Comparison of the adolescent and adult mouse prefrontal cortex proteome

Agoglia, Abigail E.; Holstein, Sarah E.; Small, Amanda; Spanos, Marina; Burrus, Brainard

doi:10.1371/journal.pone.0178391

Cited by 14 publications

(26 citation statements)

References 75 publications

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“…Interestingly, whereas Agoglia et al (2017) reported enhanced protein alterations in networks that regulate neuronal signaling, anxiety-related behavior and neurological disease in the adolescent PFC when compared to adult mice, our gene expression results found similar associations but only in adults. This different outcome may be due to differences in the age-stage comparison between studies.…”

Section: Discussionmentioning

confidence: 42%

“…The majority of these analyzes conducted in rodents focus on the study of the molecular changes occurring in post-natal and pubertyadolescence stages. However, studies on the specific changes of genetic expression during adulthood are still scarce (Agoglia et al, 2017;Lander et al, 201712). In the present study, we used RNA-seq to identify genes and gene networks that show differential expression in the PFC of adult bulls when compared to young bovines of the aggressive Lidia breed.…”

Section: Discussionmentioning

confidence: 99%

“…Agoglia et al (2017) used an unbiased proteomics approach to analyze the differential expression of proteins in adolescent compared to adult PFC in mouse. There is a concordance between the proportion of up and down-regulated DEGs in the adult groups reported by both us andAgoglia et al (2017), as well as results from the GO analyses showing down-regulation of organismal growth and development processes in adults. This means that both technologies, proteomics and RNA-seq are, as awaited, correlated, detecting similar sets of DE molecules(Kogenaru et al, 2012).…”

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confidence: 99%

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Age effects aggressive behavior: RNA-seq analysis in cattle with implications for studying neoteny under domestication

Eusebi

Sevane

O’Rourke

et al. 2020

Preprint

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Aggressiveness is one of our most basic behaviors, characterized by targeted intentional actions oriented to cause harm or damage. The reactive type of aggression is regulated mainly in the brain prefrontal cortex; however, the molecular changes underlying aggressiveness in adults have not been fully characterized. Here we used a RNA-seq approach to investigate differential gene expression in the prefrontal cortex of bovines from the aggressive Lidia breed at different age stages: young three-year old and adult four-year-old bulls. The number of up and down-regulated genes in the adult group were 50 and 193, respectively. These genes were associated with diverse functions, including neural plasticity and behavior. A cross-species comparative analysis retrieved 29 genes in common with previous studies on aggressive behaviors. Particularly, we detected changes in the regulation of networks such as the synaptogenesis, involved in the synapses maintenance and refinement, and the glutamate receptor pathway, which acts as excitatory driver in aggression responses. Furthermore, the Wnt//β- catenin signaling pathway was present in both up and down-regulated genes in the adult group, and has been implicated in adult altered behaviors and cognitive disorders. At the regulatory level, we identified four upstream regulators, all involved in behavioral processes and neuropsychiatric disorders including aggressive demonstrations. Our results on an animal model in which the aging process enhance the intensity of aggressive responses provide, for the first time, insights into candidate genes and networks involved in the molecular mechanisms leading to the maturation of the brain, with a focus on cattle agonistic components.

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Section: Discussionmentioning

confidence: 42%

Section: Discussionmentioning

confidence: 99%

mentioning

confidence: 99%

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Age effects aggressive behavior: RNA-seq analysis in cattle with implications for studying neoteny under domestication

Eusebi

Sevane

O’Rourke

et al. 2020

Preprint

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“…Given the importance of the mPFC in higher order cognitive functions and memory processes as well as in brain diseases, particularly in neurodevelopmental diseases, it is surprising that relatively little has been systematically investigated about cellular and molecular biological mechanisms underlying its development and/or maturation of its functional responses. A few studies have carried out proteomic analyses on whole mouse mPFC protein fraction (Laeremans et al 2013;Gonzalez-Lozano et al 2016;Agoglia et al 2017) or on both mouse and rat mPFC synaptosomal fraction (Counotte et al 2010;Moczulska et al 2014) over developmental ages. However, much remains to be investigated.…”

