Comparison of systems combining auxins with thidiazuron or kinetin supplemented with polyvinylpirrolidone during embryogenic callus induction in three &lt;i&gt;Theobroma cacao&lt;/i&gt; L. genotypes

Kouassi, Modeste Kan; Koffi, Edmond; Silue, Oumar; Tahi, Mathias Gnion; Touré, M.; Konan, Eugène Pacôme Kouakou

doi:10.4314/ijbcs.v12i2.15

Cited by 5 publications

(7 citation statements)

References 17 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…No embryogenic callus and embryo production was obtained with 4.5 μM 2,4-D in the C8 genotype. This result is consistent with those from Kouassi et al, (2018) who used this same concentration for 2,4-D and 2,4,5-T. But a combination of 4.5 μM 2, 4-D with 20 μl of TDZ led to 2,20 of PEC in C8 genotype (Kouassi et al, 2017a).…”

Section: Pt Stsupporting

confidence: 89%

“…Genotypes coded C1 and C14 are from Côte d'Ivoire and responsive to somatic embryogenesis. But C14 was less responsive compared to C1 (Kouassi et al, 2017 a,b;2018). Methods Collection and disinfection of flower buds: In the morning before 9 am, about 4 to 5 mm long flower buds were collected and placed in jars and stored in a cooler containing ice and sent to the laboratory.…”

Section: Methodsmentioning

confidence: 99%

“…Elite genotypes are characterized by good genotypic performance (high productivity) and disease resistance. For example, the genotype coded C8 is an elite cocoa clone but its multiplication by somatic embryogenesis has given embryogenic callus at very low levels (Kouassi et al, 2017a(Kouassi et al, , 2018. The objective of the present study was to overcome the recalcitrance of genotypes which are of undeniable interest for the cocoa culture.…”

Section: Introductionmentioning

confidence: 98%

See 2 more Smart Citations

Induction of somatic embryos of recalcitrant genotypes of <i>Theobroma cacao L.</i>

Kone¹,

Modeste²,

Oulo³

et al. 2019

J. App. Bioscience.

View full text Add to dashboard Cite

Objective: In cocoa tree (Theobroma cacao L.), some elite genotypes have shown, in standard study conditions, an absence or a very weak response to induction of somatic embryos. This is the case of the original C8 genotype Trinidad. This study aims to improve the production of somatic embryos in this genotype Methodology and results: To do this, staminodes and petals excised from immature buds of genotypes C1, C8 and C14 were used. Genotypes C1 and C14 are embryogenic under standard conditions. These floral explants were cultured on induction media differing by the type and concentration of auxins. Callus induction obtained in the three studied genotypes ranged from 80% to 90% with the petal explants and from 70% to 80% with the staminodes regardless of the type and concentration of auxins. Transfer of callogenic explants to DKW (Driver & Kuniyuki, 1984) medium supplemented with sucrose and glucose allowed the induction of somatic embryos at mean rates varying from 5% to 20% after only 84 days with petal explants and media supplemented with 2,4-dichlorophenoxyacetic acid (2,4-D) and 2, 4, 5-trichlorophenoxyacetic acid (2,4,5-T). No induction of somatic embryos was observed on the control medium with the C1, C8 and C14 genotypes. The C1 genotype induced somatic embryos in the presence of all concentrations of auxins. The highest rate of embryogenic callus and somatic embryo induction was obtained with 18 μM of 2, 4, 5-T in all C1 genotypes (39.29 ± 0.28; 17.98 ± 0.10), C8 (36.29% ± 0.26 and 15.01 ± 0.07) and C14 (33.92% ± 0.26 and 14.50 ± 0.16). The 2, 4, 5-trichlorophenoxyacetic acid (2, 4, 5-T). at 18 μM is the most appropriate auxin to remove recalcitrance in cocoa genotype C8. The use of higher 18 μM concentrations of 2, 4, 5-trichlorophenoxyacetic acid (2, 4, 5-T). and its application to other recalcitrant genotypes could confirm its beneficial effect on the removal of recalcitrance to somatic embryogenesis in cocoa. Conclusion and application of results: 18 μM concentration of 2, 4, 5-trichlorophenoxyacetic acid (2, 4, 5-T) gave the best percentage of embryogenic explants and the highest average number of embryos in the three genotypes tested. Therefore, this protocol using 18 μM of 2, 4, 5-trichlorophenoxyacetic acid (2, 4, 5-T) could be used to overcome recalcitrance to somatic embryogenesis in cocoa.

show abstract

Section: Pt Stsupporting

confidence: 89%

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 98%

See 1 more Smart Citation

Induction of somatic embryos of recalcitrant genotypes of <i>Theobroma cacao L.</i>

Kone¹,

Modeste²,

Oulo³

et al. 2019

J. App. Bioscience.

View full text Add to dashboard Cite

show abstract

“…The elite genotypes such as C1, C8, C14, C15 and C16 are characterized by good genotypic performance (high productivity) and resistance to diseases. However, low levels of embryogenic calli genotypes C8 (0.03%), C14 (14.32%) (Kouassi et al, 2018) and C16 (8.85%) (Kouassi et al, 2017b) were obtained with somatic embryogenesis. Therefore, it is highly important to develop a protocol allowing maximum high embryo production even in recalcitrant genotypes.…”

Section: Introductionmentioning

confidence: 97%

Use of mineral salts to remove recalcitrance to somatic embryogenesis of improved genotypes of cacao (Theobrama cacao L.)

