Comparison of techniques for the extraction of the anti-cancer drug camptothecin from Nothapodytes foetida

Fulzele, Devanand P.; Satdive, Ramesh K.

doi:10.1016/j.chroma.2004.11.020

Cited by 121 publications

(68 citation statements)

References 26 publications

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“…For this reason different procedures have been used for acquisition of biologically active compounds from crude plant extracts. Recent studies mentioned the ultrasonic and microwave methods as efficient in secondary metabolites extraction [5][6][7]. Solvent type and method of extraction are fundamental factors to consider for optimizing yield extraction [8].…”

Section: Introductionmentioning

confidence: 99%

Flavonoid extraction from Alpinia zerumbet (Pers.) Burtt et Smith leaves using different techniques and solvents

2009

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Abstract:The current study aims to verify the best method for a rapid and efficient extraction of flavonoids from Alpinia zerumbet. Dried leaves were extracted using distillated water and ethanol 70% by extraction methods of shaking maceration, ultrasonic, microwave and stirring. By the application of TLC and reversedphase HPLC techniques the rutin and kaempferol-3-O-glucuronide were detected. Ethanol 70% was more efficient for flavonoids extraction than water. No significant yielding variation was verified for ultrasonic, microwave and stirring methods using ethanol 70% (11 to 14%). The relative concentration of rutin and kaempferol-3-O-glucuronide, respectively, was higher by ultrasonic (1.5 and 5.62 mg g -1 dried leaves, respectively) and by microwave (1.0 and 6.64 mg g -1 dried leaves) methods using ethanol. Rapid and simplified extraction proceeding optimize phytochemical work and acquisition of secondary metabolites.Keywords: high performance liquid chromatography; maceration; microwave; ultrasonic; Zingiberaceae.Rio de Janeiro. Voucher specimen was identified and deposited in the Herbarium of Rio de Janeiro Botanical Garden, accession number RB 433485.All chemicals used in analysis as methanol and phosphoric acid were of HPLC grades and were purchased from Merck. MilliQ water was utilized to HPLC mobile phase and sample preparation. Kaempferol-3-O-glucuronide was isolated from Alpinia zerumbet and identified by Nuclear Magnetic Resonance (NMR) [3]. Rutin was purchased from Merck. Preparation of extractsLeaves of A. zerumbet were collected from adult plants, in the morning, then plant material was dried for 3 days in stove (60 o C) and macerated in 70% ethanol or distillated water, in the same proportion of 1 g dried leaves/20 mL solvent (10% w/v).It was evaluated four extraction methods from dried leaves: maceration in shaker at 100 rpm, ultrasonic bath (40 kHz, Thornton Unique, model 1400 USC), microwave (PANASONIC -Auto Sensor Diet, full power) and stirring (Table 1). In the microwave extraction, the suspensions were irradiated under microwaves in pre-setting procedures (3 s power on, 60 s off) for three times to the desired temperature about 60 and 70 o C. The temperature was measured after turn off the microwave using a thermometer into extracts in Becker. For the ultrasonic extraction, the 30 mL flask containing 1 g of dried leaves plus 20 mL of one of extracting solvents was partially immersed into the ultrasonic bath and temperature was controlled.Preliminary results obtained by different extraction time 15 and 30 min for ultrasonic and 1 and 2 days for maceration techniques did not indicate significant differences in relation to extraction time presented in this study.Crude extracts were filtered in vacuum using Whattman ® filter (110 mm ø, 1). Aqueous extracts were frozen, then liophylizated and the hydroalcoholic extracts were evaporated to dry under reduced pressure at 60 o C. The dried weight was measured. The yielding was defined as follows: (crude extract weight/plant material weight) x 100....