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confidence: 99%

Developmental changes in plasticity, synaptic, glia, and connectivity protein levels in rat medial prefrontal cortex

et al. 2018

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The medial prefrontal cortex (mPFC) plays a critical role in complex brain functions including decision-making, integration of emotional, and cognitive aspects in memory processing and memory consolidation. Because relatively little is known about the molecular mechanisms underlying its development, we quantified rat mPFC basal expression levels of sets of plasticity, synaptic, glia, and connectivity proteins at different developmental ages. Specifically, we compared the mPFC of rats at postnatal day 17 (PN17), when they are still unable to express long-term contextual and spatial memories, to rat mPFC at PN24, when they have acquired the ability of long-term memory expression and finally to the mPFC of adult rats. We found that, with increased age, there are remarkable and significant decreases in markers of cell activation and significant increases in proteins that mark synaptogenesis and synapse maturation. Furthermore, we found significant changes in structural markers over the ages, suggesting that structural connectivity of the mPFC increases over time. Finally, the substantial biological difference in mPFC at different ages suggest caution in extrapolating conclusions from brain plasticity studies conducted at different developmental stages.

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“…Therefore, it is possible that training in the attentional task was a chronic mild stress for the α9-nAChR KO mice. In this context, chronic stress alters mice mPFC, a brain area involved on inhibitory control (Gilabert-Juan et al, 2013 ; Agoglia et al, 2017 ). Together, these findings suggest that in our experiments training stress might have deteriorated the mPFC of the α9-nAChR KO.…”

Section: Discussionmentioning

confidence: 99%

Difference in Perseverative Errors during a Visual Attention Task with Auditory Distractors in Alpha-9 Nicotinic Receptor Subunit Wild Type and Knock-Out Mice

Jorratt

Délano

Delgado

et al. 2017

Front. Cell. Neurosci.

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The auditory efferent system is a neural network that originates in the auditory cortex and projects to the cochlear receptor through olivocochlear (OC) neurons. Medial OC neurons make cholinergic synapses with outer hair cells (OHCs) through nicotinic receptors constituted by α9 and α10 subunits. One of the physiological functions of the α9 nicotinic receptor subunit (α9-nAChR) is the suppression of auditory distractors during selective attention to visual stimuli. In a recent study we demonstrated that the behavioral performance of alpha-9 nicotinic receptor knock-out (KO) mice is altered during selective attention to visual stimuli with auditory distractors since they made less correct responses and more omissions than wild type (WT) mice. As the inhibition of the behavioral responses to irrelevant stimuli is an important mechanism of the selective attention processes, behavioral errors are relevant measures that can reflect altered inhibitory control. Errors produced during a cued attention task can be classified as premature, target and perseverative errors. Perseverative responses can be considered as an inability to inhibit the repetition of an action already planned, while premature responses can be considered as an index of the ability to wait or retain an action. Here, we studied premature, target and perseverative errors during a visual attention task with auditory distractors in WT and KO mice. We found that α9-KO mice make fewer perseverative errors with longer latencies than WT mice in the presence of auditory distractors. In addition, although we found no significant difference in the number of target error between genotypes, KO mice made more short-latency target errors than WT mice during the presentation of auditory distractors. The fewer perseverative error made by α9-KO mice could be explained by a reduced motivation for reward and an increased impulsivity during decision making with auditory distraction in KO mice.

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Comparison of the adolescent and adult mouse prefrontal cortex proteome

Cited by 14 publications

References 75 publications

Age effects aggressive behavior: RNA-seq analysis in cattle with implications for studying neoteny under domestication

Age effects aggressive behavior: RNA-seq analysis in cattle with implications for studying neoteny under domestication

Developmental changes in plasticity, synaptic, glia, and connectivity protein levels in rat medial prefrontal cortex

Difference in Perseverative Errors during a Visual Attention Task with Auditory Distractors in Alpha-9 Nicotinic Receptor Subunit Wild Type and Knock-Out Mice

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