 dogs. Cytologic samples were obtained by fine-needle aspiration and impression and histologic samples by excision or incisional biopsy. Altogether, 26 cytologic, 19 excisional, and 7 incisional biopsy samples were examined. Lymph nodes of controls showed a moderate inflammatory pattern; of the lymphoma cases, nine were lowgrade forms and seven were high-grade forms. Mean AgNOR counts per nucleus were determined. AgNOR counts were statistically different (P Ͻ 0.001) between controls and lymphoma cases in cytologic (1.35 and 3.59, respectively) and histologic (1.4 and 2.89, respectively) samples. In lymphoma cases, AgNOR counts in cytologic samples were higher than those in histologic samples by 0.81 (P Ͻ 0.001) and showed a linear relationship (r ϭ 0.6; P Ͻ 0.05) with the histologic counterparts in excisional biopsy samples. AgNOR counts in low-and high-grade lymphomas were significantly different (P Ͻ 0.05) in cytologic (3.21 and 4.08, respectively) and histologic (2.68 and 3.18, respectively) samples. In conclusion, AgNOR counts were higher in lymph nodes with lymphoma than in reactive nodes. In the case of dogs with lymphoma, AgNOR counts in cytologic samples were linearly related to excisional but not to incisional biopsy samples. Although AgNOR counts were different between cytologic and histologic samples, either sample type provided enough sensitivity to differentiate between high-and low-grade forms of lymphoma.

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“…[49][50][51][52][53][54][55][56][57][58] In addition, the counting of silver-stained nucleolar organizer regions (AgNORs) (loops of DNA encoding ribosomal RNA that associate with argyrophilic nonhistone proteins, which can be visualized with silverstaining) has been suggested as a method for the differential diagnosis of MM from benign reactive hyperplasia. 48,59,60 On average, MMs have a higher proliferation rate and a higher AgNOR count than reactive mesothelial hyperplasia, but because of a significant overlap in results, these markers have no value in the discrimination between benign and malignant mesothelial proliferations in pathology practice.…”

Section: Markers Of Benign Versus Malignantmentioning

confidence: 99%

Epithelioid Lesions of the Serosa

Anttila

2012

Archives of Pathology &Amp; Laboratory Medicine

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Context.—Diagnosing epithelioid serosal lesions remains a challenge because numerous different processes—primary or secondary, benign or malignant—occur in body cavities, some of which are very rare. Objectives.—To review the newest literature and to describe the morphologic criteria and immunohistochemical markers that are useful for distinguishing epithelioid serosal lesions. Data Sources.—Previously published literature concentrating on the newest research findings. Earlier reviews are principally referred to for established diagnostic criteria. Conclusions.—Immunohistochemistry with a panel of antibodies has made the diagnosis of epithelioid serosal lesions very reliable. When deciding on antibodies used in differential diagnosis, it is important to consider tumor location, clinical and radiologic information, and morphologic features. Immunohistochemistry is less useful in the differential diagnosis of benign versus malignant mesothelial lesions. The diagnosis of benign versus malignant mesothelial proliferations still relies on the histologic criteria of invasion.

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Comparison of silver-staining nucleolar organizer region (AgNOR) counts and proliferating cell nuclear antigen (PCNA) expression in reactive mesothelial hyperplasia and malignant mesothelioma

Cited by 15 publications

References 16 publications

Evaluation of Tumour Angiogenesis as a Prognostic Marker in Malignant Mesothelioma

Evaluation of Tumour Angiogenesis as a Prognostic Marker in Malignant Mesothelioma

Use of the Argyrophilic Nucleolar Region Method for Cytologic and Histologic Examination of the Lymph Nodes in Dogs

Epithelioid Lesions of the Serosa

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