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Section: Introductionmentioning

confidence: 99%

Flavonoid extraction from Alpinia zerumbet (Pers.) Burtt et Smith leaves using different techniques and solvents

2009

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“…Microwave-assisted extraction (MAE) is another way of accelerating the extraction of compounds from plant materials. MAE showed high efficiency in extracting essential oils [28][29][30], camptothecin alkaloids [31], and limonoids in neem trees [32]. MAE was also effective in extracting the phytochemicals in peanut [33].…”

Section: Preparation Of Botanical Samples For Initial Screening Testsmentioning

confidence: 99%

Preparation of Botanical Samples for Biomedical Research

Liu

2008

EMIDDT

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Plants are chemical storehouses, a fact which has driven countless multidisciplinary quests for bioactive compounds. As the very first step of botanical research, the whole desire is to find "hit" plants with specific bioactivities. It is logical to use some strategies that can maximize the chances of finding these "hits" with limited time and resources. In addition to selecting the right plants for screening, how the plant extracts are prepared can also influence the bioactivity screening outcomes. An extract from the same plant material can be quite different in chemical composition having different preparations. Because of the complex mixture nature of plant extracts, it is possible artifact activities may be observed. Thus confirmatory activity tests are often necessary to warrant the next laborious isolation step. A bioassay directed isolation approach may be the most efficient in identifying the bioactive compounds because of the narrowed focus at each isolation step, but a phytochemistry isolation approach is appropriate to characterize a purified bioactive extract. In fact, these two approaches can be taken intermittently whenever efficiency can be improved. Finally, use of the identified active compounds is now broader. In addition to determining a lead compound to continue a drug development path, there is an increasing interest in support for the use of botanical extracts as botanical drugs. Instead of dropping the extract after extracting the lead compound, the natural analogues representing the purified extract now have a chance to become leading compounds in the pursuit of novel therapies for metabolic syndrome and other diseases.

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“…The recalcitrant seeds produced by the plants may be considered an alternative source of CPT when they lose viability as many could be found on forest floor few months after seed set. Although in the earlier studies, CPT yield in seed mass of N. nimmoniana found in India was quantified [16][17][18][19][20][21] and new camptothecinoids isolated in Taiwan. 22 The various parts of the seeds and variability in yield of the alkaloid across seed sources to (or "intending to") assessing workable economic use for CPT extraction were not analyzed.…”

Section: Introductionmentioning

confidence: 99%

Should Seed Be an Alternative Source of Camptothecin From Nothapodytes nimmoniana and Chonemorpha fragrance?

Isah¹

2016

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The Southeast Asian forest is blessed with plant sources of anticancer molecules including camptothecin (CPT) but, the irrational harvest is among serious threat to its existence for use by the future generation. Among plant sources of CPT found in the region are Nothapodytes nimmoniana and Chonemorpha fragrance that are under heavy exploitation pressure for the alkaloid and other medicinal purposes. Under the natural conditions of forests, seeds produced by the plants show germination difficulties, and as a result lose viability few months after set. The unviable seeds can be used as an alternative source of CPT and other phytochemicals isolated from the species. In this study, evaluation of CPT yield of seeds and their parts was performed by high-performance thin layer chromatographic analysis to assess the feasible economic use of the seeds as an alternative source of the alkaloid. The results showed a manifold higher yield of CPT in seeds and their parts in N. nimmoniana over C. fragrance, suggesting seeds of the former that face more exploitation of its forest than the latter as a better alternative source of the alkaloid. The results also suggest that collection of the seed should be made before the onset of rain season. In this study, the economic implications of using seeds to isolate CPT are also discussed.

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Comparison of techniques for the extraction of the anti-cancer drug camptothecin from Nothapodytes foetida

Cited by 121 publications

References 26 publications

Flavonoid extraction from Alpinia zerumbet (Pers.) Burtt et Smith leaves using different techniques and solvents

Flavonoid extraction from Alpinia zerumbet (Pers.) Burtt et Smith leaves using different techniques and solvents

Preparation of Botanical Samples for Biomedical Research

Should Seed Be an Alternative Source of Camptothecin From Nothapodytes nimmoniana and Chonemorpha fragrance?

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