Comparison of small RNA profiles in Nicotiana benthamiana and Solanum lycopersicum infected by polygonum ringspot tospovirus reveals host-specific responses to viral infection

Margaria, Paolo; Miozzi, Laura; Ciuffo, M.; Rosa, Cristina; Axtell, Michael J.; Pappu, H. R.; Turina, Massimo

doi:10.1016/j.virusres.2015.09.019

Cited by 23 publications

(25 citation statements)

References 55 publications

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“…In addition, the highest and lowest mapping frequencies were observed for the S RNA and L RNA, respectively, based on the analyses of the GCFSV-infected transcriptomic reads (Fig 1B). A similar phenomenon has been reported in previous studies in which deep sequencing of viral siRNAs was conducted for the genome sequencing of TSWV and PolRSV [14,15]. Northern blotting to detect the quantities of S RNA and its corresponding siRNA molecules in a TSWV-infected tomato verified the findings obtained from the siRNA analyses [14].…”

Section: Discussionsupporting

confidence: 83%

“…During viral infection, small interfering RNAs (siRNAs) corresponding to the viral genome form a proportion of the small RNA population in infected plant tissues. Using NGS for the deep sequencing of siRNAs, tospoviruses such as TSWV, Capsicum chlorosis virus (CaCV) and Polygonum ringspot virus (PolRSV) can be diagnosed from diseased plant tissues [14, 15]. The whole-genome sequence of Chrysanthemum stem necrosis virus (CSNV) can be rapidly completed using v-RNA through NGS [16].…”

Section: Introductionmentioning

confidence: 99%

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Characterization of the genome of a phylogenetically distinct tospovirus and its interactions with the local lesion-induced host Chenopodium quinoa by whole-transcriptome analyses

et al. 2017

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Chenopodium quinoa is a natural local lesion host of numerous plant viruses, including tospoviruses (family Bunyaviridae). Groundnut chlorotic fan-spot tospovirus (GCFSV) has been shown to consistently induce local lesions on the leaves of C. quinoa 4 days post-inoculation (dpi). To reveal the whole genome of GCFSV and its interactions with C. quinoa, RNA-seq was performed to determine the transcriptome profiles of C. quinoa leaves. The high-throughput reads from infected C. quinoa leaves were used to identify the whole genome sequence of GCFSV and its single nucleotide polymorphisms. Our results indicated that GCFSV is a phylogenetically distinct tospovirus. Moreover, 27,170 coding and 29,563 non-coding sequences of C. quinoa were identified through de novo assembly, mixing reads from mock and infected samples. Several key genes involved in the modulation of hypersensitive response (HR) were identified. The expression levels of 4,893 deduced complete genes annotated using the Arabidopsis genome indicated that several HR-related orthologues of pathogenesis-related proteins, transcription factors, mitogen-activated protein kinases, and defense proteins were significantly expressed in leaves that formed local lesions. Here, we also provide new insights into the replication progression of a tospovirus and the molecular regulation of the C. quinoa response to virus infection.

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Section: Discussionsupporting

confidence: 83%

Section: Introductionmentioning

confidence: 99%

Characterization of the genome of a phylogenetically distinct tospovirus and its interactions with the local lesion-induced host Chenopodium quinoa by whole-transcriptome analyses

et al. 2017

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“…Examination of GFP-derived siRNAs may provide additional evidence for CaCV NSs effects on siRNA accumulation. Previously, NSs of TSWV, GRSV, PoLRSV, and TYRV were shown to suppress long-distance systemic RNA silencing (Hedil et al, 2015; Margaria et al, 2016). These authors suggest that NSs may interfere with biogenesis of the systemic silencing signal (secondary siRNAs).…”

Section: Discussionmentioning

confidence: 99%

Intracellular Localization, Interactions and Functions of Capsicum Chlorosis Virus Proteins

Gamage

Dietzgen

2017

Front. Microbiol.

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Tospoviruses are among the most devastating viruses of horticultural and field crops. Capsicum chlorosis virus (CaCV) has emerged as an important pathogen of capsicum and tomato in Australia and South-east Asia. Present knowledge about CaCV protein functions in host cells is lacking. We determined intracellular localization and interactions of CaCV proteins by live plant cell imaging to gain insight into the associations of viral proteins during infection. Proteins were transiently expressed as fusions to autofluorescent proteins in leaf epidermal cells of Nicotiana benthamiana and capsicum. All viral proteins localized at least partially in the cell periphery suggestive of cytoplasmic replication and assembly of CaCV. Nucleocapsid (N) and non-structural movement (NSm) proteins localized exclusively in the cell periphery, while non-structural suppressor of silencing (NSs) protein and Gc and Gn glycoproteins accumulated in both the cell periphery and the nucleus. Nuclear localization of CaCV Gn and NSs is unique among tospoviruses. We validated nuclear localization of NSs by immunofluorescence in protoplasts. Bimolecular fluorescence complementation showed self-interactions of CaCV N, NSs and NSm, and heterotypic interactions of N with NSs and Gn. All interactions occurred in the cytoplasm, except NSs self-interaction was exclusively nuclear. Interactions of a tospoviral NSs protein with itself and with N had not been reported previously. Functionally, CaCV NSs showed strong local and systemic RNA silencing suppressor activity and appears to delay short-distance spread of silencing signal. Cell-to-cell movement activity of NSm was demonstrated by trans-complementation of a movement-defective tobamovirus replicon. CaCV NSm localized at plasmodesmata and its transient expression led to the formation of tubular structures that protruded from protoplasts. The D155 residue in the 30K-like movement protein-specific LxD/N50-70G motif of NSm was critical for plasmodesmata localization and movement activity. Compared to other tospoviruses, CaCV proteins have both conserved and unique properties in terms of in planta localization, interactions and protein functions which will effect viral multiplication and movement in host plants.

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“…Indeed, and interestingly, a recent work with tospovirus polygonum ringspot virus (PolRSV), showed that the pool of viral-derived siRNAs mapping to the IGRs of its M and S-RNA segments are under-represented, including the S-RNA IGR which in the case of PolRSV is not predicted to fold into a hairpin structure (Margaria et al, 2015b). In respect to this, it is interesting to highlight that suppression of systemic silencing (and not local silencing) is a strategy that has been analysed and identified with RSS proteins from other plant viruses as well.…”

Section: Tospoviral Inducers Of Rna Silencing and Their Sequestrationmentioning

confidence: 99%

Tospovirus : induction and suppression of RNA silencing

Hedil¹

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Comparison of small RNA profiles in Nicotiana benthamiana and Solanum lycopersicum infected by polygonum ringspot tospovirus reveals host-specific responses to viral infection

Cited by 23 publications

References 55 publications

Characterization of the genome of a phylogenetically distinct tospovirus and its interactions with the local lesion-induced host Chenopodium quinoa by whole-transcriptome analyses

Characterization of the genome of a phylogenetically distinct tospovirus and its interactions with the local lesion-induced host Chenopodium quinoa by whole-transcriptome analyses

Intracellular Localization, Interactions and Functions of Capsicum Chlorosis Virus Proteins

Tospovirus : induction and suppression of RNA silencing

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