Comparison of simultaneous measurement of mouse locomotor activity by radiotelemetry and photobeam methods

Streng, Tomi; Klemola, Tero; Bastman, Sanna; Nevalainen, Jaakko; Scheinin, Mika

doi:10.1016/j.vascn.2014.09.003

Cited by 2 publications

(2 citation statements)

References 17 publications

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“…The manufacturer DSI uses changes of signal strength as the mouse moves across the receiver plate to derive an “activity count”, which reflects amount and speed of motion per time. This is a semi-quantitative measure, which gives results comparable to the established beam break method 48 , and has been used to compare the overall activity between groups of mice 49 . To obtain a continuous rather than a discrete measure (as in DSI’s “activity count”), we expressed activity as the standard deviation of the signal strength values for each epoch, which gives a redundant (but continuous) measure.…”

Section: Methodsmentioning

confidence: 99%

The natural Disc1-deletion present in several inbred mouse strains does not affect sleep

Dittrich

Petese

Jackson

2017

Sci Rep

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The gene Disrupted in Schizophrenia-1 (DISC1) is linked to a range of psychiatric disorders. Two recent transgenic studies suggest DISC1 is also involved in homeostatic sleep regulation. Several strains of inbred mice commonly used for genome manipulation experiments, including several Swiss and likely all 129 substrains, carry a natural deletion mutation of Disc1. This constitutes a potential confound for studying sleep in genetically modified mice. Since disturbed sleep can also influence psychiatric and neurodegenerative disease models, this putative confound might affect a wide range of studies in several fields. Therefore, we asked to what extent the natural Disc1 deletion affects sleep. To this end, we first compared sleep and electroencephalogram (EEG) phenotypes of 129S4 mice carrying the Disc1 deletion and C57BL/6N mice carrying the full-length version. We then bred Disc1 from C57BL/6N into the 129S4 background, resulting in S4-Disc1 mice. The differences between 129S4 and C57BL/6N were not detected in the 129S4 to S4-Disc1 comparison. We conclude that the mutation has no effect on the measured sleep and EEG characteristics. Thus, it is unlikely the widespread Disc1 deletion has led to spurious results in previous sleep studies or that it alters sleep in mouse models of psychiatric or neurodegenerative diseases.

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Section: Methodsmentioning

confidence: 99%

The natural Disc1-deletion present in several inbred mouse strains does not affect sleep

Dittrich

Petese

Jackson

2017

Sci Rep

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“…When the animal cut off the light beam falling on the photocell, a count was picked. These cut-offs were counted for a period of 10 min 19 . All mice of either sex were divided into five groups of six animals each.…”

Section: Spontaneous Locomotor Activity (Slma)mentioning

confidence: 99%

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2022

IJPSR

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A chronic illness that affects a person's mood, thoughts, physical health, and behavior patterns is considered depression. Currently used anti-depressant drugs such as selective serotonin reuptake inhibitors (SSRI) and tricyclic anti-depressants (TCA) are showing various side effects and thus, the search for a new anti-depressant herb without side effects is important. The intend of the present study was to evaluate the anti-depressant effect of methanol, chloroform and aqueous extract Acacia nilotica leaves at doses of 200 mg/kg in experimental animals. In-vivo screening models like Forced Swim Test (FST), Tail Suspension Test (TST) and Spontaneous Locomotor activity (SLMA) were used to assess the anti-depressant effects of the methanol, chloroform, and aqueous extracts of Acacia nilotica Linn. The dried-up leaves were subjected for shade drying and size reduced to powder and extracted by soxhlet apparatus. The extracts were subjected to phytochemical tests and the carbohydrate, tannins, alkaloids, flavonoids, saponins, glycosides and steroids were found to be present. Imipramine (30 mg/kg, i.p) was used as the reference standard, and it showed significant anti-depressant activity in mice. The significant anti-depressant effects of Acacia nilotica could be due to the strong and effective concentration of the active constituent. In the FST model, MEAN (200 mg/kg,po) showed high significant decreases in immobility time (137.3 ± 1.67**). In TST model, MEAN (200 mg/kg, po) showed highly significant decreases in immobility time (147.50 ± 1.19***). In SLMA showed less significant increases in locomotor activity scores (228.2 ± 4.37*). From the literature surveys as well experiments performed, it can be understood that Acacia nilotica does pose anti-depressant property.INTRODUCTION: Depression is a chronic, recurrent psychiatric disorder affecting nearly 21% of the world's population 1, 2 . It is characterized by emotional and physical manifestations, such as feelings of worthlessness, helplessness, guilt or QUICK RESPONSE CODE

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Comparison of simultaneous measurement of mouse locomotor activity by radiotelemetry and photobeam methods

Cited by 2 publications

References 17 publications

The natural Disc1-deletion present in several inbred mouse strains does not affect sleep

The natural Disc1-deletion present in several inbred mouse strains does not affect sleep

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