Comparison of slide coagglutination test and countercurrent immunoelectrophoresis for detection of group B streptococcal antigen in cerebrospinal fluid from infants with meningitis

Webb, Bette J.; Edwards, Morven S.; Baker, Carol J.

doi:10.1128/jcm.11.3.263-265.1980

Cited by 21 publications

(10 citation statements)

References 8 publications

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“…Compared with coagglutination, counterimmunoelectrophoresis is more cumbersome and time-consuming to perform. Additionally, in a study by Webb et al (12), coagglutination proved more rapid and, in some instances, more sensitive than counterimmunoelectrophoresis for the detection of streptococcal antigens in cerebrospinal fluid.…”

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confidence: 98%

“…Blood cultures and other body fluids are some of the most important specimen types processed in the laboratory, since results can significantly influence the therapy and final outcome of the patient. Therefore, many investigators have focused their attention on improving reporting time with these specimens (1,5,8,11,12). Direct testing of fluids by counterimmunoelectrophoresis and coagglutination has proven to be a valuable asset in the early detection of organisms (1,5,8,12).…”

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confidence: 99%

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Direct Testing of Blood Cultures for Detection of Streptococcal Antigens

1982

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A direct, rapid, and simple method for the detection of streptococcal antigens of Lancefield groups A, B, C, D, and G from blood cultures was developed by using a coagglutination test. Fifty-five clinical specimens and 117 simulated blood cultures containing gram-positive cocci were tested. Out of 6,261 clinical blood cultures screened, 55 cultures from 53 patients were positive, with organisms resembling streptococci, by Gram stain. Of these cultures, 78% (43 of 55) were pure cultures of streptococci, and 22% (12 of 55) were mixed with at least one other organism. Of the 43 pure cultures only, correct reactions were obtained (grouping correctly or giving no cross-reactions, or both) with 86% (37 of 43) of the isolates, 12% (5 of 43) exhibited cross-reactions, and 2% (1 of 43) gave false-negative reactions. All of the cross-reacting isolates were Streptococcus pneumoniae , which reacted with the group C reagent, and the false-negative reaction occurred with a Streptococcus bovis isolate. However, by using a direct modified bile solubility test, the correct identification of the S. pneumoniae isolates was obtained. Therefore, by using the modified bile solubility test in conjunction with the direct grouping method, 98% (42 of 43) of the isolates in pure culture could be identified accurately and rapidly after the detection of a positive Gram stain. Correct grouping reactions were obtained with 83% (10 of 12) of the mixed blood cultures, and false-negative results occurred with 17% (2 of 12) of them. Both cultures contained an enterococcus and a gram-negative rod. Of the 117 simulated blood cultures, there was only one incorrect grouping reaction; this occurred with an S. bovis isolate that cross-reacted with the group C reagent. The direct grouping reaction was positive when blood cultures contained a minimum of 1 × 10 8 to 8 × 10 8 colony-forming units per ml. In general, this procedure provided information on the identification of the organism 24 h earlier than by conventional identification methods.

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confidence: 98%

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Direct Testing of Blood Cultures for Detection of Streptococcal Antigens

1982

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“…In this study, the development of an immunological assay for REV was evaluated utilizing staphylococcal protein-A coagglutination. Antibodycoated staphylococci have been shown to agglutinate when mixed with the homologous antigen (2,4,9,17,19). This agglutination assay is very simple and can be performed within two hours, requiring only a specific mammalian antiserum directed against the antigen, a preparation of antibody treated staphylococci, and the antigen.…”

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confidence: 99%

“…been used in the development of agglutination assays for the identification of streptococci (2) and Neisseria gonorrhoeae (4). This procedure has also been used for the detection of Hemophilus influenzue type b and other antigens in body fluids (17,19).…”

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Staphylococcal Protein‐a Agglutination Assay for Avian Viruses

1981

Acta Pathologica Microbiologica Scandinavica Section C Immunolo

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Protein‐A rich staphylococci were used to develop an agglutination assay for the detection and quantitation of avian reticuloendotheliosis virus (REV) and Herpes virus of turkeys (HVT). Rabbit anti‐REV or anti‐HVT were used to coat the staphylococci and these reagents were mixed with REV or various other viral antigen preparations. An agglutination titer was determined for antigen preparations of varying purity and the results indicated that in addition to being rapid, this assay allows the detection of as little as 0.3 to 0.5 μg of partially purified viral antigens. No crossreactivity was observed with the two antisera and the various antigen preparations. This study also confirmed the reports in the literature that chicken immunoglobulins do not bind to protein‐A of the Cowan I strain of Staph, aureus.

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“…Because group B Streptococcus (GBS) is a frequent cause of life-threatening bacteremia and meningitis in neonates and young infants (1), rapid diagnostic methods for detection of the group B-specific polysaccharide antigen in body fluids have been developed. Among these methods, latex agglutination (LA) and slide coagglutination have had the best sensitivity (88 to 100%) (2,3,5,6,8,11,12), but a somewhat lower specificity (94 to 98%) (2,6,8,11) than countercurrent immunoelectrophoresis (CIE) (100%) (2,4,7,10). The hyperimmune rabbit antisera employed in these commercially available reagents are polyclonal and lot to lot variable with respect to GBS antibody titer.…”

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Detection of group B streptococcal antigen in body fluids by a latex-coupled monoclonal antibody assay

Rench¹,

Metzger²,

Baker³

1984

J Clin Microbiol

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A commercially available latex agglutination reagent, Directigen (Hynson, Westcott & Dunning, Div. Becton Dickinson & Co., Baltimore, Md.), in which a murine monoclonal antibody to group B streptococcal (GBS) antigen is the active component, was evaluated by using body fluid specimens from 94 sick infants. Antigen was detected in one or more admission specimens from 18 of 19 (94.7%) infants with symptomatic GBS infection. In 15 patients with GBS meningitis, cerebrospinal fluid, serum, and 10-fold-concentrated urine specimens were positive in 87, 50, and 100%, respectively. GBS antigen was detected in 50% of unconcentrated urines, but in no sera from infants with nonmeningitic GBS sepsis. Among specimens from 27 infants with invasive infection due to organisms other than GBS and from 48 culture-negative sick infants, false-positive latex agglutination reactions occurred in only 4 (9.5%) urine specimens and in no cerebrospinal fluid or serum specimens. The 94.7% sensitivity and specificity of the Directigen GBS test indicate that it is a useful reagent for the rapid diagnosis of invasive GBS infection in young infants.

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Comparison of slide coagglutination test and countercurrent immunoelectrophoresis for detection of group B streptococcal antigen in cerebrospinal fluid from infants with meningitis

Cited by 21 publications

References 8 publications

Direct Testing of Blood Cultures for Detection of Streptococcal Antigens

Direct Testing of Blood Cultures for Detection of Streptococcal Antigens

Staphylococcal Protein‐a Agglutination Assay for Avian Viruses

Detection of group B streptococcal antigen in body fluids by a latex-coupled monoclonal antibody assay

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