Comparison of single- and multi-strain probiotics effects on broiler breeder performance, egg production, egg quality and hatchability

Abstract

1. This study was conducted to investigate the effect of multi-strain probiotic (containing Lactobacillus acidophilus 2.5 × 10 cfu/g, Lactobacillus casei 2.5 × 10 cfu/g, Bifidobacterium thermophilum 2.5 × 10 cfu/g and Enterococcus faecium 2.5 × 10 cfu/g) and single-strain probiotic (Pediococcus acidilactici 1 × 10 cfu/g) on broiler breeder performance and gastrointestinal health. 2. A completely randomised trial was conducted using 300 broiler breeder hens (Ross 308) aged 51 weeks old which were randomly allocated to 1 of 5 dietary treatments with 6 replicates per treatment in a 10 week trial. Treatments included (1) the basal diet a negative control, (2) basal diet supplemented with 0.1 g/kg multi-strain probiotic (MS), (3) basal diet supplemented with 0.1 g/kg single-strain probiotic (SS), (4) basal diet supplemented with 0.1 g/kg of both of probiotics (MS+ SS) and (5) positive control basal diet supplemented with 0.5 g/kg oxytetracycline antibiotic (OX). 3. Body weight, egg production, yolk weight, eggshell thickness and weight, Haugh unit, fertility and hatchability were determined. Results showed that dietary treatments had no significant effect on total hen house or total hatching egg production, egg weight, yolk colour index, shell weight, mortality, body weight, fertility, hatchability, oviduct and stroma weight or number of large and small yellow follicles (P > 0.05). None of the jejunum morphological parameters, apparent ileal digestibility of protein and ileal Lactobacillus population were influenced by supplemental probiotics (P > 0.05), although ileum Escherichia coli count was reduced by inclusion of dietary probiotics (P < 0.05). 4. It was concluded that although both probiotic treatments reduced coliforms, they did not improve broiler breeder performance or gastrointestinal tract (GIT) function.

Effects of Bacillus subtilis PB6 supplementation on productive performance, egg quality and hatchability in broiler breeder hens under commercial farm condition

“…In the present study, total mortality did not differ among the dietary groups during seven weeks of the experiment. Similar to our observations, previous studies revealed that the mortality of the laying hens (Khan et al 2011) and broiler breeders (Aalaei et al 2018) was not influenced by the supplementation of probiotics. This may be due to the fact that continuous feeding of probiotic might have suppressed the undesirable microorganisms that lead to improved health status (Khan et al 2011).…”

“…Use of Lactobacillus sporogenes in White Leghorn (WL) layer breeders (Panda et al 2008) and Pediococcus acidilactici in Hy-Line Brown laying hens (Mikulski et al 2012) as probiotic improved eggshell thickness. Use of single-and multi-strain probiotics had no significant effect on eggshell thickness in broiler breeders (Aalaei et al 2018).…”

“…At the end of experiment, ten faecal samples were collected under aseptic conditions from the selected birds in each house by pressing the outer wall of cloaca to push its content into sterile tubes. To determine Lactobacillus and Escherichia coli counts, 1-g samples were taken and serially diluted from 10 −1 to 10 −7 with sterile ice-cold anoxic phosphate buffer saline (Aalaei et al 2018). Lactobacillus count was performed in Rogosa agar after incubation in an anaerobic chamber at 37°C for 48 h. The E. coli count was performed in MacConkey agar after aerobic incubation at 37°C for 24 h. The numbers of colony-forming units (CFUs) were expressed as log10 CFU per gram (Tuohy et al 2002).…”

“…Beneficial effects of probiotics on broiler performance (Apata 2008), nutrient digestibility (Li et al 2008), modulation of intestinal microflora (Yu et al 2008), pathogen inhibition (Mountzouris et al 2009), and immunomodulation and gut mucosal immunity (Teo and Tan 2007) have been reported. There are many studies on the effects of probiotics on the performance of broiler chickens, but few numbers of researches evaluated the beneficial effect of probiotics on broiler breeders (Bozkurt et al 2011;Hajati et al 2014;Zaghari et al 2017;Aalaei et al 2018).…”

Supplementation of Lactic Acid Bacteria Derived from Ensiled Kumpai Tembaga on Live Body Weight, Gastrointestinal Tract, Internal Organs, and Blood Profiles in Pegagan Ducks

“…A reverse result was also reported by Reis et al (2017) that birds supplemented with B. subtilis definitely presented a reduced relative duodenum length. On the other hand, Aalaei et al (2018) also reported that none of the jejunal morphological parameters changes in broilers supplemented with probiotics. It can be considered that variations in the strains, sources, viability, and concentrations of bacteria, and methods of administration might be the main factors causing different responses in poultry gastrointestinal tract.…”