Comparison of survival rates of day 7 and day 8 bovine embryos after fast freezing and thawing

Niemann, Heinrich; Lampeter, W.; Sacher, Bernhard; Kruff, B.

doi:10.1016/0093-691x(82)90166-2

Cited by 23 publications

(3 citation statements)

References 12 publications

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“…Others have reported similar results after transfer of bovine embryos produced in vivo . They observed a tendency for pregnancy rate to decrease with advancing maturity of the embryo (Coleman et al ., 1986) and better pregnancy rates after transfer of Bd7 (Niemann et al ., 1982). Our results agree with other studies that support the view that embryo quality is a more accurate predictor of success than the stage of embryonic development (Lindner & Wright, 1983).…”

Section: Discussionmentioning

confidence: 99%

DNA fragmentation, transgene expression and embryo development after intracytoplasmic injection of DNA–liposome complexes in IVF bovine zygotes

Vichera

Moro

Buemo

et al. 2012

Zygote

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Summary This study was designed to evaluate the quality and viability of bovine embryos produced by in vitro fertilization (IVF), after intracytoplasmic injection of pCX-EGFP-liposome complexes or pBCKIP2.8-liposome complexes (plasmids that codify the human insulin gene). Cleavage, blastocysts and expanded blastocysts rates of these both groups were not different from that of controls (IVF or IVF embryos injected with liposomes alone; IVF-L). The percentage of EGFP-positive (EGFP+) blastocysts was 41.8%. In Experiment 2, the blastocysts obtained after injection of pCX-EGFP-liposome complexes that did or did not express the transgene, were analyzed by TUNEL (terminal deoxynucleotidyl transferase dUTP nick-end labelling) assay at days 6, 7 and 8 of culture in vitro(Bd6, Bd7 and Bd8), in order to evaluate DNA fragmentation. The EGFP+ blastocysts showed different proportions of TUNEL-positive cells (T+) at Bd6, Bd7 and Bd8 (91, 73.7 and 99.5%, respectively) while blastocysts without EGFP expression (EGFP-) showed statistically lower numbers of fragmented nuclei (0, 44.6 and 85%, respectively; P < 0.05). There was no evidence of DNA fragmentation in either Bd6 or Bd7 IVF and IVF-L control blastocysts, but T+ nuclei were detected at Bd8 in both groups (66.4 and 85.8% respectively). Finally, IVF blastocysts (n = 21) injected with insulin-liposome complexes, cultured for 6, 7 and 8 days, were transferred to recipient cows. Pregnancy rates of 18.2% (2/11) and 40% (2/5) resulted from the transfer of Bd6 and Bd7 cells, respectively. Two pregnancies developed to term but they were not transgenic for the insulin gene. In conclusion, EGFP expression affects DNA integrity but not embryo development. Moreover, additional transfers are required in order to overcome the drawbacks generated by in vitro culture length and transgene expression.

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Section: Discussionmentioning

confidence: 99%

DNA fragmentation, transgene expression and embryo development after intracytoplasmic injection of DNA–liposome complexes in IVF bovine zygotes

Vichera

Moro

Buemo

et al. 2012

Zygote

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“…Massip and Mulnard (1980) showed that the structure between the trophoblast cells of embryos is damaged during freezing and thawing. Niemann et al (1982) suggested that damage to desmosomes between trophoblast cells explains the significantly lower posttransfer pregnancy rates for expanded blastocysts. Recently, Kuwayama et al (1992) observed an apparent difference in the degree of intramembrane particle aggregation in the plasma membrane of bovine blastocysts vitrified by two methods using a freeze replica technique.…”

Section: Discussionmentioning

confidence: 99%

“…In most studies, the quality or viability of the cryopreserved embryos has been evaluated by posttransfer pregnancy rates (Els-0 1994 WILEY-LISS, INC. den et al, 1982;Chupin et al, 1984). Morphological and, particularly, ultrastructural studies of frozenthawed bovine embryos have also been carried out (Massip and Mulnard, 1980;Niemann et al, 1982, but little is known about the effect of freezing on the morphology and viability of inner cell mass (ICM) cells in bovine embryos.…”

Section: Introductionmentioning

confidence: 99%

Effects of freezing of bovine preimplantation embryos derived from oocytes fertilized in vitro on survival of their inner cell mass cells

Iwasaki

Yoshikane

et al. 1994

Molecular Reproduction Devel

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The morphology of the inner cell mass (ICM) cells and the proportion of dead ICM cells in frozen-thawed bovine preimplantation embryos were investigated by differential fluorochrome staining. Embryos at the blastocyst stage of development were frozen and thawed by two different techniques (three-step and one-step) in two different basic salt solutions (PBS and TCM 199) containing 1.36M glycerol. After thawing and glycerol removal, embryos were co-cultured in a cumulus cells monolayer in TCM 199 for 48 hr (morula) or 24 hr (blastocysts). Differential cell counts of the ICM and trophectoderm were then done using differential fluorochrome staining. Overall, there was no significant difference in the viability of embryos frozen in the two basic salt solutions. Low proportions of dead ICM cells were observed in embryos frozen at the morula stage in both PBS (19.1%) or TCM 199 (18.0%). However, blastocyst stage embryos frozen by the three-step technique had a higher (P < 0.05) proportion of dead ICM cells in TCM 199 (37.7%) than in PBS (18.2%). Blastocysts frozen by the one-step technique had a higher (P < 0.05) proportion of dead ICM cells (42.2%) than those frozen by the three-step technique (18.2%), regardless of basic salt solutions. Results indicate that freezing and thawing damages ICM cells in morphologically normal embryos and that the degree of damage depended on the basic salt solution and the freezing method.

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Cryopreservation of Bovinein VitroProduced Embryos Using Ethylene Glycol in Controlled Freezing or Vitrification

1999

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Comparison of survival rates of day 7 and day 8 bovine embryos after fast freezing and thawing

Cited by 23 publications

References 12 publications

DNA fragmentation, transgene expression and embryo development after intracytoplasmic injection of DNA–liposome complexes in IVF bovine zygotes

DNA fragmentation, transgene expression and embryo development after intracytoplasmic injection of DNA–liposome complexes in IVF bovine zygotes

Effects of freezing of bovine preimplantation embryos derived from oocytes fertilized in vitro on survival of their inner cell mass cells

Cryopreservation of Bovinein VitroProduced Embryos Using Ethylene Glycol in Controlled Freezing or Vitrification

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