Comparison of slow freezing and vitrification methods for Venda cockerel’s spermatozoa

Mphaphathi, M. L.; Luseba, Dibungi; Nedambale, T. L.

doi:10.4236/ojas.2012.23028

Cited by 15 publications

(23 citation statements)

References 26 publications

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“…This was illustrated by a higher percentage of motile spermatozoa and viable spermatozoa with intact acrosomes and active mitochondria. This scenario agrees with previous studies that have reported better outcomes when sperm was preserved by slow freezing compared with those subjected to rapid cooling rates, as observed in several species, including turkeys (Meleagaris gallopavo), sandhill cranes (Grus canadensis), and roosters (Gallus gallus domesticus) [14,16]. Ultrarapid freezing in pellets might produce intracellular vitrification by drastically accelerating the cooling process, preventing the formation of intracellular ice crystals that can damage cells during the freezing/thawing process.…”

Section: Discussionsupporting

confidence: 91%

“…This is in agreement with the study by Santiago-Moreno et al [19], in which the thawing conditions had no influence on sperm quality in the gentoo penguin (Pygoscelis papua). However, in other species, such as black-footed penguins (Spheniscus demersus) and roosters, higher thawing temperatures had a more detrimental effect on spermatozoa [14,19,20]. Therefore, it seems that the thawing temperature may affect sperm quality characteristics in a species-specific manner.…”

Section: Discussionmentioning

confidence: 99%

“…Since the present research study was performed over a long period of time, the freezing protocol used in this experiment differed from the previous one. For this experiment, straws were frozen in a controlled-rate freezer (ICECUBE 1810 Sy-Lab), using the following two-step freezing ramp described by [14]: first, the temperature was decreased from −5 to −35 • C at a rate of 7 • C/min; then, it was decreased from −35 • C to −140 • C at 60 • C/min. The straws were then thawed at 37 • C for 30 s. The cryoprotectant that presented the best results was chosen in the subsequent experiment (2B), in which half of the ejaculates were extended with 4% of DMSO and the other half with 8%.…”

Section: Experimental Designmentioning

confidence: 99%

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Optimization of Sperm Cryopreservation Protocol for Peregrine Falcon (Falco peregrinus)

Cardoso

Sánchez-Ajofrín

Castaño³

et al. 2020

Animals

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Simple Summary: The process of semen cryopreservation can have multiple advantages in an ex situ conservation program. However, there is a necessity to adapt the protocol to the specificity of each species. With that in mind, we aimed to optimize the sperm freezing/thawing process and study the effect of different cryoprotectants in the peregrine falcon.Abstract: Sperm cryopreservation is a complex process that needs to be adapted to wild and domestic avian species to ensure proper efficiency. Because of its accessibility, the peregrine falcon may be used as a good model for studying other raptor species. To find the most optimal cryopreservation protocol for peregrine falcon ejaculates, sperm parameters such as motility, viability, DNA fragmentation, acrosome integrity, and mitochondrial activity were analyzed under different conditions by varying the freezing method (slow freezing in straws vs. ultrarapid freezing in pellets), thawing conditions (37 • C for 30 s vs. 5 • C for 1 min), type of cryoprotectant (DMA vs. DMSO), and concentration of DMSO (4% vs. 8%). Results show that slow cryopreservation in straws yielded greater percentages (p < 0.05) of motile spermatozoa (22.5% ± 4.4% vs. 0.0% ± 4.1%), viable spermatozoa with intact acrosomes (84.6% ± 4.3% vs. 77.4% ± 4.3%), and spermatozoa with active mitochondria (41.0% ± 6.7% vs.12.8% ± 6.7%), compared with those obtained by the ultrarapid freezing in pellets. However, no differences were found between different thawing conditions. Moreover, all sperm motility parameters were greater (p < 0.05) when DMSO was used during freezing compared with DMA, although the use of 3% and 8% DMSO produced similar results. In conclusion, these results represent important progress in the study of falcon semen cryopreservation protocol, highlighting the crucial steps of the process and the most suitable conditions. Animals 2020, 10, 691 2 of 11 existent species. A viable presented solution is the creation of a germplasm bank that contains the sperm, oocytes, or even embryos of the largest possible number of species [2,3].Since the storage of eggs or embryos from birds poses a difficult challenge because of their large size and complexity, sperm cells are a good alternative [4]. Sperm is fairly easy and inexpensive to acquire, and its cryopreservation is much more accessible compared with the oocyte/embryo, because of the small volume of water inside of the sperm [5]. Furthermore, existing technologies allow the semen of many animal species to be collected and used [6]. Frozen semen can be a very important part of ex situ conservation efforts, since it allows for the storage of genetic material from multiple species for a long period of time. Stored semen can be used in reintroduction projects or to enlarge the genetic pool of a fragmented population. It also allows for the selection of individuals of genetic interest and the exchange of samples between distant locations [7,8]. In birds, frozen semen could become an increasingly relevant tool, because captive breeding programs a...

