Comparison of Staphylococcus aureus isolates associated with food intoxication with isolates from human nasal carriers and human infections

Wattinger, Lilian; Stephan, Roger; Layer, Franziska; Johler, Sophia

doi:10.1007/s10096-011-1330-y

Cited by 75 publications

(79 citation statements)

References 30 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Spa typing assigned these strains to t018, a spa type previously found in common methicillin-resistant S. aureus clones in the United Kingdom and Denmark (Bartels et al, 2009;Khandavilli et al, 2009), as well as S. aureus strains associated with nasal colonization, infections, and food poisoning (Wattinger et al, 2012). These seven highly similar isolates originated from food samples, nasal swabs of the caterer, as well as a stool sample from a patient.…”

Section: Discussionmentioning

confidence: 91%

“…Total DNA was isolated using the Qiagen DNeasy kit (Hilden, Germany), following the manufacturer's instructions. The spa gene was amplified as previously described (Wattinger et al, 2012). Amplicons were purified and the concentration of nucleic acids was determined using a Nanodrop ND-UV/Vis spectrophotometer (NanoDrop Technologies, Wilmington, DE).…”

Section: Spa Typingmentioning

confidence: 99%

See 1 more Smart Citation

Outbreak of Staphylococcal Food Poisoning Due to SEA-ProducingStaphylococcus aureus

Johler

Tichaczek-Dischinger

Rau

et al. 2013

Foodborne Pathogens and Disease

View full text Add to dashboard Cite

In 2008, 150 people gathered for a wedding celebration in Baden-Württemberg, Germany. Three hours after ingestion of a variety of foods including pancakes filled with minced chicken, several guests exhibited symptoms of acute gastroenteritis such as vomiting, diarrhea, fever, and ague. Twelve guests were reported to have fallen ill, with nine of these seeking medical care in hospitals. At least four patients were admitted to the hospital and received inpatient treatment, among them a 2-year-old child and a woman in the 4th month of pregnancy. Within 24 h of the event, an investigative team collected a variety of samples including refrigerated leftovers, food in the storage unit of the caterer, nasal swabs of the caterer, as well as 21 environmental swabs. Five stool samples from patients were provided by the hospitals. Staphylococcus aureus isolates were gathered from eight samples, among them nasal swabs of the caterer, food samples, and one stool sample. Fourier transform-infrared spectroscopy was used for species identification and for primary clustering of the isolates in a similarity tree. The isolates were further characterized by spa typing and pulsed-field gel electrophoresis, and a DNA microarray was used to determine the presence/absence of genes involved in virulence and antimicrobial resistance. We were able to match an enterotoxigenic strain from the stool sample of a patient to isolates of the same strain obtained from food and the nasal cavity of a food handler. The strain produced the enterotoxin SEA and the toxic shock syndrome toxin-1, and was also found to exhibit the genes encoding enterotoxins SEG and SEI, as well as the enterotoxin gene cluster egc. This is one of only a few studies that were able to link a staphylococcal food poisoning outbreak to its source. In 2008, 150 people gathered for a wedding celebration in Baden-Wü rttemberg, Germany. Three hours after ingestion of a variety of foods including pancakes filled with minced chicken, several guests exhibited symptoms of acute gastroenteritis such as vomiting, diarrhea, fever, and ague. Twelve guests were reported to have fallen ill, with nine of these seeking medical care in hospitals. At least four patients were admitted to the hospital and received inpatient treatment, among them a 2-year-old child and a woman in the 4th month of pregnancy.Within 24 h of the event, an investigative team collected a variety of samples including refrigerated leftovers, food in the storage unit of the caterer, nasal swabs of the caterer, as well as 21 environmental swabs. Five stool samples from patients were provided by the hospitals. Staphylococcus aureus isolates were gathered from eight samples, among them nasal swabs of the caterer, food samples, and one stool sample. Fourier transforminfrared spectroscopy was used for species identification and for primary clustering of the isolates in a similarity tree. The isolates were further characterized by spa typing and pulsed-field gel electrophoresis, and a DNA microarray was used to determin...

show abstract

Section: Discussionmentioning

confidence: 91%

Section: Spa Typingmentioning

confidence: 99%

Outbreak of Staphylococcal Food Poisoning Due to SEA-ProducingStaphylococcus aureus

