Comparison of Subgingival Microbiota in Fluorosed and Non-fluorosed Chronic Periodontitis Patients

Abstract

ABSTRACT:Background: Periodontal disease comprises a group of inflammatory conditions of the supporting tissues of the teeth that are caused by bacteria. Only few species of subgingival microbiota have been identified as etiologic pathogens. The roots of teeth with mottled enamel are irregular and rough which favor plaque accumulation. The present study aimed at assessing the microbial composition of subgingival plaque in Fluorosed and Non Fluorosed Chronic Periodontitis subjects using PCR. Materials and Method: 40 chronic periodontitis patients were selected and divided into fluorosed and nonfluorosed groups. The Oral hygiene status, fluorosis status and periodontal status were recorded and compared. After recording the clinical parameters, at least 3 sites of 5-7 mm of pocket depth were selected for subgingival pooled plaque sample collection. Samples were subjected to culture and PCR analysis. Results: The PCR results for A. actinomycetemcomitans, P. gingivalis, T. forsythia and T. denticola organisms when compared between the groups showed nostatistically significant difference. But for T. forsythia and T. denticolastatistically significant difference was observed. Conclusion: The patients in fluorosed group showed more positive findings for periodontitis when compared to non-fluorosed group. Severity of periodontal disease was directly proportionate with the degree of fluorosis when compared within the fluorosed group. Qualitative comparison of subgingival microbiota showed no significant differences between the fluorosed and non fluorosed group. The specific detection rates of T. forsythia and T. denticola is more in the fluorosed group than the non fluorosed group.