Comparison of the aneugenic effect of vinorelbine and vincristine in cultured human lymphocytes

González-Cid, Marcela; Cuello, Maria Teresa; Larripa, Irene

doi:10.1093/mutage/14.1.63

Cited by 30 publications

(11 citation statements)

References 27 publications

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“…Sterile water was used as the negative control. The mutagenic compounds were used at the following final concentrations: EMS (Sigma), 120 mg/ml [Fimognari et al, 2001]; VIN (Sigma), 0.05 mg/ml [González-Cid et al, 1999]; H 2 O 2 (Sigma), 3.4 mg/ml [Fenech et al, 1999a]; MMC (Sigma), 0.1 mg/ml [Caria et al, 1997].…”

Section: Cell Treatmentsmentioning

confidence: 99%

Effect of sulforaphane on micronucleus induction in cultured human lymphocytes by four different mutagens

Fimognari

Berti

Cantelli‐Forti

et al. 2005

Environ. Mol. Mutagen.

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Isothiocyanates (ITCs) are commonly found in cruciferous vegetables. A variety of biological activities have been ascribed to ITCs, such as inhibition of cytochrome P450 enzymes and induction of phase II enzymes in animal models. ITCs are also able to block cell-cycle progression and induce apoptosis in human cancer cells in vitro. In this study, we evaluated the ability of the ITC sulforaphane to protect cultured human lymphocytes from micronucleus (MN) induction by four different mutagens: ethyl methanesulfonate (EMS), vincristrine (VIN), H(2)O(2) and mitomycin C (MMC). To understand the mechanisms of action of sulforaphane, the cultures were treated with the compound before, during and after treatment with the mutagens; in addition, the cultures were evaluated for the induction of apoptosis. Up to 10 microM, sulforaphane was non-genotoxic by itself, while 30 microM sulforaphane reduced the replicative index of the cells by more than 60%. Moreover, 1-10 microM sulforaphane reduced the MN frequency induced by EMS, VIN, H(2)O(2) and MMC in at least one of the treatment protocols; it had no effect on H(2)O(2)-MN induction in the post-treatment protocol, and it increased MN induction by MMC in the pre-treatment protocol. Apoptosis was produced in the cultures treated with sulforaphane alone. The fraction of apoptotic cells was increased after co- or post-treatment with sulforaphane and EMS and MMC, suggesting that sulforaphane-mediated apoptosis may remove highly damaged cells induced by these agents. Other mechanisms are involved in the anti-genotoxic activity of sulforaphane against VIN and H(2)O(2). Taken together, our findings indicate that under certain conditions sulforaphane possesses anti-genotoxic activity in vitro and that further studies are warranted to characterize this property in vivo.

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Section: Cell Treatmentsmentioning

confidence: 99%

Effect of sulforaphane on micronucleus induction in cultured human lymphocytes by four different mutagens

Fimognari

Berti

Cantelli‐Forti

et al. 2005

Environ. Mol. Mutagen.

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“…The induction of MnCþ could be attributed to the ability of Al to modify the structure of tubulin (Sivaguru et al, 2003). as it is well known that agents that damage mitotic spindles induce aneuploidy (Gonzalez-Cid et al, 1999).…”

Section: Introductionmentioning

confidence: 99%

Aluminum-induced micronuclei and apoptosis in human peripheral-blood lymphocytes treated during different phases of the cell cycle

et al. 2005

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Although aluminum (Al) is responsible for the etiology of some human diseases, not much is known about the mechanisms of its genotoxic activity. The available data suggest that Al can induce DNA damage by modifying the structure of chromatin through the induction of reactive oxygen species or by damaging lysosomal membranes and liberating DNase. We treated human peripheral-blood lymphocytes with AlCl3 in the G0/G1 phase, in the S/G2 phase, and during the whole cell cycle. The aim of the study was to check if the sensitivity of lymphocytes to Al varied through the cell cycle. A high sensitivity in the S phase would point toward chromatin modification as the major source of DNA damage. Micronuclei (Mn) and apoptosis were assessed as the end points. Cells were treated with 1, 2, 5, 10, and 25 microg/mL AlCl3. Mn induced by 5 microg/mL of AlCl3 were analyzed by FISH for centromeric signal content. After all treatment schemes the frequency of Mn increased initially, but decreased at high AlCl3 concentrations. This drop of Mn frequency could be explained by a strong increase in the frequency of apoptosis. AlCl3 induced both Mn with and without centromeres. The G0/G1 phase of the cell cycle was found to be more sensitive than were the S and G2 phases. This points toward oxidative stress or liberation of DNase as the major source of DNA damage induced by Al.

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“…As different investigators relate MN appearance with polyploidy and aneuploidy in mammalian cells caused by different genotoxic compounds or treatment (Ciranni et al, 1995;Uryvaeva and Delone, 1995;Elhajouji et al, 1998;Miller et al, 1998;Gonzalez-Cid et al, 1999), the aim of our study was to establish MN appearance in triploid rainbow trout. To the best of the author's knowledge this is the first study of this kind conducted on triploid fish.…”

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confidence: 99%

Micronucleus occurrence in diploid and triploid rainbow trout (Oncorhynchus mykiss Walbaum)

Strunjak‐Perović¹,

Čož‐Rakovac²,

Popović³

2003

Vet. Med. - Czech

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ABSTRACT:The aim of the study was to observe the influence of different ploidy levels in fish on micronucleus occurrence. Twenty minutes a�er fertilization, one group of rainbow trout eggs was exposed to water temperatures of 26°C in duration of 20 minutes to induce triploidy. Second group was kept in water temperature of 10°C, which is optimal for development of rainbow trout. The frequency of micronucleated erythrocytes was determined in the peripheral circulation of rainbow trout 67 days (following absorption of the yolk -swim-up stage) and 128 days (fry stage) post fertilization. There was a significant difference (P < 0.001) between frequency of micronucleated erythrocytes of diploid (1.10 ± 0.96‰) and triploid (2.41 ± 1.28‰) fish at swim-up stage. Increased mean values of micronucleus in diploid (1.80 ± 1.57‰) and triploid (5.92 ± 3.80‰) fry were also recorded.

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Comparison of the aneugenic effect of vinorelbine and vincristine in cultured human lymphocytes

Cited by 30 publications

References 27 publications

Effect of sulforaphane on micronucleus induction in cultured human lymphocytes by four different mutagens

Effect of sulforaphane on micronucleus induction in cultured human lymphocytes by four different mutagens

Aluminum-induced micronuclei and apoptosis in human peripheral-blood lymphocytes treated during different phases of the cell cycle

Micronucleus occurrence in diploid and triploid rainbow trout (Oncorhynchus mykiss Walbaum)

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