Comparison of the adipogenesis in intramuscular and subcutaneous adipocytes from Bamei and Landrace pigs

Hua, ZhangGuo; Xiong, LuJian; ChenYan,; Qing, ZhaoYong; Hui, GuoPeng; Tian, YangJu; Xin, ZangRong

doi:10.1139/bcb-2014-0019

Cited by 37 publications

(42 citation statements)

References 53 publications

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“…Primary cultures of preadipocytes were isolated as previously described (Zhang et al, 2014). Cells were seeded in 25-cm culture flask and maintained in basal medium, DMEM/F12 medium (GIBCO/BRL, USA) supplemented 10% fetal bovine serum (FBS, ScienCell, USA), at 37°C in humidified atmosphere with 5% CO 2 .…”

Section: Methodsmentioning

confidence: 99%

Glucose and Insulin Stimulate Lipogenesis in Porcine Adipocytes: Dissimilar and Identical Regulation Pathway for Key Transcription Factors

Hua

Xiong

Chen

et al. 2016

Molecules and Cells

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Lipogenesis is under the concerted action of ChREBP, SREBP-1c and other transcription factors in response to glucose and insulin. The isolated porcine preadipocytes were differentiated into mature adipocytes to investigate the roles and interrelation of these transcription factors in the context of glucose- and insulin-induced lipogenesis in pigs. In ChREBP-silenced adipocytes, glucose-induced lipogenesis decreased by ~70%, however insulin-induced lipogenesis was unaffected. Moreover, insulin had no effect on ChREBP expression of unperturbed adipocytes irrespective of glucose concentration, suggesting ChREBP mediate glucose-induced lipogenesis. Insulin stimulated SREBP-1c expression and when SREBP-1c activation was blocked, and the insulin-induced lipogenesis decreased by ~55%, suggesting SREBP-1c is a key transcription factor mediating insulin-induced lipogenesis. LXRα activation promoted lipogenesis and lipogenic genes expression. In ChREBP-silenced or SREBP-1c activation blocked adipocytes, LXRα activation facilitated lipogenesis and SREBP-1c expression, but had no effect on ChREBP expression. Therefore, LXRα might mediate lipogenesis via SREBP-1c rather than ChREBP. When ChREBP expression was silenced and SREBP-1c activation blocked simultaneously, glucose and insulin were still able to stimulated lipogenesis and lipogenic genes expression, and LXRα activation enhanced these effects, suggesting LXRα mediated directly glucose- and insulin-induced lipogenesis. In summary, glucose and insulin stimulated lipogenesis through both dissimilar and identical regulation pathway in porcine adipocytes.

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Section: Methodsmentioning

confidence: 99%

Glucose and Insulin Stimulate Lipogenesis in Porcine Adipocytes: Dissimilar and Identical Regulation Pathway for Key Transcription Factors

Hua

Xiong

Chen

et al. 2016

Molecules and Cells

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“…Fat deposition is a complex process related to lipogenesis, adipocyte proliferation and differentiation. Previous research showed that the proliferation, differentiation and lipogenesis capability of preadipocytes was stronger in Bamei (fat-type) pig than in Landrace (lean-type) pig [13]. The intramuscular fat content in Erhualian pig (fattype) was remarkably different at different growth stages, whereas that in Large White pig changed little during growth [15].…”

Section: Pigsmentioning

confidence: 99%

“…They include stearoyl-CoA desaturase (SCD) [7][8][9], fatty acid synthase (FASN) [10,11], sterol regulatory element binding transcription factor 1 (SREBF1) [10], peroxisome proliferator-activated receptor γ (PPARγ) [12]. Some molecular events in the development of intramuscular fat have been recently revealed using longissimus dorsi muscle or adipocytes in pig [11,[13][14][15]. These studies indicate that adipocyte proliferation and differentiation is higher in subcutaneous fat than in intramuscular fat as well as in fat-type pig than in lean-type pig.…”

Section: Introductionmentioning

confidence: 99%

“…LncRNAs, nonprotein coding transcripts longer than 200 nucleotides, have important regulatory functions in adipogenesis and lipid metabolism. With the development of high throughput sequencing technology, RNA-seq has been widely applied in the identification and functional analysis of lncRNAs and mRNAs of adipose tissues in pig [7,13,16,17], chicken [18], sheep [19,20] and cattle [21]. Ramayo et al [22] identified 270 and 186 lncRNAs in the liver tissues of female pigs with high and low intramuscular fatty acid contents, respectively.…”

Section: Introductionmentioning

confidence: 99%

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Genome-Wide Analysis of mRNAs and lncRNAs of Intramuscular Fat Related to Lipid Metabolism in Two Pig Breeds

