Comparison of SPIO and USPIO for in Vitro Labeling of Human Monocytes: MR Detection and Cell Function

Engberink, Raoul D. Oude; Pol, Susanne M. A. van der; Döpp, E. A.; Vries, Helga E. de; Blezer, Erwin L. A.

doi:10.1148/radiol.2432060120

Cited by 117 publications

(113 citation statements)

References 27 publications

Supporting

Mentioning

106

Contrasting

Order By: Relevance

“…Although MPIO was excluded from all of these studies, it was found that phagocytic monocytes are more effectively labeled with SSPIO (150 nm) compared with USPIO (30 nm) [18,29]. Further, it was found that ionic carboxydextran-coated SSPIO (i.e.…”

Section: Introductionmentioning

confidence: 99%

Size, charge and concentration dependent uptake of iron oxide particles by non-phagocytic cells

2008

View full text Add to dashboard Cite

A promising new direction for contrast-enhanced magnetic resonance (MR) imaging involves tracking the migration and biodistribution of superparamagnetic iron oxide (SPIO)-labeled cells in vivo. Despite the large number of cell labeling studies that have been performed with SPIO particles of differing size and surface charge, it remains unclear which SPIO configuration provides optimal contrast in non-phagocytic cells. This is largely because contradictory findings have stemmed from the variability and imprecise control over surface charge, the general need and complexity of transfection and/or targeting agents, and the limited number of particle configurations examined in any given study. In the present study, we systematically evaluated the cellular uptake of SPIO in non-phagocytic T cells over a continuum of particle sizes ranging from 33 nm to nearly 1.5 μm, with precisely controlled surface properties, and without the need for transfection agents. SPIO labeling of T cells was analyzed by flow cytometry and contrast enhancement was determined by relaxometry. SPIO uptake was dose dependent and exhibited sigmoidal charge dependence, which was shown to saturate at different levels of functionalization. Efficient labeling of cells was observed for particles up to 300nm, however micron-sized particle uptake was limited. Our results show that an unconventional highly cationic particle configuration at 107 nm maximized MR contrast of T cells, outperforming the widely utilized USPIO (<50 nm).

show abstract

Section: Introductionmentioning

confidence: 99%

Size, charge and concentration dependent uptake of iron oxide particles by non-phagocytic cells

2008

View full text Add to dashboard Cite

show abstract

“…19 Particles with larger diameters were more efficiently incorporated into macrophages. 21,22 istration, there were no differences in labeling efficiencies between ATDM at 28 nm and at 74 nm ( Figure 3A).…”

Section: Discussionmentioning

confidence: 97%

“…All USPIO particles were dissolved in the cell growth medium at a concentration of 0.3 mg Fe/mL, which is similar to the concentration used in another published report. 18 Cells (2 × 10 6 ) were incubated with medium containing USPIO particles for 1, 2, or 4 h (same time points noted in a previous report) 19 at 37°C under 5% CO 2 . After incubation, the medium was removed by two washes with phosphatebuffered saline (PBS) followed by fixation of the cells for 30 min with 7.5% formalin.…”

Section: Measurement Of Uspio-labeling Efficiencymentioning

confidence: 99%

Impact of surface coating and particle size on the uptake of small and ultrasmall superparamagnetic iron oxide nanoparticles by macrophages

