Comparison of Six Commercial ELISA Kits for Their Specificity and Sensitivity in Detecting Different Major Peanut Allergens

Jayasena, Shyamali; Smits, Mieke; Fiechter, Daniëlle; Jong, Aard de; Nordlee, Julie A.; Baumert, Joe L.; Taylor, Steve L.; Pieters, Raymond; Koppelman, Stef J.

doi:10.1021/jf504741t

Cited by 71 publications

(55 citation statements)

References 40 publications

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“…A similar observation has been reported by Jayasena et al with these two kits with the NIST (National Institute of Standards and Technology, Gaithersburg, MD) standard reference material 2387 peanut butter and is most likely due to the calibration of the kits for which the kit manufacturers supply their own independent standard in absence of international standard materials (Jayasena et al, 2015). The ranges of reactivity of the samples are from 2.8 to 4.5 mg/mL (mean ± SD ¼ 4.0 ± 0.5) for the Neogen kit, and 0.44 to 0.92 (mean ± SD ¼ 0.6 ± 0.1) mg/ml for the Morinaga kit (Table 3).…”

Section: Reactivity In Commercial Kits For Peanut Residue Detection Asupporting

confidence: 79%

“…The ranges of reactivity of the samples are from 2.8 to 4.5 mg/mL (mean ± SD ¼ 4.0 ± 0.5) for the Neogen kit, and 0.44 to 0.92 (mean ± SD ¼ 0.6 ± 0.1) mg/ml for the Morinaga kit (Table 3). Jayasena et al (2015) showed that these two kits have distinctly different specificities for the major allergens. The Neogen kit detects predominantly Ara h 3 with about a 30-fold lower reactivity to Ara h 1, while Ara h 2 and Ara h 6 are minimally recognized.…”

Section: Reactivity In Commercial Kits For Peanut Residue Detection Amentioning

confidence: 99%

“…The Neogen kit detects predominantly Ara h 3 with about a 30-fold lower reactivity to Ara h 1, while Ara h 2 and Ara h 6 are minimally recognized. The Morinaga kit recognizes Ara h 2 and Ara h 6, but had very low recognition of Ara h 1 and Ara h 3 (both about 1000-fold less reactive; Jayasena et al, 2015). A comparison was made between the peanut protein content of the samples assayed with the Morinaga kit and the sum of Ara h 2 and Ara h 6 as determined by RP-HPLC.…”

Section: Reactivity In Commercial Kits For Peanut Residue Detection Amentioning

confidence: 99%

“…The validation of the effectiveness of cleaning protocols can be monitored using immunoassays to detecting peanut residue on equipment surfaces and quantifying peanut residue in food samples. Such immunoassays have different sensitivities for specific peanut allergens (Jayasena et al, 2015), i.e. some detect mainly Ara h3, and others detect mainly Ara h2 and Ara h2.…”

Section: Introductionmentioning

confidence: 99%

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Allergenicity attributes of different peanut market types

Koppelman

Jayasena

Luykx

et al. 2016

Food and Chemical Toxicology

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a b s t r a c tFour different market classes of peanut (Runner, Virginia Spanish, and Valencia) are commonly consumed in Western countries, but for some consumers peanuts are a main cause of food-induced anaphylaxis. Limited information is available on the comparative allergenicity of these distinct market classes. The aim of this study was to compare allergenicity attributes of different peanut cultivars.The protein content and protein profiles were highly comparable for all tested cultivars. All cultivar samples contained the major allergens Ara h 1, Ara h 2, Ara h 3 and Ara h 6, as assessed by SDS-PAGE and RP-HPLC, although some minor differences in major allergen content were found between samples. All samples were reactive in commercial ELISAs for detection and quantification of peanut protein. IgEbinding potency differed between samples with a maximum factor of 2, indicating a highly comparable allergenicity.Based on our observations, we conclude that peanuts from the main market types consumed in Western countries are highly comparable in their allergenicity attributes, indicating that safety considerations with regard to peanut allergy are not dependent on the peanut cultivar in question.

