Comparison of single and joint effects of Zn and Cu in continuous flow and batch reactors

Şengör, S. Sevinç; Gikas, Petros; Moberly, James G.; Peyton, Brent M.; Ginn, Timothy R.

doi:10.1002/jctb.2730

Cited by 19 publications

(9 citation statements)

References 51 publications

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“…The observed biomass and glucose concentrations with time for the four reactor treatments are given in Figs , for each of the reactors 1–4, respectively. As discussed by Sengor et al, the metal‐free control reactor (# 1) showed nearly constant OD values, around 0.13, while glucose concentrations were below detection throughout the experimental period (Fig. ).…”

Section: Resultssupporting

confidence: 60%

“…Four similar parallel completely mixed flow reactors, operating under identical conditions (apart from the concentration of toxic metal) were used to investigate the impact of single and joint exposure of Zn and Cu on Arthrobacter sp. Our previous study demonstrated the significance of continuous flow conditions on the microbial growth and toxicity of Zn and Cu. As a continuation of that work, the current study presented here explored the impact of individual and combined exposure of these toxic metals on the energy status (in terms of ATP pool) and tolerance of surviving microorganisms growing in continuous systems.…”

Section: Resultsmentioning

confidence: 94%

“…The reactors were operating with a flow rate of 0.5 mL min −1 , resulting in a residence time of 11 h, based on a specific growth rate of Arthrobacter sp. JM018 observed during batch cultivation . Metals were introduced into the system as injections of calculated volumes of Zn and/or Cu solutions into both the inlet medium carboy tanks (feeding the reactors) and into the individual reactors, so that the desired concentrations were rapidly achieved in each treatment reactor.…”

Section: Methodsmentioning

confidence: 99%

“…Microbial growth was monitored by measuring the OD of each sample withdrawn at regular intervals from the reactors using a Genesys™ 10 Series Spectrophotometer (Thermo Electron Corporation) at 600 nm. Optical density (600 nm) was linearly correlated with biomass concentration . Glucose concentrations were measured using the Glucose (HK) Assay Kit (SIGMA; Saint Louis, Missouri, USA).…”

Section: Methodsmentioning

confidence: 99%

“…Although previous studies have examined the resistance and inhibition of metals in continuous and batch systems, the activity and recovery of viable cells under continuous exposure to single and combined toxic metals have not yet been investigated. Our previous study demonstrated the significance of continuous flow conditions to evaluate the individual and combined effects of Zn and Cu (i.e. 125 µmol L ‐1 Zn; 40 µmol L ‐1 Cu; and mixture of 125 µmol L ‐1 Zn plus 40 µmol L ‐1 Cu) on the growth of microbial cultures.…”

Section: Introductionmentioning

confidence: 99%

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Single and joint effects of Zn and Cu to ATP pool and microbial recovery in continuous growth systems

Şengör

Gikas

Moberly

2018

J of Chemical Tech & Biotech

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BACKGROUND: Four parallel continuous stirred tank reactors were used to investigate the single and joint exposure of Zn and Cu to Arthrobacter sp. JM018, in terms of the impact on the adenosine triphosphate (ATP) pool and microbial tolerance. RESULTS: ATP, optical density (OD), and substrate concentration measurements indicated that Cu was more toxic than Zn under all conditions studied. The results showed that although both OD and ATP measurements individually reflected a decrease in microbial growth rate after the addition of metals for the reactors exposed to Cu, specific ATP (i.e. ATP/OD) showed only a temporary reduction followed by a monotonic return to pre-exposure levels within 100 h. The latter implied tolerance and recovery in the energy status of these cells, after the initial shock due to the exposure to Cu. CONCLUSION: Specific ATP is an important quantity to be considered as a measure of the activity or energy status of microbial biomass surviving after exposure to toxic metals or other unfavorable conditions. The study provides insights for maintaining metal-tolerant microbial communities and to explore both quantitatively and experimentally the dynamics of ATP pool and microbial tolerance in metal-contaminated environments.

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Section: Resultssupporting

confidence: 60%

Section: Resultsmentioning

confidence: 94%

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Single and joint effects of Zn and Cu to ATP pool and microbial recovery in continuous growth systems

Şengör

Gikas

Moberly

2018

J of Chemical Tech & Biotech

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Investigation of copper inhibition of nitrifying moving bed biofilm (MBBR) reactors during long term operations

Schopf

Delatolla

Mathew

et al. 2018

Bioprocess Biosyst Eng

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Copper, a prevalent heavy metal in industrial mining wastewaters, has been shown to inhibit nitrification in wastewater treatment systems. Biofilm treatment systems have an inherent potential to reduce inhibition. This study investigated the effects of copper concentration on nitrifying biofilms in moving bed biofilm reactor (MBBR) systems across long term operation using influent ammonia concentrations representative of gold mining wastewater. Conventional isotherm models did not adequately model the attachment of copper to the biofilm. Long term nitritation was shown to be uninhibited at influent copper concentrations between 0.13 and 0.61 mg Cu/L. Nitratation was inhibited with influent copper concentrations of 0.28-0.61 mg Cu/L. There was no statistical difference in biofilm characteristics, including biofilm thickness, mass and density, across all copper concentrations tested, however, changes in biofilm morphology were observed. The demonstrated resistance of the nitrifying biofilm to copper inhibition makes the MBBR system a promising technology for treating ammonia in mining wastewaters.

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Fluidized-bed denitrification for mine waters. Part II: effects of Ni and Co

et al. 2013

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The dispersion of nitrogenous compounds and heavy metals into the environment is frequent during mining activities. The effects of nickel (Ni) and cobalt (Co) on denitrification of simulated mine waters were investigated in batch bioassays and fluidized-bed reactors (FBRs). At pH 7, batch tests revealed that Co did not exhibit inhibition on denitrification even at 86.6 mg/L. Ni showed to be inhibitory at 50 and 100 mg/L by decreasing nitrate removal efficiencies of 18 and 65 %, respectively. In two FBRs, operated at 7-8 and 22 °C, 5.5 mg/L Ni did not affect nitrate and nitrite removals because of FBR potential of diluting soluble Ni feed concentration. On the contrary, the effluent pH clearly decreased in both FBR1 and FBR2 because of nickel sulfide precipitation and Ni inhibition of the last two steps of denitrification. When Ni injection was stopped, the process recovered more slowly at 22 than 7-8 °C. This is the first study reporting the effect of Ni on denitrification in biological FBRs.

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Comparison of single and joint effects of Zn and Cu in continuous flow and batch reactors

Cited by 19 publications

References 51 publications

Single and joint effects of Zn and Cu to ATP pool and microbial recovery in continuous growth systems

Single and joint effects of Zn and Cu to ATP pool and microbial recovery in continuous growth systems

Investigation of copper inhibition of nitrifying moving bed biofilm (MBBR) reactors during long term operations

Fluidized-bed denitrification for mine waters. Part II: effects of Ni and Co

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