KonÃ¹,

Nâ€TMNan-Alla²,

Kouassi³

et al. 2021

Afr. J. Biotechnol.

View full text Add to dashboard Cite

Some improved genotypes of cocoa tree (Theobroma cacao L.), have shown in standard study conditions of absence or a very low response to the induction of somatic embryos. This is the case of the recalcitrant genotypes C8 and C15, partially recalcitrant genotypes C14 and C16. This study aims to improve the production of somatic embryos of recalcitrant genotypes. Staminodes and petals excised from the immature buds of the five genotypes C1, C8, C14, C15 and C16 were used as plant material. These floral explants were cultured on different media containing two type of mineral salts such as potassium sulphate (K 2 SO 4 ) at concentrations 18, 27, 36 and 45 mM and magnesium sulphate (MgSO 4 ) whose concentrations used are 5, 10, 15 and 20 mM. Calli induction was obtained in the five (05) genotypes at percentages ranging from 50 to 100% with staminodes and from 61 to 100% with petals on all media. The transfer of the callogenic explants on the developmental medium allowed the induction of embryogenic calli and somatic embryos after 84 days only with petal explants. The most important PEC and NSE were obtained with two mineral salts concentrations, 27 mM K 2 SO 4 and 15 mM MgSO 4 for all genotypes and varied to 20 to 40%. Potassium sulphate (K2SO4) at 27 and magnesium sulphate (MgSO 4 ) at 15 mM are the concentrations of the most suitable mineral salts to overcome recalcitrance of cocoa genotypes.

show abstract

“…Genotypes have variability in response to the protocols (Garcia et al, 2016). In previous work, research teams (Kouassi et al, 2018;Kahia et al, 2017;Garcia et al, 2016;Li et al, 1998) had conducted investigations with a specific protocol to question somatic embryogenic expression in T. cacao genotypes. This approach (use of a specific somatic embryogenesis protocol applied to different genotypes from a specific experimental area) resulted in specific genotypes and protocols used.…”

Section: Introductionmentioning

confidence: 99%

Preferential expression of somatic embryogenesis in five elite genotypes of Theobroma cacao (L.) associated with explant type and protocols used

N’goran,

Minyaka,

Kouassi

et al. 2023

Afr. J. Biotechnol.

View full text Add to dashboard Cite

This study aims to identify the suitable subculturing time, culture media (protocols) and explants type suitable for somatic embryogenesis expression in five elite genotypes of Theobroma cacao L. Thus, petals and staminodes explants extracted from immature flower buds of genotypes (C1, C8, C9, C14, and C16) are first grown in different media (CIM and CDM) to assess the subculturing time. Then, successive transfers in EDM medium at every 4 weeks intervals until 24 weeks. To assess culture media preference, these genotypes were grown in four different protocols (I, II, III, and IV) for callus induction. They were then successively subcultured (four times at 4 weeks intervals) into EDM. About the subculture time, embryo expression is earlier in C1, C9, C14, and C16 genotypes (2nd and 4th weeks) than C8 genotype (12 and 16th weeks). However, C1, C14, and C16 (with staminodes explants) prefer protocol IV, while their petal explants prefer protocol II. Also, C9 staminodes explants prefer protocol III while the C9 and C8 petals explants prefer protocol I. This study has identified suitable explants and protocols for somatic embryo production in the tested genotypes. Moreover, this work has laid out a new strategy to overcome recalcitrance to the somatic embryogenesis of T. cacao genotypes.

show abstract

Comparison of systems combining auxins with thidiazuron or kinetin supplemented with polyvinylpirrolidone during embryogenic callus induction in three <i>Theobroma cacao</i> L. genotypes

Cited by 5 publications

References 17 publications

Induction of somatic embryos of recalcitrant genotypes of <i>Theobroma cacao L.</i>

Induction of somatic embryos of recalcitrant genotypes of <i>Theobroma cacao L.</i>

Use of mineral salts to remove recalcitrance to somatic embryogenesis of improved genotypes of cacao (Theobrama cacao L.)

Preferential expression of somatic embryogenesis in five elite genotypes of Theobroma cacao (L.) associated with explant type and protocols used

Contact Info

Product

Resources

About