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Section: Reactivity In Commercial Kits For Peanut Residue Detection Asupporting

confidence: 79%

Section: Reactivity In Commercial Kits For Peanut Residue Detection Amentioning

confidence: 99%

Section: Reactivity In Commercial Kits For Peanut Residue Detection Amentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Allergenicity attributes of different peanut market types

Koppelman

Jayasena

Luykx

et al. 2016

Food and Chemical Toxicology

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“…When comparing ELISA assays available for the detection of peanut proteins it becomes evident that they differ in their ability to recover peanut proteins in terms of their specificity, LOD, and quantification range, as summarized by Jayasena et al [30]. Ara h 2 and Ara h 6 have been shown to be hardly recognized by these assays in contrast to Ara h 3, which was the most recognized allergen.…”

Section: Discussionmentioning

confidence: 99%

Detection of the Peanut Allergens Ara h 2 and Ara h 6 in Human Breast Milk: Development of 2 Sensitive and Specific Sandwich ELISA Assays

Schocker

Scharf

Kull

et al. 2017

Int Arch Allergy Immunol

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Background: Little is known about breast milk as a vehicle for tolerance development or sensitization to peanuts very early in life. Thus, well-characterized and highly sensitive detection systems for the reliable determination of peanut allergens in breast milk are mandatory. Methods: For the quantification of the marker allergens Ara h 2 and Ara h 6 in the low nanogram per milliliter range in breast milk samples of a German cohort, sensitive and highly specific sandwich ELISAs were optimized and validated. Results: The Ara h 2 ELISA revealed a limit of detection (LOD) of 1.3 ng Ara h 2/mL and a quantification range of 2.3-250 ng/mL, the Ara h 6 ELISA showed an LOD of 0.7 ng/mL and a working range of 1.1-14.4 ng/mL. The assays showed no relevant cross-reactivity against other potentially cross-reactive legume, seed, and tree nut extracts (<0.01%, except for Ara h 1 in the Ara h 2 ELISA <0.1%). Ara h 2 was detectable in breast milk samples from 14/40 (35%) of the participants in concentrations from 2.3 to 184 ng/mL, Ara h 6 appeared in 9/40 (22.5%) of the lactating mothers between 1.1 and 9.7 ng/mL, and 1 highly positive sample with 79 ng/mL. Both allergens appeared at the same time points, but Ara h 6 in lower concentrations than Ara h 2. Conclusions: Sensitive and specific diagnostic tools for the determination of Ara h 2 and Ara h 6 in human breast milk were established. The kinetics of secreted Ara h 2 and Ara h 6 seem to be similar but with a difference in concentration. Follow-up investigations on their tolerogenic or sensitizing properties in breast milk become now accessible.

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Recent Development on the Determination of Allergens in Food

Nitride,

Ferranti,

D'Auria

et al. 2024

Encyclopedia of Analytical Chemistry

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Risk assessment of unintended food allergens, contaminants in the food production chain, relies on the availability of analytical methods capable of providing accurate measurements. DNA‐ and immune‐based assays are first action methods providing within a short time frame information about the measurand, expressed as milligrams of total protein of the allergenic ingredient per kilogram of food product. On the other hand, isotope dilution mass spectrometry ( IDMS ) serves as a confirmatory method by providing peptide absolute quantification with a small measurement uncertainty, achieved by removing instrumental fluctuations. This analytical approach is a step forward to metrological traceable methods for food allergen analysis. All the detection methods can measure DNA, single peptides, or proteins, while the allergenic consumer protection and the action levels to support the quantitative risk assessments are based on the total mass of the allergenic food proteins. This article provides an overview of the analytical methods to be used in the frame of food allergen detection with a focus on methodological advancement.

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Comparison of Six Commercial ELISA Kits for Their Specificity and Sensitivity in Detecting Different Major Peanut Allergens

Cited by 71 publications

References 40 publications

Allergenicity attributes of different peanut market types

Allergenicity attributes of different peanut market types

Detection of the Peanut Allergens Ara h 2 and Ara h 6 in Human Breast Milk: Development of 2 Sensitive and Specific Sandwich ELISA Assays

Recent Development on the Determination of Allergens in Food

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