Comparison of the Amounts of hCG and PMSG to Induce Ovulation in 50% of the Animals, Mice, Syrian Hamsters and Rats

Yamaguchi, Takao; Katsuyama, Makoto; Suzuki, Wataru; Saitô, Tôru; Takahashi, Kazuaki W.

doi:10.1538/expanim1978.41.2_153

Experimental Animals

1992

DOI: 10.1538/expanim1978.41.2_153

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Comparison of the Amounts of hCG and PMSG to Induce Ovulation in 50% of the Animals, Mice, Syrian Hamsters and Rats

Takao Yamaguchi¹,

Makoto Katsuyama²,

Wataru Suzuki

et al.

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1997

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Superovulation Induction in the House Musk Shrew(Suncus murinus).

Matsuzaki

Yokoyama

et al. 1997

Exp. Anim.

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The number of ovulated oocytes in the adult house musk shrews was examined by natural mating. The positive rate of ovulation was 83.3%, and the mean number of ovulated oocytes was 4.4 +/- 1.9 (Mean +/- SD). The induction of superovulation was examined by administering either pregnant mare's serum gonadotropin (PMSG) or human chorionic gonadotropin (hCG), exogenous gonadotropin, to house musk shrews. As a result, the number of ovulated oocytes were within the number of oocytes ovulated spontaneously. The method of superovulation induction by administering both PMSG and hCG to house musk shrews was investigated. In the group intraperitoneally with 7.5 i.u. of PMSG followed by 7.5 i.u. of hCG 48 hr later, the positive rate of ovulation was as high as 94.6% and the mean number of ovulated oocytes was 11.7 +/- 13.4. Moreover, in 15 out of these 35 animals positive for ovulation, 10 oocytes or more were observed and the mean number of ovulated oocytes was 21.0 +/- 16.5, showing the superovulatory response. In the group of 5.0 i.u. PMSG and 5.0 i.u. hCG injected at an interval of 72 hr, every animal was induced to ovulate and the mean number of ovulated oocytes was 41.3 +/- 19.9. Thus, the injection of PMSG and hCG at a longer interval increased the number of superovulated oocytes. Furthermore, 97.5% of eggs recovered from superovulating animals contained cumulus cells.

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Superovulation Induction in the House Musk Shrew(Suncus murinus).

Matsuzaki

Yokoyama

et al. 1997

Exp. Anim.

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Comparison of the Amounts of hCG and PMSG to Induce Ovulation in 50% of the Animals, Mice, Syrian Hamsters and Rats

Cited by 1 publication

References 0 publications

Superovulation Induction in the House Musk Shrew(Suncus murinus).

Superovulation Induction in the House Musk Shrew(Suncus murinus).

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