Comparison of the Amounts of Aflatoxin Extracted From Raw Peanuts Using AOAC Methods I and II1

Abstract

Four lots of raw shelled peanuts, naturally contaminated with aflatoxin, were each ground into a paste. Sixty-four 50-g samples were removed from three of the lots and forty 50-g samples were removed from the fourth lot. For each lot, aflatoxin was extracted from half of the samples by the AOAC Method I (CB) and from the remaining half by the AOAC Method II (BF). The 4 lots averaged 57.8, 127.6, 238.5, and 447.1 parts per billion (ppb) total aflatoxin when measured by the CB method. On the average across the four lots, the BF method extracted 26, 25, 22, and 18% less aflatoxin B1, B2, G1, and G2, respectively, than the CB method.