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Section: Discussionsupporting

confidence: 91%

Section: Discussionmentioning

confidence: 99%

Section: Experimental Designmentioning

confidence: 99%

See 1 more Smart Citation

Optimization of Sperm Cryopreservation Protocol for Peregrine Falcon (Falco peregrinus)

Cardoso

Sánchez-Ajofrín

Castaño³

et al. 2020

Animals

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“…In slow freezing, the temperature is gradually reduced in a stepwise manner during cryopreservation process (Mphaphathi, Luseba, Sutherland, & Nedambale, 2012). The procedure was carried out as described by Tarig et al (2017).…”

Section: Slow Freezingmentioning

confidence: 99%

“…The rapid freezing or vitrification involves a swift speed of temperature conduction which provides a significant increase in cooling rate (Mphaphathi et al, 2012). The procedure consisting of different cryoprotectants was carried out on semen samples diluted with fraction 1 solution for the respective treatments as described by Srirattana et al (2013) with some modifications.…”

Section: Rapid Freezingmentioning

confidence: 99%

Comparative effect of slow and rapid freezing on sperm functional attributes and oxidative stress parameters of goat spermatozoa cryopreserved with tiger nut milk extender

Igbokwe

Iyasere

Sobayo³

et al. 2019

Reprod Domestic Animals

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Contents Comparative effect of slow and rapid freezing on sperm functional attributes and oxidative stress parameters of goat spermatozoa cryopreserved with tiger nut milk (TNM) extender was examined in this study. Pooled semen samples obtained from West African Dwarf (WAD) goat bucks were diluted with Tris‐based extenders containing different levels of TNM (0, 5, 10, 15 and 20 ml/100 ml extender). The diluted semen samples were subjected to slow and rapid freezing for a period of 7 days and thereafter evaluated for sperm functional attributes (percentage motility, acrosome integrity, membrane integrity, abnormality and livability) and oxidative stress (malondialdehyde [MDA] concentration and acrosin activity) parameters. Results showed that higher (p < 0.05) motility, livability, membrane and acrosome integrities in semen cryopreserved with slow freezing compared to rapid freezing. These parameters (motility, livability and membrane integrity) were higher (p < 0.05) in semen cryopreserved with 15% TNM in both slow and rapid freezing protocols. The results revealed that semen cryopreserved in slow freezing had lower (p < 0.05) abnormality compared to rapid freezing. Acrosin activity was higher in slow freezing compared to rapid freezing. Acrosin activity was higher at 15% TNM in both slow and rapid freezing. Lower (p < 0.05) MDA concentration was observed in semen cryopreserved using slow freezing compared to rapid freezing. The findings revealed improved post‐thaw sperm functional attributes and oxidative stress parameters of WAD goat spermatozoa cryopreserved with 15% TNM using slow freezing.

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Reproductive technologies in avian species

Barna¹,

Végi²,

Liptói³

et al. 2020

Reproductive Technologies in Animals

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Comparison of slow freezing and vitrification methods for Venda cockerel’s spermatozoa

Cited by 15 publications

References 26 publications

Optimization of Sperm Cryopreservation Protocol for Peregrine Falcon (Falco peregrinus)

Optimization of Sperm Cryopreservation Protocol for Peregrine Falcon (Falco peregrinus)

Comparative effect of slow and rapid freezing on sperm functional attributes and oxidative stress parameters of goat spermatozoa cryopreserved with tiger nut milk extender

Reproductive technologies in avian species

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