Laboratory data (thermal fractionation, pepsin extraction, C:N o ratio) of dung and manure were mutually compared and contrasted with plantavailability of organic N (N o ) as found in a greenhouse experiment according to the double-pot technique. Two types of fresh cow dung (one with a relatively wide and the other with a relatively narrow C:N o ratio) and four types of manure (from poultry, sheep, pigs and cow) were compared with ammonium nitrate as chemical reference fertilizer. Relative effectiveness of organic N (RE o ) was used as characteristic; it was calculated as the fraction of organic N that has the same availability to plants as inorganic N. RE o for poultry and sheep manure could not be assessed, probably because of NH 3 volatilization causing direct damage to plants and N losses. RE o values decreased in the order: dung with narrow C:N o [ dung with wide C:N o [ pig manure [ cow manure. Thermal fractionation did not provide a suitable index of plant-availability of organic N. Pepsin extracted organic N gave a positive, and C:N o ratio a negative relationship with RE o . Also between pepsin extracted organic N and C:N o ratio a negative relationship was found. As C:N o ratio is relatively easy to determine, it is considered the most practical laboratory index for plant availability of organic N in animal manures low in ammonia. When using the double-pot technique, application rates of manure types high in ammonia should be restricted.