Comparison of small-scale methods for the rapid extraction of plant DNA suitable for PCR analysis

Rogers, Hilary J.; Burns, Nigel A.; Parkes, Helen C.

doi:10.1007/bf02684906

Cited by 17 publications

(10 citation statements)

References 13 publications

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“…Microsatellites were amplified from leaf extract following a modified version of the protocol presented by Wang et al . (1993) and tested by Rogers et al . (1996).…”

Section: Methodsmentioning

confidence: 99%

Cirsium species show disparity in patterns of genetic variation at their range‐edge, despite similar patterns of reproduction and isolation

2003

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Summary• Genetic variation was assessed across the UK geographical range of Cirsium acaule and Cirsium heterophyllum . A decline in genetic diversity and increase in population divergence approaching the range edge of these species was predicted based on parallel declines in population density and seed production reported seperately. Patterns were compared with UK populations of the widespread Cirsium arvense .• Populations were sampled along a latitudinal transect in the UK and genetic variation assessed using microsatellite markers.• Cirsium acaule shows strong isolation by distance, a significant decline in diversity and an increase in divergence among range-edge populations. Geographical structure is also evident in C. arvense , whereas no such patterns are seen in C. heterophyllum.• There is a major disparity between patterns of genetic variation in C. acaule and C. heterophyllum despite very similar patterns in seed production and population isolation in these species. This suggests it may be misleading to make assumptions about the geographical structure of genetic variation within species based solely on the present-day reproduction and distribution of populations.

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“…Microsatellites were amplified from leaf extract following a modified version of the protocol presented by Wang et al . (1993) and tested by Rogers et al . (1996).…”

Section: Methodsmentioning

confidence: 99%

Cirsium species show disparity in patterns of genetic variation at their range‐edge, despite similar patterns of reproduction and isolation

2003

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“…We extracted genomic DNA from the dried samples using an isopropanol precipitation method (Rogers et al. ), and characterized samples at eight microsatellite loci previously isolated from B. rapa or close relatives (Suwabe et al. ; Lowe et al.…”

Section: Methodsmentioning

confidence: 99%

The causes of selection on flowering time through male fitness in a hermaphroditic annual plant

Austen

Weis

2015

Evolution

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Flowering is a key life-history event whose timing almost certainly affects both male and female fitness, but tests of selection on flowering time through male fitness are few. Such selection may arise from direct effects of flowering time, and indirect effects through covariance between flowering time and the environment experienced during reproduction. To isolate these intrinsically correlated associations, we staggered planting dates of Brassica rapa families with known flowering times, creating populations in which age at flowering (i.e., flowering time genotype) and Julian date of flowering (i.e., flowering time environment) were positively, negatively, or uncorrelated. Genetic paternity analysis revealed that male fitness was not strongly influenced by seasonal environmental changes. Instead, when age and date were uncorrelated, selection through male fitness strongly favored young age at flowering. Strategic sampling offspring for paternity analysis rejected covariance between sire age at flowering and dam quality as the cause of this selection. Results instead suggest a negative association between age at flowering and pollen competitive ability. The manipulation also revealed that, at least in B. rapa, the often-observed correlation between flowering time and flowering duration is environmental, not genetic, in origin.

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“…Choice of DNA isolation method depends upon the type of sample in respect to purity and yield of the DNA obtainable. Several methods have been described [23][24][25] and numerous commercial kits are available for DNA isolation from plant tissues, based on different methodologies. Most of these methods have been optimized for DNA isolation from plant tissues but in this study we have developed a novel method which is able to isolate genomic DNA of bacteria, infesting potato tubers.…”

Section: Pcr Amplificationmentioning

confidence: 99%

Rapid Method for Isolation of PCR Amplifiable Genomic DNA of <i>Ralstonia solanacearum</i> Infested in Potato Tubers

Grover¹,

Chakrabarti²,

Azmi³

et al. 2012

AiM

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The aim of the present study was to develop a very fast and simple genomic DNA isolation method for Ralstonia solanacearum which infest potato tubers. One hundred potato tubers were collected and ten composite samples were prepared having 10 tubers each. Four different DNA isolation methods were used for bacterial genomic DNA isolation present in tubers. PCR with R. solanacearum specific primers and pathogenicity tests were performed. Out of four methods two gave PCR amplifiable DNA. The simplest method was boiling the cell lysate for 5 min, vortexing for 2 min then extraction with phenol chloroform method. This method provides significant amount of DNA which is free from contaminants thus rendering the DNA amicable to PCR amplification. The developed method would be useful for quick and sensitive detection of this pathogen in seed potatoes and would be beneficial to stop the further spread of pathogen.

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Comparison of small-scale methods for the rapid extraction of plant DNA suitable for PCR analysis

Cited by 17 publications

References 13 publications

Cirsium species show disparity in patterns of genetic variation at their range‐edge, despite similar patterns of reproduction and isolation

Cirsium species show disparity in patterns of genetic variation at their range‐edge, despite similar patterns of reproduction and isolation

The causes of selection on flowering time through male fitness in a hermaphroditic annual plant

Rapid Method for Isolation of PCR Amplifiable Genomic DNA of <i>Ralstonia solanacearum</i> Infested in Potato Tubers

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Comparison of small-scale methods for the rapid extraction of plant DNA suitable for PCR analysis

Cited by 17 publications

References 13 publications

Cirsium species show disparity in patterns of genetic variation at their range‐edge, despite similar patterns of reproduction and isolation

Cirsium species show disparity in patterns of genetic variation at their range‐edge, despite similar patterns of reproduction and isolation

The causes of selection on flowering time through male fitness in a hermaphroditic annual plant

Rapid Method for Isolation of PCR Amplifiable Genomic DNA of &lt;i&gt;Ralstonia solanacearum&lt;/i&gt; Infested in Potato Tubers

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Product

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About

Rapid Method for Isolation of PCR Amplifiable Genomic DNA of <i>Ralstonia solanacearum</i> Infested in Potato Tubers