Saito¹,

Tsugeno²,

Koto³

et al. 2012

IJN

View full text Add to dashboard Cite

Purpose: Magnetic resonance imaging (MRI) using contrast agents like superparamagnetic iron oxide (SPIO) is an extremely versatile technique to diagnose diseases and to monitor treatment. This study tested the relative importance of particle size and surface coating for the optimization of MRI contrast and labeling efficiency of macrophages migrating to remote inflammation sites. Materials and methods:We tested four SPIO and ultrasmall superparamagnetic iron oxide (USPIO), alkali-treated dextran magnetite (ATDM) with particle sizes of 28 and 74 nm, and carboxymethyl dextran magnetite (CMDM) with particle sizes of 28 and 72 nm. Mouse macrophage RAW264 cells were incubated with SPIOs and USPIOs, and the labeling efficiency of the cells was determined by the percentage of Berlin blue-stained cells and by measuring T 2 relaxation times with 11.7-T MRI. We used trypan blue staining to measure cell viability. Results: Analysis of the properties of the nanoparticles revealed that ATDM-coated 74 nm particles have a lower T 2 relaxation time than the others, translating into a higher ability of MRI negative contrast agent. Among the other three candidates, CMDM-coated particles showed the highest T 2 relaxation time once internalized by macrophages. Regarding labeling efficiency, ATDM coating resulted in a cellular uptake higher than CMDM coating, independent of nanoparticle size. None of these particle formulations affected macrophage viability. Conclusion: This study suggests that coating is more critical than size to optimize the SPIO labeling of macrophages. Among the formulations tested in this study, the best MRI contrast and labeling efficiency are expected with ATDM-coated 74 nm nanoparticles.

show abstract

“…Several groups have investigated the effects of particle size and iron concentration on SPIO and USPIO uptake rates. In comparison with USPIOs, macrophages and monocytes appear to have much a higher uptake preference for SPIOs and are more effectively labeled by the latter [76,77]. Thorek et al [78] tested the uptake rate of different sized SPIOs in non-phagocytic T-cells and showed that particles <300 nm were most efficient in cell labeling.…”

Section: Fig 5 Cd8mentioning

confidence: 99%

MRI in Rodent Models of Brain Disorders

et al. 2011

View full text Add to dashboard Cite

Summary: Magnetic resonance imaging (MRI) is a wellestablished tool in clinical practice and research on human neurological disorders. Translational MRI research utilizing rodent models of central nervous system (CNS) diseases is becoming popular with the increased availability of dedicated small animal MRI systems. Projects utilizing this technology typically fall into one of two categories: 1) true "pre-clinical" studies involving the use of MRI as a noninvasive disease monitoring tool which serves as a biomarker for selected aspects of the disease and 2) studies investigating the pathomechanism of known human MRI findings in CNS disease models. Most small animal MRI systems operate at 4.7-11.7 Tesla field strengths. Although the higher field strength clearly results in a higher signalto-noise ratio, which enables higher resolution acquisition, a variety of artifacts and limitations related to the specific absorption rate represent significant challenges in these experiments. In addition to standard T1-, T2-, and T2*-weighted MRI methods, all of the currently available advanced MRI techniques have been utilized in experimental animals, including diffusion, perfusion, and susceptibility weighted imaging, functional magnetic resonance imaging, chemical shift imaging, heteronuclear imaging, and 1 H or 31 P MR spectroscopy. Selected MRI techniques are also exclusively utilized in experimental research, including manganese-enhanced MRI, and cell-specific/molecular imaging techniques utilizing negative contrast materials. In this review, we describe technical and practical aspects of small animal MRI and provide examples of different MRI techniques in anatomical imaging and tract tracing as well as several models of neurological disorders, including inflammatory, neurodegenerative, vascular, and traumatic brain and spinal cord injury models, and neoplastic diseases.

show abstract

Comparison of SPIO and USPIO for in Vitro Labeling of Human Monocytes: MR Detection and Cell Function

Abstract: In vitro labeling of human monocytes is effective by using SPIOs, not USPIOs. Incubation with SPIOs (1.0 mg Fe/mL) results in efficient labeling detectable on MR images and does not affect cellular viability and activation markers such as cell migration and cytokine production.

Cited by 117 publications

References 27 publications

Size, charge and concentration dependent uptake of iron oxide particles by non-phagocytic cells

Size, charge and concentration dependent uptake of iron oxide particles by non-phagocytic cells

Impact of surface coating and particle size on the uptake of small and ultrasmall superparamagnetic iron oxide nanoparticles by macrophages

MRI in Rodent Models of Brain Disorders

Contact Info

Product

Resources

About