Huang

Zhang

et al. 2018

Cell Physiol Biochem

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Background/Aims: Long non-coding RNAs (lncRNAs) can regulate adipogenesis and lipid accumulation. Intramuscular fat deposition appears to vary in different pig breeds, and the regulation mechanism has not yet been fully elucidated at molecular level. Moreover, little is known about the function and profile of lncRNAs in intramuscular fat deposition and metabolism in pig. The aim of this study was thus to explore the regulatory functions of lncRNAs in intramuscular fat deposition. Methods: In this study, Laiwu (LW) pig and Large White (LY) pig with significant difference in fat deposition were selected for use. RNA-seq technology and bioinformatics methods were used to comparatively analyze the gene expression profiles of intramuscular fat between LW and LY pigs to identify key mRNAs and lncRNAs associated with lipid metabolism and adipogenesis. Real-time fluorescence-based quantitative PCR was applied to verify the expression level of the differentially expressed mRNAs and lncRNAs. Results: A total of 513 mRNAs and 55 lncRNAs were differentially expressed between two pig breeds. By co-expression network construction as well as cis- and trans-regulated target gene analysis, 31 key lncRNAs were identified. Gene Ontology and KEGG pathway analyses revealed that differentially expressed genes and lncRNAs were mainly involved in the biological processes and pathways related to adipogenesis and lipid metabolism. Conclusion: XLOC_046142, XLOC_004398 and XLOC_015408 may target MAPKAPK2, NR1D2 and AKR1C4, respectively, and play critical regulatory roles in intramuscular adipogenesis and lipid accumulation in pig. XLOC_064871 and XLOC_011001 may play a role in lipid metabolism-related disease via regulating TRIB3 and BRCA1. This study provides a valuable resource for lncRNA study and improves our understanding of the biological roles of lipid metabolism- related genes and molecular mechanism of intramuscular fat metabolism and deposition.

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“…Primers for genes important for insulin responsiveness of adipose tissue were also examined and included sterol regulatory element-binding protein 1 (SREBP), carbohydrate regulatory element-binding protein (ChREBP) and insulin receptor substrate 1 (IRS1). Primers for SREBP were obtained from Gondret et al (2011), whereas primers for ChREBP were acquired from Zhang et al (2014). IRS1 primers were derived from Jiao et al (2011).…”

Section: Agp and Lipogenesis In Neonatal Pigsmentioning

confidence: 99%

α 1-acid glycoprotein inhibits lipogenesis in neonatal swine adipose tissue

Ramsay

Blomberg

Caperna

2016

Animal

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Serum α1-acid glycoprotein (AGP) is elevated during late gestation and at birth in the pig and rapidly declines postnatally. In contrast, the pig is born with minimal lipid stores in the adipose tissue, but rapidly accumulates lipid during the first week. The present study examined if AGP can affect adipose tissue metabolism in the neonatal pig. Isolated cell cultures or tissue explants were prepared from dorsal subcutaneous adipose tissue of preweaning piglets. Porcine AGP was used at concentrations of 0, 100, 1000 and 5000 ng/ml medium in 24 h incubations. AGP reduced the messenger RNA (mRNA) abundance of the lipogenic enzymes, malic enzyme (ME), fatty acid synthase and acetyl coA carboxylase by at least 40% ( P < 0.001). The activity of ME and citrate lyase were also reduced by AGP ( P < 0.05). Glucose oxidation was reduced by treatment with 5000 ng AGP/ml medium ( P < 0.05). The 14 C-glucose incorporation into fatty acids was reduced by~25% by AGP treatment for 24 h with 1000 ng AGP/ml medium ( P < 0.05). The decrease in glucose metabolism by AGP appears to function through an inhibition in insulin-mediated glucose oxidation and incorporation into fatty acids. This was supported by the analysis of the mRNA abundance for sterol regulatory element-binding protein (SREBP), carbohydrate regulatory element-binding protein (ChREBP) and insulin receptor substrate 1 (IRS1), which all demonstrated reductions of at least 23% in response to AGP treatment ( P < 0.05). These data demonstrate an overall suppression of lipogenesis due to AGP inhibition of lipogenic gene expression in vitro, which the metabolic data and SREBP, ChREBP and IRS1 gene expression analysis suggest is through an inhibition in insulin-mediated events. Second, these data suggest that AGP may contribute to limiting lipogenesis within adipose tissue during the perinatal period, as AGP levels are highest for any serum protein at birth.

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Comparison of the adipogenesis in intramuscular and subcutaneous adipocytes from Bamei and Landrace pigs

Cited by 37 publications

References 53 publications

Glucose and Insulin Stimulate Lipogenesis in Porcine Adipocytes: Dissimilar and Identical Regulation Pathway for Key Transcription Factors

Glucose and Insulin Stimulate Lipogenesis in Porcine Adipocytes: Dissimilar and Identical Regulation Pathway for Key Transcription Factors

Genome-Wide Analysis of mRNAs and lncRNAs of Intramuscular Fat Related to Lipid Metabolism in Two Pig Breeds

α 1-acid glycoprotein inhibits lipogenesis in neonatal swine adipose tissue

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