Johler

Tichaczek-Dischinger

Rau

et al. 2013

Foodborne Pathogens and Disease

View full text Add to dashboard Cite

show abstract

“…All clonal complexes detected in this study can be commonly found among S. aureus strains isolated from humans colonized or infected with S. aureus [13,21,22] . Furthermore, there is evidence that the main reservoir of the S. aureus isolates is not the clinical setting, due to the predominance of MLST CC30 and CC15, described as community-associated S. aureus [23].…”

Section: Discussionmentioning

confidence: 99%

“…In order to visualize the diversity of DNA microarray patterns, as well as strain relatedness, hybridization patterns were analysed using the SplitsTree software (www.splitstree.org), designed to compute unrooted phylogenetic networks from molecular sequence data [12]. DNA microarray gene profiles were converted to ‘sequence-like' strings of information as previously described [13]. …”

Section: Methodsmentioning

confidence: 99%

Genetic and Phenotypic Characteristics of <b><i>Staphylococcus aureus</i></b> Isolates from Cystic Fibrosis Patients in Austria

Masoud-Landgraf

Johler²,

Badura³

et al. 2015

Respiration

View full text Add to dashboard Cite

Background: Cystic fibrosis (CF) is the most common life-limiting inherited disease in Caucasian populations. While pathological changes can be seen in various organs, morbidity and mortality are mainly related to the respiratory tract, with patients suffering from chronic bronchopulmonary infections with characteristic pathogens including Staphylococcus aureus.Objectives: To date, there is only very limited data on the genetic and phenotypic characteristics of S. aureus in CF patients. Therefore, in our study, we characterized 58 S. aureus isolates collected from CF patients in Austria by spa typing, DNA microarray profiling, as well as antimicrobial susceptibility testing in order to determine common genomic and antimicrobial resistance features. The tested strain collection exhibited high genomic diversity. Results: The 58 isolates were assigned to 16 clonal complexes and 48 spa types and differed greatly regarding their virulence and resistance gene profiles. The predominant clonal complexes were MLST CC30 (22%), CC15 (16%), CC45 (14%), and CC5 (12%), complexes that are highly prevalent worldwide among S. aureus strains isolated from humans colonized or infected with S. aureus. DNA microarray profiles showed a wide variety of genes encoding antimicrobial resistance and virulence factors such as various leukocidins, haemolysins, enterotoxins, exfoliative toxins, toxic shock syndrome toxin, as well as genes involved in adhesion and immune evasion. Conclusions: While a large number of strains exhibited resistance to one or several antimicrobial agents, methicillin-resistant S. aureus was found at a low prevalence of 3% (n = 2) only. The two methicillin-resistant S. aureus isolates were assigned to CC152/t355 (SCCmecV) and CC5/t001 (SCCmecI). This is the first study to genetically characterize S. aureus isolates in CF patients in Austria.

show abstract

“…S. aureus is one of the most commonly identified causes of food poisoning due to the ability of enterotoxigenic strains to preform staphylococcal enterotoxins (SEs) in food (Di Giannatale, Prencipe, Tonelli, Marfoglia, & Migliorati, 2011;Fetsch et al, 2014). S. aureus colonization in humans can cause serious infections, toxinoses and life-threatening diseases, such as skin and soft tissue infections, toxic shock syndrome and septicemia (Wattinger, Stephan, Layer, & Johler, 2012). Due to these serious threats, safe and natural food preservatives that inhibit bacterial and fungal growth have become a major priority in the past decades (Badaoui Najjar, Kashtanov, & Chikindas, 2007).…”

Section: Introductionmentioning

confidence: 99%

Analysis of the gene expression profile of Staphylococcus aureus treated with nisin

et al. 2016

View full text Add to dashboard Cite

Comparison of Staphylococcus aureus isolates associated with food intoxication with isolates from human nasal carriers and human infections

Cited by 75 publications

References 30 publications

Outbreak of Staphylococcal Food Poisoning Due to SEA-ProducingStaphylococcus aureus

Outbreak of Staphylococcal Food Poisoning Due to SEA-ProducingStaphylococcus aureus

Genetic and Phenotypic Characteristics of <b><i>Staphylococcus aureus</i></b> Isolates from Cystic Fibrosis Patients in Austria

Analysis of the gene expression profile of Staphylococcus aureus treated with nisin

Contact Info

Product

Resources

About